Limits...
Metastable pores at the onset of constant-current electroporation.

Kotulska M, Basalyga J, Derylo MB, Sadowski P - J. Membr. Biol. (2010)

Bottom Line: During this stage two species of metastable pores open, the majority of very low conductance that seem not fully developed as hydrophilic electropores.Membrane capacitance has a great impact on the membrane susceptibility to the pore appearance, related to its thickness and integrity.Pores of nonperfect membranes appear more easily, but they do not live any longer than others.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biomedical Engineering and Instrumentation, Wroclaw University of Technology, ul. Wybrzeze Wyspianskiego 27, 50-370, Wroclaw, Poland. malgorzata.kotulska@pwr.wroc.pl

ABSTRACT
Single metastable nanopores, appearing before the actual electroporation under constant-current conditions, are used to characterize the onset of electroporation. Unlike the long-lived electropores typical of the current controlled methods, these pores survive for milliseconds and observing them is possible due to slow development of electroporation, provided by the gradual accumulation of charges on a planar membrane. Analysis of the metastable pore appearance frequency and lifetime shows the first introductory stage of electroporation. During this stage two species of metastable pores open, the majority of very low conductance that seem not fully developed as hydrophilic electropores. The experiments reveal that voltage value defines the electroporation onset while the current value affects the rate of electroporation. Membrane capacitance has a great impact on the membrane susceptibility to the pore appearance, related to its thickness and integrity. Pores of nonperfect membranes appear more easily, but they do not live any longer than others.

Show MeSH
Metastable pores with low conductance are predominant and clearly separated from the higher-conductance pores, usually appearing close to full electroporation (histogram)
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2914259&req=5

Fig6: Metastable pores with low conductance are predominant and clearly separated from the higher-conductance pores, usually appearing close to full electroporation (histogram)

Mentions: The observed conductance of the metastable pores fell into the range 3.2–79.5 pS, although the low-conductance pores were predominant and vividly separated from the higher-conductance pores, usually appearing just before the actual electroporation, as presented in the histogram (Fig. 6). The majority of these high-conductance pores appeared at a current of 0.22 nA, generating the frequency maximum in Fig. 4.Fig. 6


Metastable pores at the onset of constant-current electroporation.

Kotulska M, Basalyga J, Derylo MB, Sadowski P - J. Membr. Biol. (2010)

Metastable pores with low conductance are predominant and clearly separated from the higher-conductance pores, usually appearing close to full electroporation (histogram)
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2914259&req=5

Fig6: Metastable pores with low conductance are predominant and clearly separated from the higher-conductance pores, usually appearing close to full electroporation (histogram)
Mentions: The observed conductance of the metastable pores fell into the range 3.2–79.5 pS, although the low-conductance pores were predominant and vividly separated from the higher-conductance pores, usually appearing just before the actual electroporation, as presented in the histogram (Fig. 6). The majority of these high-conductance pores appeared at a current of 0.22 nA, generating the frequency maximum in Fig. 4.Fig. 6

Bottom Line: During this stage two species of metastable pores open, the majority of very low conductance that seem not fully developed as hydrophilic electropores.Membrane capacitance has a great impact on the membrane susceptibility to the pore appearance, related to its thickness and integrity.Pores of nonperfect membranes appear more easily, but they do not live any longer than others.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biomedical Engineering and Instrumentation, Wroclaw University of Technology, ul. Wybrzeze Wyspianskiego 27, 50-370, Wroclaw, Poland. malgorzata.kotulska@pwr.wroc.pl

ABSTRACT
Single metastable nanopores, appearing before the actual electroporation under constant-current conditions, are used to characterize the onset of electroporation. Unlike the long-lived electropores typical of the current controlled methods, these pores survive for milliseconds and observing them is possible due to slow development of electroporation, provided by the gradual accumulation of charges on a planar membrane. Analysis of the metastable pore appearance frequency and lifetime shows the first introductory stage of electroporation. During this stage two species of metastable pores open, the majority of very low conductance that seem not fully developed as hydrophilic electropores. The experiments reveal that voltage value defines the electroporation onset while the current value affects the rate of electroporation. Membrane capacitance has a great impact on the membrane susceptibility to the pore appearance, related to its thickness and integrity. Pores of nonperfect membranes appear more easily, but they do not live any longer than others.

Show MeSH