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Molecular imaging of angiogenesis with SPECT.

Dijkgraaf I, Boerman OC - Eur. J. Nucl. Med. Mol. Imaging (2010)

Bottom Line: Single-photon emission computed tomography (SPECT) and position emission tomography (PET) are the two main imaging modalities in nuclear medicine.Commonly used gamma emitters are: (99m)Tc (E(max) 141 keV, T (1/2) 6.02 h), (123)I (E(max) 529 keV, T (1/2) 13.0 h) and (111)In (E(max) 245 keV, T (1/2) 67.2 h).The resolution of microSPECT cameras (<0.5 mm) is higher than that of microPET cameras (>1.5 mm).

View Article: PubMed Central - PubMed

Affiliation: Department of Nuclear Medicine, Radboud University Nijmegen Medical Center, The Netherlands. I.Dijkgraaf@nucmed.umcn.nl

ABSTRACT
Single-photon emission computed tomography (SPECT) and position emission tomography (PET) are the two main imaging modalities in nuclear medicine. SPECT imaging is more widely available than PET imaging and the radionuclides used for SPECT are easier to prepare and usually have a longer half-life than those used for PET. In addition, SPECT is a less expensive technique than PET. Commonly used gamma emitters are: (99m)Tc (E(max) 141 keV, T (1/2) 6.02 h), (123)I (E(max) 529 keV, T (1/2) 13.0 h) and (111)In (E(max) 245 keV, T (1/2) 67.2 h). Compared to clinical SPECT, PET has a higher spatial resolution and the possibility to more accurately estimate the in vivo concentration of a tracer. In preclinical imaging, the situation is quite different. The resolution of microSPECT cameras (<0.5 mm) is higher than that of microPET cameras (>1.5 mm). In this report, studies on new radiolabelled tracers for SPECT imaging of angiogenesis in tumours are reviewed.

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Related in: MedlinePlus

a Biodistribution of 111In-DOTA-E-c(RGDfK), 111In-DOTA-E-[c(RGDfK)]2 and 111In-DOTA-E{E[c(RGDfK)]2}2 at 8 h p.i. in athymic mice with s.c. SK-RC-52 tumours (5 mice/group). b Tumour to blood ratios of 111In-DOTA-E-c(RGDfK), 111In-DOTA-E-[c(RGDfK)]2 and 111In-DOTA-E{E[c(RGDfK)]2}2 at 2, 8 and 24 h after injection in athymic mice with s.c. SK-RC-52 tumours. Each bar represents the mean values ± SD. Values were analysed using one-way analysis of variance, *p < 0.05
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Fig3: a Biodistribution of 111In-DOTA-E-c(RGDfK), 111In-DOTA-E-[c(RGDfK)]2 and 111In-DOTA-E{E[c(RGDfK)]2}2 at 8 h p.i. in athymic mice with s.c. SK-RC-52 tumours (5 mice/group). b Tumour to blood ratios of 111In-DOTA-E-c(RGDfK), 111In-DOTA-E-[c(RGDfK)]2 and 111In-DOTA-E{E[c(RGDfK)]2}2 at 2, 8 and 24 h after injection in athymic mice with s.c. SK-RC-52 tumours. Each bar represents the mean values ± SD. Values were analysed using one-way analysis of variance, *p < 0.05

Mentions: The multivalency effect was further explored by the synthesis of the DOTA-conjugated tetrameric analogue E{E[c(RGDfK)]2}2 [63]. The in vitro affinity and the in vivo tumour targeting characteristics of the 111In-labelled mono-, di- and tetramer to αvβ3-expressing tumours were determined (IC50 values; monomer: 120 nM, dimer: 69.9 nM and tetramer: 19.6 nM). The tetramer showed improved tumour targeting (7.40 ± 1.12%ID/g) compared to the dimer (5.17 ± 1.22%ID/g at 8 h p.i.). Analogously, the dimer demonstrated improved tumour targeting compared to the monomer (2.30 ± 0.34%ID/g at 8 h p.i., Fig. 3).Fig. 3


Molecular imaging of angiogenesis with SPECT.

