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Metastable pores at the onset of constant-current electroporation.

Kotulska M, Basalyga J, Derylo MB, Sadowski P - J. Membr. Biol. (2010)

Bottom Line: During this stage two species of metastable pores open, the majority of very low conductance that seem not fully developed as hydrophilic electropores.Membrane capacitance has a great impact on the membrane susceptibility to the pore appearance, related to its thickness and integrity.Pores of nonperfect membranes appear more easily, but they do not live any longer than others.

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Affiliation: Institute of Biomedical Engineering and Instrumentation, Wroclaw University of Technology, ul. Wybrzeze Wyspianskiego 27, 50-370, Wroclaw, Poland. malgorzata.kotulska@pwr.wroc.pl

ABSTRACT
Single metastable nanopores, appearing before the actual electroporation under constant-current conditions, are used to characterize the onset of electroporation. Unlike the long-lived electropores typical of the current controlled methods, these pores survive for milliseconds and observing them is possible due to slow development of electroporation, provided by the gradual accumulation of charges on a planar membrane. Analysis of the metastable pore appearance frequency and lifetime shows the first introductory stage of electroporation. During this stage two species of metastable pores open, the majority of very low conductance that seem not fully developed as hydrophilic electropores. The experiments reveal that voltage value defines the electroporation onset while the current value affects the rate of electroporation. Membrane capacitance has a great impact on the membrane susceptibility to the pore appearance, related to its thickness and integrity. Pores of nonperfect membranes appear more easily, but they do not live any longer than others.

Show MeSH
Lifetime of the prepore is independent of membrane capacitance (data for I = 0.2 nA)
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Fig9: Lifetime of the prepore is independent of membrane capacitance (data for I = 0.2 nA)

Mentions: A similar study, however involving membrane capacitance, showed no dependence of the pore lifetime on membrane capacitance (Fig. 9 shows nonaveraged data for I = 0.22 nA). This may seem slightly surprising when the appearance frequency is recalled (Fig. 7), but it shows that pores opening in nonperfect membranes only appear more easily but do not tend to behave any more similar to the proper long-lived electropores. We did not observe any relation between pore lifetime and its conductance (data not shown).Fig. 9


Metastable pores at the onset of constant-current electroporation.

Kotulska M, Basalyga J, Derylo MB, Sadowski P - J. Membr. Biol. (2010)

Lifetime of the prepore is independent of membrane capacitance (data for I = 0.2 nA)
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2914259&req=5

Fig9: Lifetime of the prepore is independent of membrane capacitance (data for I = 0.2 nA)
Mentions: A similar study, however involving membrane capacitance, showed no dependence of the pore lifetime on membrane capacitance (Fig. 9 shows nonaveraged data for I = 0.22 nA). This may seem slightly surprising when the appearance frequency is recalled (Fig. 7), but it shows that pores opening in nonperfect membranes only appear more easily but do not tend to behave any more similar to the proper long-lived electropores. We did not observe any relation between pore lifetime and its conductance (data not shown).Fig. 9

Bottom Line: During this stage two species of metastable pores open, the majority of very low conductance that seem not fully developed as hydrophilic electropores.Membrane capacitance has a great impact on the membrane susceptibility to the pore appearance, related to its thickness and integrity.Pores of nonperfect membranes appear more easily, but they do not live any longer than others.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biomedical Engineering and Instrumentation, Wroclaw University of Technology, ul. Wybrzeze Wyspianskiego 27, 50-370, Wroclaw, Poland. malgorzata.kotulska@pwr.wroc.pl

ABSTRACT
Single metastable nanopores, appearing before the actual electroporation under constant-current conditions, are used to characterize the onset of electroporation. Unlike the long-lived electropores typical of the current controlled methods, these pores survive for milliseconds and observing them is possible due to slow development of electroporation, provided by the gradual accumulation of charges on a planar membrane. Analysis of the metastable pore appearance frequency and lifetime shows the first introductory stage of electroporation. During this stage two species of metastable pores open, the majority of very low conductance that seem not fully developed as hydrophilic electropores. The experiments reveal that voltage value defines the electroporation onset while the current value affects the rate of electroporation. Membrane capacitance has a great impact on the membrane susceptibility to the pore appearance, related to its thickness and integrity. Pores of nonperfect membranes appear more easily, but they do not live any longer than others.

Show MeSH