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An L1 box binding protein, GbML1, interacts with GbMYB25 to control cotton fibre development.

Zhang F, Zuo K, Zhang J, Liu X, Zhang L, Sun X, Tang K - J. Exp. Bot. (2010)

Bottom Line: GbML1 overexpression in Arabidopsis increased the number of trichomes on stems and leaves and increased the accumulation of anthocyanin in leaves.Taken together, the L1 box binding protein, GbML1 was identified as the first partner for GbMYB25 and the role of START domain was discovered to be a protein binding domain in plants.Our findings will help the improvement of cotton fibre production and the understanding of the key role of HD-Zip family and MYB family in plants.

View Article: PubMed Central - PubMed

Affiliation: Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.

ABSTRACT
Transcription factors play key roles in plant development through their interaction with cis-elements and/or other transcription factors. A HD-Zip IV family transcription factor, Gossypium barbadense Meristem Layer 1 (GbML1) has been identified and characterized here. GbML1 specifically bound to the L1 box and the promoters of GbML1 and GbRDL1. GbML1 physically interacted with a key regulator of cotton fibre development, GbMYB25. Truncated and point mutation assays indicated the START-SAD domain was required for the binding to the C terminal domain (CTD) of GbMYB25. GbML1 overexpression in Arabidopsis increased the number of trichomes on stems and leaves and increased the accumulation of anthocyanin in leaves. Taken together, the L1 box binding protein, GbML1 was identified as the first partner for GbMYB25 and the role of START domain was discovered to be a protein binding domain in plants. Our findings will help the improvement of cotton fibre production and the understanding of the key role of HD-Zip family and MYB family in plants.

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GbML1 binds to the C terminal domain of GbMYB25. (Left) Diagram of different preys used in mapping the interaction part of GbMYB25. CTD, C terminal domain including C1 and C2. C1 domain is a conserved region other than the MYB domain among MIXTA type proteins. (Right) Y2H assay was carried out as in Fig. 4B. The yeast two-hybrid assay indicates that the CTD of GbMYB25 binds to GbML1 while the MYB repeats do not. Both C1 and C2 domains of GbMYB25 are required for the binding to GbML1.
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fig6: GbML1 binds to the C terminal domain of GbMYB25. (Left) Diagram of different preys used in mapping the interaction part of GbMYB25. CTD, C terminal domain including C1 and C2. C1 domain is a conserved region other than the MYB domain among MIXTA type proteins. (Right) Y2H assay was carried out as in Fig. 4B. The yeast two-hybrid assay indicates that the CTD of GbMYB25 binds to GbML1 while the MYB repeats do not. Both C1 and C2 domains of GbMYB25 are required for the binding to GbML1.

Mentions: The protein–protein interaction domain of GbMYB25 (Fig. 6) was also investigated. The C terminal part (aa: 113–309) interacted with GbML1 as strongly as the complete GbMYB25. The N terminal part of GbMYB25 (aa: 1–112) containing the R2R3-MYB repeats could not interact with GbML1. Neither the C1 part (aa: 113–170) nor the C2 part (aa: 171–309) interacted with GbML1, suggesting both parts were needed for the interaction.


An L1 box binding protein, GbML1, interacts with GbMYB25 to control cotton fibre development.

Zhang F, Zuo K, Zhang J, Liu X, Zhang L, Sun X, Tang K - J. Exp. Bot. (2010)

GbML1 binds to the C terminal domain of GbMYB25. (Left) Diagram of different preys used in mapping the interaction part of GbMYB25. CTD, C terminal domain including C1 and C2. C1 domain is a conserved region other than the MYB domain among MIXTA type proteins. (Right) Y2H assay was carried out as in Fig. 4B. The yeast two-hybrid assay indicates that the CTD of GbMYB25 binds to GbML1 while the MYB repeats do not. Both C1 and C2 domains of GbMYB25 are required for the binding to GbML1.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2921199&req=5

fig6: GbML1 binds to the C terminal domain of GbMYB25. (Left) Diagram of different preys used in mapping the interaction part of GbMYB25. CTD, C terminal domain including C1 and C2. C1 domain is a conserved region other than the MYB domain among MIXTA type proteins. (Right) Y2H assay was carried out as in Fig. 4B. The yeast two-hybrid assay indicates that the CTD of GbMYB25 binds to GbML1 while the MYB repeats do not. Both C1 and C2 domains of GbMYB25 are required for the binding to GbML1.
Mentions: The protein–protein interaction domain of GbMYB25 (Fig. 6) was also investigated. The C terminal part (aa: 113–309) interacted with GbML1 as strongly as the complete GbMYB25. The N terminal part of GbMYB25 (aa: 1–112) containing the R2R3-MYB repeats could not interact with GbML1. Neither the C1 part (aa: 113–170) nor the C2 part (aa: 171–309) interacted with GbML1, suggesting both parts were needed for the interaction.

Bottom Line: GbML1 overexpression in Arabidopsis increased the number of trichomes on stems and leaves and increased the accumulation of anthocyanin in leaves.Taken together, the L1 box binding protein, GbML1 was identified as the first partner for GbMYB25 and the role of START domain was discovered to be a protein binding domain in plants.Our findings will help the improvement of cotton fibre production and the understanding of the key role of HD-Zip family and MYB family in plants.

View Article: PubMed Central - PubMed

Affiliation: Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.

ABSTRACT
Transcription factors play key roles in plant development through their interaction with cis-elements and/or other transcription factors. A HD-Zip IV family transcription factor, Gossypium barbadense Meristem Layer 1 (GbML1) has been identified and characterized here. GbML1 specifically bound to the L1 box and the promoters of GbML1 and GbRDL1. GbML1 physically interacted with a key regulator of cotton fibre development, GbMYB25. Truncated and point mutation assays indicated the START-SAD domain was required for the binding to the C terminal domain (CTD) of GbMYB25. GbML1 overexpression in Arabidopsis increased the number of trichomes on stems and leaves and increased the accumulation of anthocyanin in leaves. Taken together, the L1 box binding protein, GbML1 was identified as the first partner for GbMYB25 and the role of START domain was discovered to be a protein binding domain in plants. Our findings will help the improvement of cotton fibre production and the understanding of the key role of HD-Zip family and MYB family in plants.

Show MeSH