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Preparation of developing and adult Drosophila brains and retinae for live imaging.

Williamson WR, Hiesinger PR - J Vis Exp (2010)

Bottom Line: However, we also present the conditions for fixed tissue immunohistochemistry where applicable.Finally, we show live imaging conditions for these preparations using conventional and resonant 4D confocal live imaging in a perfusion chamber.Together, these protocols provide a basis for live imaging on different time scales ranging from functional intracellular assays on the scale of minutes to developmental or degenerative processes on the scale of many hours.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology and Green Center for Systems Biology, University of Texas Southwestern Medical Center, USA.

ABSTRACT
The Drosophila brain and visual system are widely utilized model systems to study neuronal development, function and degeneration. Here we show three preparations of the brain and visual system that cover the range from the developing eye disc-brain complex in the developing pupae to individual eye and brain dissection from adult flies. All protocols are optimized for the live culture of the preparations. However, we also present the conditions for fixed tissue immunohistochemistry where applicable. Finally, we show live imaging conditions for these preparations using conventional and resonant 4D confocal live imaging in a perfusion chamber. Together, these protocols provide a basis for live imaging on different time scales ranging from functional intracellular assays on the scale of minutes to developmental or degenerative processes on the scale of many hours.

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Related in: MedlinePlus

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Preparation of developing and adult Drosophila brains and retinae for live imaging.

Williamson WR, Hiesinger PR - J Vis Exp (2010)

© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2926941&req=5

Bottom Line: However, we also present the conditions for fixed tissue immunohistochemistry where applicable.Finally, we show live imaging conditions for these preparations using conventional and resonant 4D confocal live imaging in a perfusion chamber.Together, these protocols provide a basis for live imaging on different time scales ranging from functional intracellular assays on the scale of minutes to developmental or degenerative processes on the scale of many hours.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology and Green Center for Systems Biology, University of Texas Southwestern Medical Center, USA.

ABSTRACT
The Drosophila brain and visual system are widely utilized model systems to study neuronal development, function and degeneration. Here we show three preparations of the brain and visual system that cover the range from the developing eye disc-brain complex in the developing pupae to individual eye and brain dissection from adult flies. All protocols are optimized for the live culture of the preparations. However, we also present the conditions for fixed tissue immunohistochemistry where applicable. Finally, we show live imaging conditions for these preparations using conventional and resonant 4D confocal live imaging in a perfusion chamber. Together, these protocols provide a basis for live imaging on different time scales ranging from functional intracellular assays on the scale of minutes to developmental or degenerative processes on the scale of many hours.

Show MeSH
Related in: MedlinePlus