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Increased cellular uptake of biocompatible superparamagnetic iron oxide nanoparticles into malignant cells by an external magnetic field.

Prijic S, Scancar J, Romih R, Cemazar M, Bregar VB, Znidarsic A, Sersa G - J. Membr. Biol. (2010)

Bottom Line: SPIONs, dispersed in 0.9% NaCl solution, resulted in a stable ferrofluid at physiological pH for several months.Exposure to neodymium-iron-boron magnets significantly increased the cellular uptake of SPIONs, predominantly into malignant cells.Their cellular uptake was dependent on the cell type, and their accumulation within the cells was dependent on the duration of exposure to an external magnetic field.

View Article: PubMed Central - PubMed

Affiliation: Kolektor Group, Nanotesla Institute, Stegne 29, 1521, Ljubljana, Slovenia. sara.prijic@nanotesla.si

ABSTRACT
Superparamagnetic iron oxide nanoparticles (SPIONs) are used as delivery systems for different therapeutics including nucleic acids for magnetofection-mediated gene therapy. The aim of our study was to evaluate physicochemical properties, biocompatibility, cellular uptake and trafficking pathways of the custom-synthesized SPIONs for their potential use in magnetofection. Custom-synthesized SPIONs were tested for size, shape, crystalline composition and magnetic behavior using a transmission electron microscope, X-ray diffractometer and magnetometer. SPIONs were dispersed in different aqueous media to obtain ferrofluids, which were tested for pH and stability using a pH meter and zetameter. Cytotoxicity was determined using the MTS and clonogenic assays. Cellular uptake and trafficking pathways were qualitatively evaluated by transmission electron microscopy and quantitatively by inductively coupled plasma atomic emission spectrometry. SPIONs were composed of an iron oxide core with a diameter of 8-9 nm, coated with a 2-nm-thick layer of silica. SPIONs, dispersed in 0.9% NaCl solution, resulted in a stable ferrofluid at physiological pH for several months. SPIONs were not cytotoxic in a broad range of concentrations and were readily internalized into different cells by endocytosis. Exposure to neodymium-iron-boron magnets significantly increased the cellular uptake of SPIONs, predominantly into malignant cells. The prepared SPIONs displayed adequate physicochemical and biomedical properties for potential use in magnetofection. Their cellular uptake was dependent on the cell type, and their accumulation within the cells was dependent on the duration of exposure to an external magnetic field.

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Hysteresis loop of SPIONs obtained by a magnetometer. Mass magnetization and absence of magnetic behavior when not exposed to an external magnetic field indicate their suitability for use in biomedical applications. Data are presented for a representative sample
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Fig3: Hysteresis loop of SPIONs obtained by a magnetometer. Mass magnetization and absence of magnetic behavior when not exposed to an external magnetic field indicate their suitability for use in biomedical applications. Data are presented for a representative sample

Mentions: Magnetic properties of SPIONs were evaluated by a magnetometer. SPION mass magnetization as a function of an applied magnetic field at room temperature was 36.27 ± 1.45 Am2/kg. The hysteresis loop obtained demonstrated superparamagnetic behavior of SPIONs due to zero coercivity (Fig. 3). The data indicate that magnetic properties of SPIONs are favorable for their use in biomedical applications—namely, SPIONs demonstrated magnetic behavior only in the presence of an applied external magnetic field.Fig. 3


Increased cellular uptake of biocompatible superparamagnetic iron oxide nanoparticles into malignant cells by an external magnetic field.

Prijic S, Scancar J, Romih R, Cemazar M, Bregar VB, Znidarsic A, Sersa G - J. Membr. Biol. (2010)

Hysteresis loop of SPIONs obtained by a magnetometer. Mass magnetization and absence of magnetic behavior when not exposed to an external magnetic field indicate their suitability for use in biomedical applications. Data are presented for a representative sample
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2914263&req=5

Fig3: Hysteresis loop of SPIONs obtained by a magnetometer. Mass magnetization and absence of magnetic behavior when not exposed to an external magnetic field indicate their suitability for use in biomedical applications. Data are presented for a representative sample
Mentions: Magnetic properties of SPIONs were evaluated by a magnetometer. SPION mass magnetization as a function of an applied magnetic field at room temperature was 36.27 ± 1.45 Am2/kg. The hysteresis loop obtained demonstrated superparamagnetic behavior of SPIONs due to zero coercivity (Fig. 3). The data indicate that magnetic properties of SPIONs are favorable for their use in biomedical applications—namely, SPIONs demonstrated magnetic behavior only in the presence of an applied external magnetic field.Fig. 3

Bottom Line: SPIONs, dispersed in 0.9% NaCl solution, resulted in a stable ferrofluid at physiological pH for several months.Exposure to neodymium-iron-boron magnets significantly increased the cellular uptake of SPIONs, predominantly into malignant cells.Their cellular uptake was dependent on the cell type, and their accumulation within the cells was dependent on the duration of exposure to an external magnetic field.

View Article: PubMed Central - PubMed

Affiliation: Kolektor Group, Nanotesla Institute, Stegne 29, 1521, Ljubljana, Slovenia. sara.prijic@nanotesla.si

ABSTRACT
Superparamagnetic iron oxide nanoparticles (SPIONs) are used as delivery systems for different therapeutics including nucleic acids for magnetofection-mediated gene therapy. The aim of our study was to evaluate physicochemical properties, biocompatibility, cellular uptake and trafficking pathways of the custom-synthesized SPIONs for their potential use in magnetofection. Custom-synthesized SPIONs were tested for size, shape, crystalline composition and magnetic behavior using a transmission electron microscope, X-ray diffractometer and magnetometer. SPIONs were dispersed in different aqueous media to obtain ferrofluids, which were tested for pH and stability using a pH meter and zetameter. Cytotoxicity was determined using the MTS and clonogenic assays. Cellular uptake and trafficking pathways were qualitatively evaluated by transmission electron microscopy and quantitatively by inductively coupled plasma atomic emission spectrometry. SPIONs were composed of an iron oxide core with a diameter of 8-9 nm, coated with a 2-nm-thick layer of silica. SPIONs, dispersed in 0.9% NaCl solution, resulted in a stable ferrofluid at physiological pH for several months. SPIONs were not cytotoxic in a broad range of concentrations and were readily internalized into different cells by endocytosis. Exposure to neodymium-iron-boron magnets significantly increased the cellular uptake of SPIONs, predominantly into malignant cells. The prepared SPIONs displayed adequate physicochemical and biomedical properties for potential use in magnetofection. Their cellular uptake was dependent on the cell type, and their accumulation within the cells was dependent on the duration of exposure to an external magnetic field.

Show MeSH
Related in: MedlinePlus