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First case of detection of Plasmodium knowlesi in Spain by Real Time PCR in a traveller from Southeast Asia.

Ta TT, Salas A, Ali-Tammam M, Martínez Mdel C, Lanza M, Arroyo E, Rubio JM - Malar. J. (2010)

Bottom Line: Previously, Plasmodium knowlesi was not considered as a species of Plasmodium that could cause malaria in human beings, as it is parasite of long-tailed (Macaca fascicularis) and pig-tailed (Macaca nemestrina) macaques found in Southeast Asia.This patient was discharged and asymptomatic when the positive result to P. knowlesi was reported.Prior to this case, there have been two more reports of European travellers with malaria caused by P. knowlesi, a Finnish man who travelled to Peninsular Malaysia during four weeks in March 2007, and a Swedish man who did a short visit to Malaysian Borneo in October 2006.

View Article: PubMed Central - HTML - PubMed

Affiliation: Malaria & Emerging Parasitic Diseases Laboratory, Parasitology Department, National Centre of Microbiology, Instituto de Salud Carlos III, Cra, Majadahonda Pozuelo Km, 2, Majadahonda, 28220 Madrid, Spain.

ABSTRACT
Previously, Plasmodium knowlesi was not considered as a species of Plasmodium that could cause malaria in human beings, as it is parasite of long-tailed (Macaca fascicularis) and pig-tailed (Macaca nemestrina) macaques found in Southeast Asia. A case of infection by P. knowlesi is described in a Spanish traveller, who came back to Spain with daily fever after his last overseas travel, which was a six-month holiday in forested areas of Southeast Asia between 2008 and 2009. His P. knowlesi infection was detected by multiplex Real time quantitative PCR and confirmed by sequencing the amplified fragment. Using nested multiplex malaria PCR (reference method in Spain) and a rapid diagnostic test, the P. knowlesi infection was negative. This patient was discharged and asymptomatic when the positive result to P. knowlesi was reported. Prior to this case, there have been two more reports of European travellers with malaria caused by P. knowlesi, a Finnish man who travelled to Peninsular Malaysia during four weeks in March 2007, and a Swedish man who did a short visit to Malaysian Borneo in October 2006. Taken together with this report of P. knowlesi infection in a Spanish traveller returning from Southeast Asia, this is the third case of P. knowlesi infection in Europe, indicating that this simian parasite can infect visitors to endemic areas in Southeast Asia. This last European case is quite surprising, given that it is an untreated-symptomatic P. knowlesi in human, in contrast to what is currently known about P. knowlesi infection. Most previous reports of human P. knowlesi malaria infections were in adults, often with symptoms and relatively high parasite densities, up to the recent report in Ninh Thuan province, located in the southern part of central Vietnam, inhabited mainly by the Ra-glai ethnic minority, in which all P. knowlesi infections were asymptomatic, co-infected with P. malariae, with low parasite densities and two of the three identified cases were very young children under five years old.

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Giemsa-stained thin blood films of the Spanish traveller infected with multiplex Real Time quantitative PCR-confirmed Plasmodium knowlesi. By microscopy, inside the infected erythrocytes, structures compatible with Plasmodium can be appreciated. a-b: mature trophozoites; c: gametocyte; d: indefinite stage; e-f: early trophozoites.
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Figure 2: Giemsa-stained thin blood films of the Spanish traveller infected with multiplex Real Time quantitative PCR-confirmed Plasmodium knowlesi. By microscopy, inside the infected erythrocytes, structures compatible with Plasmodium can be appreciated. a-b: mature trophozoites; c: gametocyte; d: indefinite stage; e-f: early trophozoites.

Mentions: After this amazing finding in this sample, a thorough analysis was performed on it. In a rapid diagnostic test for malaria (Binax Now Malaria Test; Binax, Inc., USA), the sample was both negative for P. falciparum histidine-rich protein 2 and for pan-malarial aldolase antigen, suggesting a non-Plasmodium infection. Retrospective examination of Giemsa-stained thin blood films showed infected erythrocytes with an inconclusive morphologic appearance. The parasite structure found inside the erythrocytes was compatible with Plasmodium which suggested a possible infection by Plasmodium, in agreement with multiplex Real time quantitative PCR results (Figure 2). The serodiagnosis of malaria caused by P. falciparum was negative (Falciparum-Spot IF, Biomérieux S.A., France).


