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An L1 box binding protein, GbML1, interacts with GbMYB25 to control cotton fibre development.

Zhang F, Zuo K, Zhang J, Liu X, Zhang L, Sun X, Tang K - J. Exp. Bot. (2010)

Bottom Line: GbML1 overexpression in Arabidopsis increased the number of trichomes on stems and leaves and increased the accumulation of anthocyanin in leaves.Taken together, the L1 box binding protein, GbML1 was identified as the first partner for GbMYB25 and the role of START domain was discovered to be a protein binding domain in plants.Our findings will help the improvement of cotton fibre production and the understanding of the key role of HD-Zip family and MYB family in plants.

View Article: PubMed Central - PubMed

Affiliation: Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.

ABSTRACT
Transcription factors play key roles in plant development through their interaction with cis-elements and/or other transcription factors. A HD-Zip IV family transcription factor, Gossypium barbadense Meristem Layer 1 (GbML1) has been identified and characterized here. GbML1 specifically bound to the L1 box and the promoters of GbML1 and GbRDL1. GbML1 physically interacted with a key regulator of cotton fibre development, GbMYB25. Truncated and point mutation assays indicated the START-SAD domain was required for the binding to the C terminal domain (CTD) of GbMYB25. GbML1 overexpression in Arabidopsis increased the number of trichomes on stems and leaves and increased the accumulation of anthocyanin in leaves. Taken together, the L1 box binding protein, GbML1 was identified as the first partner for GbMYB25 and the role of START domain was discovered to be a protein binding domain in plants. Our findings will help the improvement of cotton fibre production and the understanding of the key role of HD-Zip family and MYB family in plants.

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Phenotypes of overexpressing GbML1 in Arabidopsis plants. (A) Developmental phenotypes of Type I plants. 1, 25-d-old plant. 2, More trichomes on stems. 3, More trichomes on cauline leaves. (B) Developmental phenotypes of Type II plants. 1, Type II plants accumulate more anthocyanin on cotyledons. 2–4, Anthocyanin accumulation in rosette leaves and cauline leaves. (C) Expression of GbML1 and HPT transgenes and AtMYB75, ANL2, HDG12, PDF1, and RD22 endogenous genes are also shown. Tubulin (Tub) is used as a control for normalization.
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fig7: Phenotypes of overexpressing GbML1 in Arabidopsis plants. (A) Developmental phenotypes of Type I plants. 1, 25-d-old plant. 2, More trichomes on stems. 3, More trichomes on cauline leaves. (B) Developmental phenotypes of Type II plants. 1, Type II plants accumulate more anthocyanin on cotyledons. 2–4, Anthocyanin accumulation in rosette leaves and cauline leaves. (C) Expression of GbML1 and HPT transgenes and AtMYB75, ANL2, HDG12, PDF1, and RD22 endogenous genes are also shown. Tubulin (Tub) is used as a control for normalization.

Mentions: The full-length GbML1 ORF was driven under the double 35S promoter to make the overexpression construct which was later introduced into Arabidopsis. Eighteen independent GbML1 overexpression lines were obtained. In these lines, there were mainly two types of plant based on the severity of the developmental phenotypes: the first type of plant (Type I, 12/18) was developmentally similar as the plants transformed with the empty vector (CK) except that there were more trichomes on the stems and cauline leaves (Fig. 7A, 1, 2, 3). The second type of plant (Type II, 6/18) had more anthocyanin accumulation in the cotyledons (Fig. 7B, 1) and leaves (Fig. 7B, 2, 3, 4). Expression patterns of several genes related to the phenotypes were examined in Type I and Type II plants (Fig. 7C). GbML1 was highly expressed in Type II seedlings while there was only a moderate expression level of GbML1 in Type I seedlings. Genes related to anthacynin biosynthesis including AtMYB75 (Teng et al., 2005) and ANL2 (Kobo et al., 1999) were induced in the transgenic plants compared with the CK. HDG12 was also induced in GbML1 overexpressing plants. The expression level of RD22, PDF1, and the selective antibiotic gene HPT (hygromycin phosphotransferase) were similar to the CK in Type I and Type II overexpressing plants.


