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Oral microencapsulated live Saccharomyces cerevisiae cells for use in renal failure uremia: preparation and in vivo analysis.

Coussa R, Martoni C, Bhathena J, Urbanska AM, Prakash S - J. Biomed. Biotechnol. (2010)

Bottom Line: Orally administrating these microcapsules to uremic rats was found to decrease urea concentrations from 7.29 +/- 0.89 mmol/L to 6.12 +/- 0.90 mmol/L over a treatment period of eight weeks.While creatinine concentrations for both uremic-control and uremic-treatment groups did not differ among each other (P > .05), they were, however, significantly higher than those of the normal control group (P < .05).There were no significant differences in both calcium and phosphate concentrations among all three groups (P > .05).

View Article: PubMed Central - PubMed

Affiliation: Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, Faculty of Medicine, McGill University, Montréal, QC, Canada H3A 2B4.

ABSTRACT
Orally administrable alginate-poly-L-lysine-alginate (APA) microcapsules containing live yeast cells was investigated for use in renal failure. At all times, yeast cells remain inside the microcapsules, which are then excreted in the stool. During their gastrointestinal passage, small molecules, like urea, diffuse into the yeast microcapsules where they are hydrolyzed. Orally administrating these microcapsules to uremic rats was found to decrease urea concentrations from 7.29 +/- 0.89 mmol/L to 6.12 +/- 0.90 mmol/L over a treatment period of eight weeks. After stopping the treatment, the urea concentrations increased back to uremic levels of 7.64 +/- 0.77 mmol/L. The analysis of creatinine concentrations averaged 39.19 +/- 4.33 micromol/L, 50.83 +/- 5.55 micromol/L, and 50.28 +/- 7.10 micromol/L for the normal-control, uremic-control and uremic-treatment groups, respectively. While creatinine concentrations for both uremic-control and uremic-treatment groups did not differ among each other (P > .05), they were, however, significantly higher than those of the normal control group (P < .05). Uric acid concentrations averaged 80.08 +/- 26.49 micromol/L, 99.92 +/- 26.55 micromol/L, and 86.49 +/- 28.42 micromol/L for the normal-control, uremic-control and uremic-treatment groups, respectively. There were no significant differences in both calcium and phosphate concentrations among all three groups (P > .05). The microbial populations of five tested types of bacteria were not substantially altered by the presence of the yeast APA encapsulated yeast (P > .05).

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Rats' body weight before, during and after oral administration of APA microencapsulated yeast cells.
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Related In: Results  -  Collection


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fig1: Rats' body weight before, during and after oral administration of APA microencapsulated yeast cells.

Mentions: One of the important parameters indicating the efficiency of the treatment is the body weight, which was measured twice a week along the trial period. Figure 1 shows the average body weights of each studied group. The average body weight of the normal control group increased from 347.83 ± 11.86 g at t = 1 week to 464.03 ± 11.86 g at t = 11 weeks, respectively. Similarly, body weights for the uremic control and uremic treatment groups increased from 341.64 ± 11.86 g to 461.73 ± 29.01 g, and from 345.50 ± 9.28 g to 465.00 ± 34.56 g, respectively, between week 1 and 11 of the trial. There were significant time and group effects in rats' body weight (P < .05). The urea concentration, which is one of the essential indicators of the success of the proposed treatment, followed a different trend (Figure 2). Specifically, urea levels in the uremic treatment group during the pre-TP and post-TP phases were not statistically different from those for the uremic control group (P > .05). However, urea concentrations for the uremic treatment group in between weeks 2 and 9 were significantly different from those for the other two controls (P < .05). All along the 11 weeks of the trial, urea concentrations averaged 5.37 ± 0.67 mmol/L and 7.39 ± 0.99 mmol/L for the normal and uremic control groups, respectively. The average urea concentration for the uremic treatment group decreased from 7.29 ± 0.89 mmol/L to 6.97 ± 0.48 mmol/L after 1 week of treatment. During the treatment, the urea concentration for this same group averaged 6.01 ± 0.47 mmol/L before increasing again to 7.64 ± 0.77 mmol/L once the gavage had stopped.


