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A comparative study of salt tolerance parameters in 11 wild relatives of Arabidopsis thaliana.

Orsini F, D'Urzo MP, Inan G, Serra S, Oh DH, Mickelbart MV, Consiglio F, Li X, Jeong JC, Yun DJ, Bohnert HJ, Bressan RA, Maggio A - J. Exp. Bot. (2010)

Bottom Line: In a search for candidates to begin to understand, through genetic analyses, the biological bases of salt tolerance, 11 wild relatives of Arabidopsis thaliana were compared: Barbarea verna, Capsella bursa-pastoris, Hirschfeldia incana, Lepidium densiflorum, Malcolmia triloba, Lepidium virginicum, Descurainia pinnata, Sisymbrium officinale, Thellungiella parvula, Thellungiella salsuginea (previously T. halophila), and Thlaspi arvense.Only T. parvula revealed a true halophytic habitus, comparable to the better studied Thellungiella salsuginea.Major differences in growth, water transport properties, and ion accumulation are observed and discussed to describe the distinctive traits and physiological responses that can now be studied genetically in salt stress research.

View Article: PubMed Central - PubMed

Affiliation: Department of Agro-environmental Sciences and Technologies, University of Bologna, Viale Fanin 44, I-40127 Bologna, Italy.

ABSTRACT
Salinity is an abiotic stress that limits both yield and the expansion of agricultural crops to new areas. In the last 20 years our basic understanding of the mechanisms underlying plant tolerance and adaptation to saline environments has greatly improved owing to active development of advanced tools in molecular, genomics, and bioinformatics analyses. However, the full potential of investigative power has not been fully exploited, because the use of halophytes as model systems in plant salt tolerance research is largely neglected. The recent introduction of halophytic Arabidopsis-Relative Model Species (ARMS) has begun to compare and relate several unique genetic resources to the well-developed Arabidopsis model. In a search for candidates to begin to understand, through genetic analyses, the biological bases of salt tolerance, 11 wild relatives of Arabidopsis thaliana were compared: Barbarea verna, Capsella bursa-pastoris, Hirschfeldia incana, Lepidium densiflorum, Malcolmia triloba, Lepidium virginicum, Descurainia pinnata, Sisymbrium officinale, Thellungiella parvula, Thellungiella salsuginea (previously T. halophila), and Thlaspi arvense. Among these species, highly salt-tolerant (L. densiflorum and L. virginicum) and moderately salt-tolerant (M. triloba and H. incana) species were identified. Only T. parvula revealed a true halophytic habitus, comparable to the better studied Thellungiella salsuginea. Major differences in growth, water transport properties, and ion accumulation are observed and discussed to describe the distinctive traits and physiological responses that can now be studied genetically in salt stress research.

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Effect of 300 mM NaCl stress on root elongation of selected Brassicaceae species. Five days after germination on regular MS medium, with plates positioned vertically, seedlings were moved to salt. The plates were then rotated 180° to visualize further growth (root bending assay). Measurements were assessed after 10 d on 10 plants per species, with three replicates. Values are expressed as a percentage (%) of growth measured in non-salinized plants. Values are means ±SE (n=30).
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fig4: Effect of 300 mM NaCl stress on root elongation of selected Brassicaceae species. Five days after germination on regular MS medium, with plates positioned vertically, seedlings were moved to salt. The plates were then rotated 180° to visualize further growth (root bending assay). Measurements were assessed after 10 d on 10 plants per species, with three replicates. Values are expressed as a percentage (%) of growth measured in non-salinized plants. Values are means ±SE (n=30).

Mentions: The results of this and subsequent sections refer to the three selected novel ARMS, L. virginicum, D. pinnata, and T. parvula, and the two controls, A. thaliana and T. salsuginea. To confirm the growth performance under saline conditions, root growth was assessed by the root bending essay (Verslues et al., 2006). At 300 mM NaCl the growth of A. thaliana had stopped, whereas slight further growth was observed in D. pinnata. Growth rates comparable to T. salsuginea were observed in T. parvula and L. virginicum (Fig. 4).