An L1 box binding protein, GbML1, interacts with GbMYB25 to control cotton fibre development.

Zhang F, Zuo K, Zhang J, Liu X, Zhang L, Sun X, Tang K - J. Exp. Bot. (2010)

Phenotypes of overexpressing GbML1 in Arabidopsis plants. (A) Developmental phenotypes of Type I plants. 1, 25-d-old plant. 2, More trichomes on stems. 3, More trichomes on cauline leaves. (B) Developmental phenotypes of Type II plants. 1, Type II plants accumulate more anthocyanin on cotyledons. 2–4, Anthocyanin accumulation in rosette leaves and cauline leaves. (C) Expression of GbML1 and HPT transgenes and AtMYB75, ANL2, HDG12, PDF1, and RD22 endogenous genes are also shown. Tubulin (Tub) is used as a control for normalization.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2921199&req=5

fig7: Phenotypes of overexpressing GbML1 in Arabidopsis plants. (A) Developmental phenotypes of Type I plants. 1, 25-d-old plant. 2, More trichomes on stems. 3, More trichomes on cauline leaves. (B) Developmental phenotypes of Type II plants. 1, Type II plants accumulate more anthocyanin on cotyledons. 2–4, Anthocyanin accumulation in rosette leaves and cauline leaves. (C) Expression of GbML1 and HPT transgenes and AtMYB75, ANL2, HDG12, PDF1, and RD22 endogenous genes are also shown. Tubulin (Tub) is used as a control for normalization.
Mentions: The full-length GbML1 ORF was driven under the double 35S promoter to make the overexpression construct which was later introduced into Arabidopsis. Eighteen independent GbML1 overexpression lines were obtained. In these lines, there were mainly two types of plant based on the severity of the developmental phenotypes: the first type of plant (Type I, 12/18) was developmentally similar as the plants transformed with the empty vector (CK) except that there were more trichomes on the stems and cauline leaves (Fig. 7A, 1, 2, 3). The second type of plant (Type II, 6/18) had more anthocyanin accumulation in the cotyledons (Fig. 7B, 1) and leaves (Fig. 7B, 2, 3, 4). Expression patterns of several genes related to the phenotypes were examined in Type I and Type II plants (Fig. 7C). GbML1 was highly expressed in Type II seedlings while there was only a moderate expression level of GbML1 in Type I seedlings. Genes related to anthacynin biosynthesis including AtMYB75 (Teng et al., 2005) and ANL2 (Kobo et al., 1999) were induced in the transgenic plants compared with the CK. HDG12 was also induced in GbML1 overexpressing plants. The expression level of RD22, PDF1, and the selective antibiotic gene HPT (hygromycin phosphotransferase) were similar to the CK in Type I and Type II overexpressing plants.

Bottom Line: GbML1 overexpression in Arabidopsis increased the number of trichomes on stems and leaves and increased the accumulation of anthocyanin in leaves.Taken together, the L1 box binding protein, GbML1 was identified as the first partner for GbMYB25 and the role of START domain was discovered to be a protein binding domain in plants.Our findings will help the improvement of cotton fibre production and the understanding of the key role of HD-Zip family and MYB family in plants.

View Article: PubMed Central - PubMed

Affiliation: Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.

ABSTRACT
Transcription factors play key roles in plant development through their interaction with cis-elements and/or other transcription factors. A HD-Zip IV family transcription factor, Gossypium barbadense Meristem Layer 1 (GbML1) has been identified and characterized here. GbML1 specifically bound to the L1 box and the promoters of GbML1 and GbRDL1. GbML1 physically interacted with a key regulator of cotton fibre development, GbMYB25. Truncated and point mutation assays indicated the START-SAD domain was required for the binding to the C terminal domain (CTD) of GbMYB25. GbML1 overexpression in Arabidopsis increased the number of trichomes on stems and leaves and increased the accumulation of anthocyanin in leaves. Taken together, the L1 box binding protein, GbML1 was identified as the first partner for GbMYB25 and the role of START domain was discovered to be a protein binding domain in plants. Our findings will help the improvement of cotton fibre production and the understanding of the key role of HD-Zip family and MYB family in plants.

Show MeSH