Oral microencapsulated live Saccharomyces cerevisiae cells for use in renal failure uremia: preparation and in vivo analysis.

Coussa R, Martoni C, Bhathena J, Urbanska AM, Prakash S - J. Biomed. Biotechnol. (2010)

Rats' body weight before, during and after oral administration of APA microencapsulated yeast cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2926829&req=5

fig1: Rats' body weight before, during and after oral administration of APA microencapsulated yeast cells.
Mentions: One of the important parameters indicating the efficiency of the treatment is the body weight, which was measured twice a week along the trial period. Figure 1 shows the average body weights of each studied group. The average body weight of the normal control group increased from 347.83 ± 11.86 g at t = 1 week to 464.03 ± 11.86 g at t = 11 weeks, respectively. Similarly, body weights for the uremic control and uremic treatment groups increased from 341.64 ± 11.86 g to 461.73 ± 29.01 g, and from 345.50 ± 9.28 g to 465.00 ± 34.56 g, respectively, between week 1 and 11 of the trial. There were significant time and group effects in rats' body weight (P < .05). The urea concentration, which is one of the essential indicators of the success of the proposed treatment, followed a different trend (Figure 2). Specifically, urea levels in the uremic treatment group during the pre-TP and post-TP phases were not statistically different from those for the uremic control group (P > .05). However, urea concentrations for the uremic treatment group in between weeks 2 and 9 were significantly different from those for the other two controls (P < .05). All along the 11 weeks of the trial, urea concentrations averaged 5.37 ± 0.67 mmol/L and 7.39 ± 0.99 mmol/L for the normal and uremic control groups, respectively. The average urea concentration for the uremic treatment group decreased from 7.29 ± 0.89 mmol/L to 6.97 ± 0.48 mmol/L after 1 week of treatment. During the treatment, the urea concentration for this same group averaged 6.01 ± 0.47 mmol/L before increasing again to 7.64 ± 0.77 mmol/L once the gavage had stopped.

Bottom Line: Orally administrating these microcapsules to uremic rats was found to decrease urea concentrations from 7.29 +/- 0.89 mmol/L to 6.12 +/- 0.90 mmol/L over a treatment period of eight weeks.While creatinine concentrations for both uremic-control and uremic-treatment groups did not differ among each other (P > .05), they were, however, significantly higher than those of the normal control group (P < .05).There were no significant differences in both calcium and phosphate concentrations among all three groups (P > .05).

View Article: PubMed Central - PubMed

Affiliation: Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, Faculty of Medicine, McGill University, Montréal, QC, Canada H3A 2B4.

ABSTRACT
Orally administrable alginate-poly-L-lysine-alginate (APA) microcapsules containing live yeast cells was investigated for use in renal failure. At all times, yeast cells remain inside the microcapsules, which are then excreted in the stool. During their gastrointestinal passage, small molecules, like urea, diffuse into the yeast microcapsules where they are hydrolyzed. Orally administrating these microcapsules to uremic rats was found to decrease urea concentrations from 7.29 +/- 0.89 mmol/L to 6.12 +/- 0.90 mmol/L over a treatment period of eight weeks. After stopping the treatment, the urea concentrations increased back to uremic levels of 7.64 +/- 0.77 mmol/L. The analysis of creatinine concentrations averaged 39.19 +/- 4.33 micromol/L, 50.83 +/- 5.55 micromol/L, and 50.28 +/- 7.10 micromol/L for the normal-control, uremic-control and uremic-treatment groups, respectively. While creatinine concentrations for both uremic-control and uremic-treatment groups did not differ among each other (P > .05), they were, however, significantly higher than those of the normal control group (P < .05). Uric acid concentrations averaged 80.08 +/- 26.49 micromol/L, 99.92 +/- 26.55 micromol/L, and 86.49 +/- 28.42 micromol/L for the normal-control, uremic-control and uremic-treatment groups, respectively. There were no significant differences in both calcium and phosphate concentrations among all three groups (P > .05). The microbial populations of five tested types of bacteria were not substantially altered by the presence of the yeast APA encapsulated yeast (P > .05).

Show MeSH
Related in: MedlinePlus