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Canine leishmaniasis in Brazil: serological follow-up of a dog population in an endemic area of american visceral leishmaniasis.

da Silva AV, de Paula AA, de Pita Pereira D, Brazil RP, Carreira JC - J Parasitol Res (2010)

Bottom Line: We performed a serological, clinical, and parasitological follow-up of a dog population in an endemic area of American Visceral Leishmaniasis estimated by indirect immunofluorescent assay (IFA) and western blot (WB).After twelve months, the results obtained from IFA demonstrated that 50% were seropositive and two serological profiles were observed: the first one ranging from 1/40 to 1/80 and the second >/=1/160.They also corroborate the high sensitivity and specificity of western blot in the diagnosis of canine leishmaniasis, suggesting the possibility of its association with IFA.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Protozoologia, FIOCRUZ, Rio de Janeiro, CEP 21040-360, Brazil.

ABSTRACT
We performed a serological, clinical, and parasitological follow-up of a dog population in an endemic area of American Visceral Leishmaniasis estimated by indirect immunofluorescent assay (IFA) and western blot (WB). After twelve months, the results obtained from IFA demonstrated that 50% were seropositive and two serological profiles were observed: the first one ranging from 1/40 to 1/80 and the second >/=1/160. By WB, it was observed that the same percentage and sera from positive dogs presented the recognition of the peptides of 29 and 32 kDa up to 8 months before IFA serum conversion. Among the positive dogs, all the sera from symptomatic ones with tissue parasitism recognized the peptide of 68.5 kDa. Our results suggest the need of modifications in the control measures regarding the elimination of the dogs. They also corroborate the high sensitivity and specificity of western blot in the diagnosis of canine leishmaniasis, suggesting the possibility of its association with IFA.

No MeSH data available.


Related in: MedlinePlus

Antigenic recognition by western blot from sera of symptomatic and asymptomatic dogs with L. (i.) chagasi. Antigen recognition of Leishmania (i.) chagasi by serum from naturally infected dogs. Lane (−): serum from serumnegative (IFA) dog. Lane (+): serum from serumpositive (IFA 1/1280) dog. Lanes a and b: IFA seropositive symptomatic dogs. Lanes c, d, and e: IFA seropositive asymptomatic dogs.
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fig2: Antigenic recognition by western blot from sera of symptomatic and asymptomatic dogs with L. (i.) chagasi. Antigen recognition of Leishmania (i.) chagasi by serum from naturally infected dogs. Lane (−): serum from serumnegative (IFA) dog. Lane (+): serum from serumpositive (IFA 1/1280) dog. Lanes a and b: IFA seropositive symptomatic dogs. Lanes c, d, and e: IFA seropositive asymptomatic dogs.

Mentions: It was observed a high seroprevalence (50%) with a strong and homogeneous pattern of antigenic recognition. The recognized polypeptide fractions were in the range of 4 to 220 kDa and all animals with confirmed infection systematically recognized peptide bands of 29 and 32 kDa (Figure 1). Only the sera from the 5 symptomatic dogs recognized the band of 68.5 besides those of 29 and 32 (Figures 1 and 2) and all those animals presented serological titers ≥1/320.


Canine leishmaniasis in Brazil: serological follow-up of a dog population in an endemic area of american visceral leishmaniasis.

da Silva AV, de Paula AA, de Pita Pereira D, Brazil RP, Carreira JC - J Parasitol Res (2010)

Antigenic recognition by western blot from sera of symptomatic and asymptomatic dogs with L. (i.) chagasi. Antigen recognition of Leishmania (i.) chagasi by serum from naturally infected dogs. Lane (−): serum from serumnegative (IFA) dog. Lane (+): serum from serumpositive (IFA 1/1280) dog. Lanes a and b: IFA seropositive symptomatic dogs. Lanes c, d, and e: IFA seropositive asymptomatic dogs.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2925097&req=5

fig2: Antigenic recognition by western blot from sera of symptomatic and asymptomatic dogs with L. (i.) chagasi. Antigen recognition of Leishmania (i.) chagasi by serum from naturally infected dogs. Lane (−): serum from serumnegative (IFA) dog. Lane (+): serum from serumpositive (IFA 1/1280) dog. Lanes a and b: IFA seropositive symptomatic dogs. Lanes c, d, and e: IFA seropositive asymptomatic dogs.
Mentions: It was observed a high seroprevalence (50%) with a strong and homogeneous pattern of antigenic recognition. The recognized polypeptide fractions were in the range of 4 to 220 kDa and all animals with confirmed infection systematically recognized peptide bands of 29 and 32 kDa (Figure 1). Only the sera from the 5 symptomatic dogs recognized the band of 68.5 besides those of 29 and 32 (Figures 1 and 2) and all those animals presented serological titers ≥1/320.

Bottom Line: We performed a serological, clinical, and parasitological follow-up of a dog population in an endemic area of American Visceral Leishmaniasis estimated by indirect immunofluorescent assay (IFA) and western blot (WB).After twelve months, the results obtained from IFA demonstrated that 50% were seropositive and two serological profiles were observed: the first one ranging from 1/40 to 1/80 and the second >/=1/160.They also corroborate the high sensitivity and specificity of western blot in the diagnosis of canine leishmaniasis, suggesting the possibility of its association with IFA.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Protozoologia, FIOCRUZ, Rio de Janeiro, CEP 21040-360, Brazil.

ABSTRACT
We performed a serological, clinical, and parasitological follow-up of a dog population in an endemic area of American Visceral Leishmaniasis estimated by indirect immunofluorescent assay (IFA) and western blot (WB). After twelve months, the results obtained from IFA demonstrated that 50% were seropositive and two serological profiles were observed: the first one ranging from 1/40 to 1/80 and the second >/=1/160. By WB, it was observed that the same percentage and sera from positive dogs presented the recognition of the peptides of 29 and 32 kDa up to 8 months before IFA serum conversion. Among the positive dogs, all the sera from symptomatic ones with tissue parasitism recognized the peptide of 68.5 kDa. Our results suggest the need of modifications in the control measures regarding the elimination of the dogs. They also corroborate the high sensitivity and specificity of western blot in the diagnosis of canine leishmaniasis, suggesting the possibility of its association with IFA.

No MeSH data available.


Related in: MedlinePlus