Limits...
Use of Tetra-ammonium Tetrakis(4-Sulphonato)Phenyl Porphyrin for Pseudomonas and Bacillus Cell Imaging.

Sujatha V, Sridhar B, Krishnamurthy S, Vinod Kumar KS, Senthil Kumar K, Gautam P - Int J Anal Chem (2010)

Bottom Line: TPPS was effectively used to analyse two different bacteria: Pseudomonas aeruginosa and Bacillus cereus.The variation in brightness with varying concentrations of TPPS was studied.The patterns of variations for these bacteria were found to be the same, but with consistently higher brightness for Bacillus cereus.

View Article: PubMed Central - PubMed

Affiliation: Center for Biotechnology, Anna University, Chennai 600025, India.

ABSTRACT
The use of tetraammonium tetrakis(4-sulphonato)phenyl porphyrin (TPPS), a water-soluble anionic compound, as a stain to analyse bacterial cells using fluorescent microscopy was investigated. TPPS was effectively used to analyse two different bacteria: Pseudomonas aeruginosa and Bacillus cereus. The variation in brightness with varying concentrations of TPPS was studied. The patterns of variations for these bacteria were found to be the same, but with consistently higher brightness for Bacillus cereus.

No MeSH data available.


Fluorescent microscopy images of Bacillus cereus (a) and Pseudomonas aeruginosa (b).
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2926579&req=5

fig2: Fluorescent microscopy images of Bacillus cereus (a) and Pseudomonas aeruginosa (b).

Mentions: The cells with TPPS fluoresced, when visualised under fluorescent microscope (Figure 2) with excitation at 488 nm wavelength, while control cells to which TPPS was not added did not show any fluorescence.


Use of Tetra-ammonium Tetrakis(4-Sulphonato)Phenyl Porphyrin for Pseudomonas and Bacillus Cell Imaging.

Sujatha V, Sridhar B, Krishnamurthy S, Vinod Kumar KS, Senthil Kumar K, Gautam P - Int J Anal Chem (2010)

Fluorescent microscopy images of Bacillus cereus (a) and Pseudomonas aeruginosa (b).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2926579&req=5

fig2: Fluorescent microscopy images of Bacillus cereus (a) and Pseudomonas aeruginosa (b).
Mentions: The cells with TPPS fluoresced, when visualised under fluorescent microscope (Figure 2) with excitation at 488 nm wavelength, while control cells to which TPPS was not added did not show any fluorescence.

Bottom Line: TPPS was effectively used to analyse two different bacteria: Pseudomonas aeruginosa and Bacillus cereus.The variation in brightness with varying concentrations of TPPS was studied.The patterns of variations for these bacteria were found to be the same, but with consistently higher brightness for Bacillus cereus.

View Article: PubMed Central - PubMed

Affiliation: Center for Biotechnology, Anna University, Chennai 600025, India.

ABSTRACT
The use of tetraammonium tetrakis(4-sulphonato)phenyl porphyrin (TPPS), a water-soluble anionic compound, as a stain to analyse bacterial cells using fluorescent microscopy was investigated. TPPS was effectively used to analyse two different bacteria: Pseudomonas aeruginosa and Bacillus cereus. The variation in brightness with varying concentrations of TPPS was studied. The patterns of variations for these bacteria were found to be the same, but with consistently higher brightness for Bacillus cereus.

No MeSH data available.