A comparative study of salt tolerance parameters in 11 wild relatives of Arabidopsis thaliana.

Orsini F, D'Urzo MP, Inan G, Serra S, Oh DH, Mickelbart MV, Consiglio F, Li X, Jeong JC, Yun DJ, Bohnert HJ, Bressan RA, Maggio A - J. Exp. Bot. (2010)

Effect of 300 mM NaCl stress on root elongation of selected Brassicaceae species. Five days after germination on regular MS medium, with plates positioned vertically, seedlings were moved to salt. The plates were then rotated 180° to visualize further growth (root bending assay). Measurements were assessed after 10 d on 10 plants per species, with three replicates. Values are expressed as a percentage (%) of growth measured in non-salinized plants. Values are means ±SE (n=30).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2921208&req=5

fig4: Effect of 300 mM NaCl stress on root elongation of selected Brassicaceae species. Five days after germination on regular MS medium, with plates positioned vertically, seedlings were moved to salt. The plates were then rotated 180° to visualize further growth (root bending assay). Measurements were assessed after 10 d on 10 plants per species, with three replicates. Values are expressed as a percentage (%) of growth measured in non-salinized plants. Values are means ±SE (n=30).
Mentions: The results of this and subsequent sections refer to the three selected novel ARMS, L. virginicum, D. pinnata, and T. parvula, and the two controls, A. thaliana and T. salsuginea. To confirm the growth performance under saline conditions, root growth was assessed by the root bending essay (Verslues et al., 2006). At 300 mM NaCl the growth of A. thaliana had stopped, whereas slight further growth was observed in D. pinnata. Growth rates comparable to T. salsuginea were observed in T. parvula and L. virginicum (Fig. 4).

Bottom Line: In a search for candidates to begin to understand, through genetic analyses, the biological bases of salt tolerance, 11 wild relatives of Arabidopsis thaliana were compared: Barbarea verna, Capsella bursa-pastoris, Hirschfeldia incana, Lepidium densiflorum, Malcolmia triloba, Lepidium virginicum, Descurainia pinnata, Sisymbrium officinale, Thellungiella parvula, Thellungiella salsuginea (previously T. halophila), and Thlaspi arvense.Only T. parvula revealed a true halophytic habitus, comparable to the better studied Thellungiella salsuginea.Major differences in growth, water transport properties, and ion accumulation are observed and discussed to describe the distinctive traits and physiological responses that can now be studied genetically in salt stress research.

View Article: PubMed Central - PubMed

Affiliation: Department of Agro-environmental Sciences and Technologies, University of Bologna, Viale Fanin 44, I-40127 Bologna, Italy.

ABSTRACT
Salinity is an abiotic stress that limits both yield and the expansion of agricultural crops to new areas. In the last 20 years our basic understanding of the mechanisms underlying plant tolerance and adaptation to saline environments has greatly improved owing to active development of advanced tools in molecular, genomics, and bioinformatics analyses. However, the full potential of investigative power has not been fully exploited, because the use of halophytes as model systems in plant salt tolerance research is largely neglected. The recent introduction of halophytic Arabidopsis-Relative Model Species (ARMS) has begun to compare and relate several unique genetic resources to the well-developed Arabidopsis model. In a search for candidates to begin to understand, through genetic analyses, the biological bases of salt tolerance, 11 wild relatives of Arabidopsis thaliana were compared: Barbarea verna, Capsella bursa-pastoris, Hirschfeldia incana, Lepidium densiflorum, Malcolmia triloba, Lepidium virginicum, Descurainia pinnata, Sisymbrium officinale, Thellungiella parvula, Thellungiella salsuginea (previously T. halophila), and Thlaspi arvense. Among these species, highly salt-tolerant (L. densiflorum and L. virginicum) and moderately salt-tolerant (M. triloba and H. incana) species were identified. Only T. parvula revealed a true halophytic habitus, comparable to the better studied Thellungiella salsuginea. Major differences in growth, water transport properties, and ion accumulation are observed and discussed to describe the distinctive traits and physiological responses that can now be studied genetically in salt stress research.

Show MeSH