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Calorie restriction and endurance exercise share potent anti-inflammatory function in adipose tissues in ameliorating diet-induced obesity and insulin resistance in mice.

Huang P, Li S, Shao M, Qi Q, Zhao F, You J, Mao T, Li W, Yan Z, Liu Y - Nutr Metab (Lond) (2010)

Bottom Line: The aim of this study was to directly compare the effects of CR and endurance exercise in a mouse model of diet-induced obesity and insulin resistance.CR dramatically prevented high-fat diet-induced metabolic abnormalities.CR and endurance exercise each potently suppressed the expression of inflammatory cytokines in white adipose tissues with additive effects when combined, but the effects of diet and exercise interventions in the liver were moderate to minimal.

View Article: PubMed Central - HTML - PubMed

Affiliation: Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences; Graduate School of the Chinese Academy of Sciences; Chinese Academy of Sciences, 294 Taiyuan Road, Shanghai 200031, China. zhen.yan@virginia.edu.

ABSTRACT

Background: Calorie restriction (CR) and endurance exercise are known to attenuate obesity and improve the metabolic syndrome. The aim of this study was to directly compare the effects of CR and endurance exercise in a mouse model of diet-induced obesity and insulin resistance.

Methods: Adult male C57BL/6N mice were randomly assigned and subjected to one of the six interventions for 8 weeks: low-fat diet (LC, 10% fat), low-fat diet with 30% calorie restriction (LR), high-fat diet (HC, 60% fat), high-fat diet with 30% calorie restriction (HR), high-fat diet with voluntary running exercise (HE), and high-fat diet with a combination of 30% calorie restriction and exercise (HRE). The impacts of the interventions were assessed by comprehensive metabolic analyses and pro-inflammatory cytokine gene expression.

Results: Endurance exercise significantly attenuated high-fat diet-induced obesity. CR dramatically prevented high-fat diet-induced metabolic abnormalities. A combination of CR and endurance exercise further reduced obesity and insulin resistance under the condition of high-fat diet. CR and endurance exercise each potently suppressed the expression of inflammatory cytokines in white adipose tissues with additive effects when combined, but the effects of diet and exercise interventions in the liver were moderate to minimal.

Conclusions: CR and endurance exercise share a potent anti-inflammatory function in adipose tissues in ameliorating diet-induced obesity and insulin resistance.

No MeSH data available.


Related in: MedlinePlus

Relationship between glucose intolerance and pro-inflammatory cytokine gene expression in adipose tissues versus the liver. Shown are correlations of the area under curve (AUC) for GTT with the mRNA expression level of OPN, TNF-a, MCP-1 and IL-6 in (A) white adipose tissues and (B) the liver. Data were derived as described in Methods. Spearman partial correlation coefficients for the AUC and each of the indicated pro-inflammatory cytokines were calculated after adjustment for interventions.
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Figure 7: Relationship between glucose intolerance and pro-inflammatory cytokine gene expression in adipose tissues versus the liver. Shown are correlations of the area under curve (AUC) for GTT with the mRNA expression level of OPN, TNF-a, MCP-1 and IL-6 in (A) white adipose tissues and (B) the liver. Data were derived as described in Methods. Spearman partial correlation coefficients for the AUC and each of the indicated pro-inflammatory cytokines were calculated after adjustment for interventions.

Mentions: To further investigate the possible cause of the impaired whole body glucose metabolism, we performed multivariate linear regression analysis on AUC of the glucose tolerance test and each of the metabolic and gene expression parameters (Table 1). As expected, calorie intake, body weight and fat mass showed significant correlation with AUC, indicating strong association of obesity with impaired whole body glucose metabolism. When compared between white adipose and liver tissues, AUC was highly correlated with the inflammatory cytokine gene expression in white adipose tissues (p < 0.0001 for all of the parameters), but was slightly correlated with these mRNAs in the liver for OPN (p < 0.05), TNF-α (p < 0.01) and IL-6 (p < 0.05) (Figure 7).