First case of detection of Plasmodium knowlesi in Spain by Real Time PCR in a traveller from Southeast Asia.

Ta TT, Salas A, Ali-Tammam M, Martínez Mdel C, Lanza M, Arroyo E, Rubio JM - Malar. J. (2010)

Giemsa-stained thin blood films of the Spanish traveller infected with multiplex Real Time quantitative PCR-confirmed Plasmodium knowlesi. By microscopy, inside the infected erythrocytes, structures compatible with Plasmodium can be appreciated. a-b: mature trophozoites; c: gametocyte; d: indefinite stage; e-f: early trophozoites.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2921078&req=5

Figure 2: Giemsa-stained thin blood films of the Spanish traveller infected with multiplex Real Time quantitative PCR-confirmed Plasmodium knowlesi. By microscopy, inside the infected erythrocytes, structures compatible with Plasmodium can be appreciated. a-b: mature trophozoites; c: gametocyte; d: indefinite stage; e-f: early trophozoites.
Mentions: After this amazing finding in this sample, a thorough analysis was performed on it. In a rapid diagnostic test for malaria (Binax Now Malaria Test; Binax, Inc., USA), the sample was both negative for P. falciparum histidine-rich protein 2 and for pan-malarial aldolase antigen, suggesting a non-Plasmodium infection. Retrospective examination of Giemsa-stained thin blood films showed infected erythrocytes with an inconclusive morphologic appearance. The parasite structure found inside the erythrocytes was compatible with Plasmodium which suggested a possible infection by Plasmodium, in agreement with multiplex Real time quantitative PCR results (Figure 2). The serodiagnosis of malaria caused by P. falciparum was negative (Falciparum-Spot IF, Biomérieux S.A., France).

Bottom Line: Previously, Plasmodium knowlesi was not considered as a species of Plasmodium that could cause malaria in human beings, as it is parasite of long-tailed (Macaca fascicularis) and pig-tailed (Macaca nemestrina) macaques found in Southeast Asia.This patient was discharged and asymptomatic when the positive result to P. knowlesi was reported.Prior to this case, there have been two more reports of European travellers with malaria caused by P. knowlesi, a Finnish man who travelled to Peninsular Malaysia during four weeks in March 2007, and a Swedish man who did a short visit to Malaysian Borneo in October 2006.

View Article: PubMed Central - HTML - PubMed

Affiliation: Malaria & Emerging Parasitic Diseases Laboratory, Parasitology Department, National Centre of Microbiology, Instituto de Salud Carlos III, Cra, Majadahonda Pozuelo Km, 2, Majadahonda, 28220 Madrid, Spain.

ABSTRACT
Previously, Plasmodium knowlesi was not considered as a species of Plasmodium that could cause malaria in human beings, as it is parasite of long-tailed (Macaca fascicularis) and pig-tailed (Macaca nemestrina) macaques found in Southeast Asia. A case of infection by P. knowlesi is described in a Spanish traveller, who came back to Spain with daily fever after his last overseas travel, which was a six-month holiday in forested areas of Southeast Asia between 2008 and 2009. His P. knowlesi infection was detected by multiplex Real time quantitative PCR and confirmed by sequencing the amplified fragment. Using nested multiplex malaria PCR (reference method in Spain) and a rapid diagnostic test, the P. knowlesi infection was negative. This patient was discharged and asymptomatic when the positive result to P. knowlesi was reported. Prior to this case, there have been two more reports of European travellers with malaria caused by P. knowlesi, a Finnish man who travelled to Peninsular Malaysia during four weeks in March 2007, and a Swedish man who did a short visit to Malaysian Borneo in October 2006. Taken together with this report of P. knowlesi infection in a Spanish traveller returning from Southeast Asia, this is the third case of P. knowlesi infection in Europe, indicating that this simian parasite can infect visitors to endemic areas in Southeast Asia. This last European case is quite surprising, given that it is an untreated-symptomatic P. knowlesi in human, in contrast to what is currently known about P. knowlesi infection. Most previous reports of human P. knowlesi malaria infections were in adults, often with symptoms and relatively high parasite densities, up to the recent report in Ninh Thuan province, located in the southern part of central Vietnam, inhabited mainly by the Ra-glai ethnic minority, in which all P. knowlesi infections were asymptomatic, co-infected with P. malariae, with low parasite densities and two of the three identified cases were very young children under five years old.

Show MeSH
Related in: MedlinePlus