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Use of Tetra-ammonium Tetrakis(4-Sulphonato)Phenyl Porphyrin for Pseudomonas and Bacillus Cell Imaging.

Sujatha V, Sridhar B, Krishnamurthy S, Vinod Kumar KS, Senthil Kumar K, Gautam P - Int J Anal Chem (2010)

Bottom Line: TPPS was effectively used to analyse two different bacteria: Pseudomonas aeruginosa and Bacillus cereus.The variation in brightness with varying concentrations of TPPS was studied.The patterns of variations for these bacteria were found to be the same, but with consistently higher brightness for Bacillus cereus.

View Article: PubMed Central - PubMed

Affiliation: Center for Biotechnology, Anna University, Chennai 600025, India.

ABSTRACT
The use of tetraammonium tetrakis(4-sulphonato)phenyl porphyrin (TPPS), a water-soluble anionic compound, as a stain to analyse bacterial cells using fluorescent microscopy was investigated. TPPS was effectively used to analyse two different bacteria: Pseudomonas aeruginosa and Bacillus cereus. The variation in brightness with varying concentrations of TPPS was studied. The patterns of variations for these bacteria were found to be the same, but with consistently higher brightness for Bacillus cereus.

No MeSH data available.


Variation of Brightness with concentration of TPPS for Bacillus cereus and Pseudomonas aeruginosa cells.
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Related In: Results  -  Collection


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fig3: Variation of Brightness with concentration of TPPS for Bacillus cereus and Pseudomonas aeruginosa cells.

Mentions: Image analysis revealed that the brightness increases with increasing concentrations of TPPS for both Pseudomonas aeruginosa and Bacillus cereus before achieving saturation. The concentration at which the brightness achieves saturation is about the same for both OF the bacteria (0.15–0.25 mg/mL), but the brightness is consistently higher for Bacillus cereus (Figure 3). This indicates that the uptake of TPPS by the Gram-positive bacteria is much higher than that of the Gram-negative bacteria.


Use of Tetra-ammonium Tetrakis(4-Sulphonato)Phenyl Porphyrin for Pseudomonas and Bacillus Cell Imaging.

Sujatha V, Sridhar B, Krishnamurthy S, Vinod Kumar KS, Senthil Kumar K, Gautam P - Int J Anal Chem (2010)

Variation of Brightness with concentration of TPPS for Bacillus cereus and Pseudomonas aeruginosa cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2926579&req=5

fig3: Variation of Brightness with concentration of TPPS for Bacillus cereus and Pseudomonas aeruginosa cells.
Mentions: Image analysis revealed that the brightness increases with increasing concentrations of TPPS for both Pseudomonas aeruginosa and Bacillus cereus before achieving saturation. The concentration at which the brightness achieves saturation is about the same for both OF the bacteria (0.15–0.25 mg/mL), but the brightness is consistently higher for Bacillus cereus (Figure 3). This indicates that the uptake of TPPS by the Gram-positive bacteria is much higher than that of the Gram-negative bacteria.

Bottom Line: TPPS was effectively used to analyse two different bacteria: Pseudomonas aeruginosa and Bacillus cereus.The variation in brightness with varying concentrations of TPPS was studied.The patterns of variations for these bacteria were found to be the same, but with consistently higher brightness for Bacillus cereus.

View Article: PubMed Central - PubMed

Affiliation: Center for Biotechnology, Anna University, Chennai 600025, India.

ABSTRACT
The use of tetraammonium tetrakis(4-sulphonato)phenyl porphyrin (TPPS), a water-soluble anionic compound, as a stain to analyse bacterial cells using fluorescent microscopy was investigated. TPPS was effectively used to analyse two different bacteria: Pseudomonas aeruginosa and Bacillus cereus. The variation in brightness with varying concentrations of TPPS was studied. The patterns of variations for these bacteria were found to be the same, but with consistently higher brightness for Bacillus cereus.

No MeSH data available.