Dijkgraaf I, Boerman OC - Eur. J. Nucl. Med. Mol. Imaging (2010)

a Biodistribution of 111In-DOTA-E-c(RGDfK), 111In-DOTA-E-[c(RGDfK)]2 and 111In-DOTA-E{E[c(RGDfK)]2}2 at 8 h p.i. in athymic mice with s.c. SK-RC-52 tumours (5 mice/group). b Tumour to blood ratios of 111In-DOTA-E-c(RGDfK), 111In-DOTA-E-[c(RGDfK)]2 and 111In-DOTA-E{E[c(RGDfK)]2}2 at 2, 8 and 24 h after injection in athymic mice with s.c. SK-RC-52 tumours. Each bar represents the mean values ± SD. Values were analysed using one-way analysis of variance, *p < 0.05
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2914864&req=5

Fig3: a Biodistribution of 111In-DOTA-E-c(RGDfK), 111In-DOTA-E-[c(RGDfK)]2 and 111In-DOTA-E{E[c(RGDfK)]2}2 at 8 h p.i. in athymic mice with s.c. SK-RC-52 tumours (5 mice/group). b Tumour to blood ratios of 111In-DOTA-E-c(RGDfK), 111In-DOTA-E-[c(RGDfK)]2 and 111In-DOTA-E{E[c(RGDfK)]2}2 at 2, 8 and 24 h after injection in athymic mice with s.c. SK-RC-52 tumours. Each bar represents the mean values ± SD. Values were analysed using one-way analysis of variance, *p < 0.05
Mentions: The multivalency effect was further explored by the synthesis of the DOTA-conjugated tetrameric analogue E{E[c(RGDfK)]2}2 [63]. The in vitro affinity and the in vivo tumour targeting characteristics of the 111In-labelled mono-, di- and tetramer to αvβ3-expressing tumours were determined (IC50 values; monomer: 120 nM, dimer: 69.9 nM and tetramer: 19.6 nM). The tetramer showed improved tumour targeting (7.40 ± 1.12%ID/g) compared to the dimer (5.17 ± 1.22%ID/g at 8 h p.i.). Analogously, the dimer demonstrated improved tumour targeting compared to the monomer (2.30 ± 0.34%ID/g at 8 h p.i., Fig. 3).Fig. 3

Bottom Line: Single-photon emission computed tomography (SPECT) and position emission tomography (PET) are the two main imaging modalities in nuclear medicine.Commonly used gamma emitters are: (99m)Tc (E(max) 141 keV, T (1/2) 6.02 h), (123)I (E(max) 529 keV, T (1/2) 13.0 h) and (111)In (E(max) 245 keV, T (1/2) 67.2 h).The resolution of microSPECT cameras (<0.5 mm) is higher than that of microPET cameras (>1.5 mm).

View Article: PubMed Central - PubMed

Affiliation: Department of Nuclear Medicine, Radboud University Nijmegen Medical Center, The Netherlands. I.Dijkgraaf@nucmed.umcn.nl

ABSTRACT
Single-photon emission computed tomography (SPECT) and position emission tomography (PET) are the two main imaging modalities in nuclear medicine. SPECT imaging is more widely available than PET imaging and the radionuclides used for SPECT are easier to prepare and usually have a longer half-life than those used for PET. In addition, SPECT is a less expensive technique than PET. Commonly used gamma emitters are: (99m)Tc (E(max) 141 keV, T (1/2) 6.02 h), (123)I (E(max) 529 keV, T (1/2) 13.0 h) and (111)In (E(max) 245 keV, T (1/2) 67.2 h). Compared to clinical SPECT, PET has a higher spatial resolution and the possibility to more accurately estimate the in vivo concentration of a tracer. In preclinical imaging, the situation is quite different. The resolution of microSPECT cameras (<0.5 mm) is higher than that of microPET cameras (>1.5 mm). In this report, studies on new radiolabelled tracers for SPECT imaging of angiogenesis in tumours are reviewed.

Show MeSH
Related in: MedlinePlus