Calorie restriction and endurance exercise share potent anti-inflammatory function in adipose tissues in ameliorating diet-induced obesity and insulin resistance in mice.

Huang P, Li S, Shao M, Qi Q, Zhao F, You J, Mao T, Li W, Yan Z, Liu Y - Nutr Metab (Lond) (2010)

Relationship between glucose intolerance and pro-inflammatory cytokine gene expression in adipose tissues versus the liver. Shown are correlations of the area under curve (AUC) for GTT with the mRNA expression level of OPN, TNF-a, MCP-1 and IL-6 in (A) white adipose tissues and (B) the liver. Data were derived as described in Methods. Spearman partial correlation coefficients for the AUC and each of the indicated pro-inflammatory cytokines were calculated after adjustment for interventions.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2914080&req=5

Figure 7: Relationship between glucose intolerance and pro-inflammatory cytokine gene expression in adipose tissues versus the liver. Shown are correlations of the area under curve (AUC) for GTT with the mRNA expression level of OPN, TNF-a, MCP-1 and IL-6 in (A) white adipose tissues and (B) the liver. Data were derived as described in Methods. Spearman partial correlation coefficients for the AUC and each of the indicated pro-inflammatory cytokines were calculated after adjustment for interventions.
Mentions: To further investigate the possible cause of the impaired whole body glucose metabolism, we performed multivariate linear regression analysis on AUC of the glucose tolerance test and each of the metabolic and gene expression parameters (Table 1). As expected, calorie intake, body weight and fat mass showed significant correlation with AUC, indicating strong association of obesity with impaired whole body glucose metabolism. When compared between white adipose and liver tissues, AUC was highly correlated with the inflammatory cytokine gene expression in white adipose tissues (p < 0.0001 for all of the parameters), but was slightly correlated with these mRNAs in the liver for OPN (p < 0.05), TNF-α (p < 0.01) and IL-6 (p < 0.05) (Figure 7).

Bottom Line: The aim of this study was to directly compare the effects of CR and endurance exercise in a mouse model of diet-induced obesity and insulin resistance.CR dramatically prevented high-fat diet-induced metabolic abnormalities.CR and endurance exercise each potently suppressed the expression of inflammatory cytokines in white adipose tissues with additive effects when combined, but the effects of diet and exercise interventions in the liver were moderate to minimal.

View Article: PubMed Central - HTML - PubMed

Affiliation: Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences; Graduate School of the Chinese Academy of Sciences; Chinese Academy of Sciences, 294 Taiyuan Road, Shanghai 200031, China. zhen.yan@virginia.edu.

ABSTRACT

Background: Calorie restriction (CR) and endurance exercise are known to attenuate obesity and improve the metabolic syndrome. The aim of this study was to directly compare the effects of CR and endurance exercise in a mouse model of diet-induced obesity and insulin resistance.

Methods: Adult male C57BL/6N mice were randomly assigned and subjected to one of the six interventions for 8 weeks: low-fat diet (LC, 10% fat), low-fat diet with 30% calorie restriction (LR), high-fat diet (HC, 60% fat), high-fat diet with 30% calorie restriction (HR), high-fat diet with voluntary running exercise (HE), and high-fat diet with a combination of 30% calorie restriction and exercise (HRE). The impacts of the interventions were assessed by comprehensive metabolic analyses and pro-inflammatory cytokine gene expression.

Results: Endurance exercise significantly attenuated high-fat diet-induced obesity. CR dramatically prevented high-fat diet-induced metabolic abnormalities. A combination of CR and endurance exercise further reduced obesity and insulin resistance under the condition of high-fat diet. CR and endurance exercise each potently suppressed the expression of inflammatory cytokines in white adipose tissues with additive effects when combined, but the effects of diet and exercise interventions in the liver were moderate to minimal.

Conclusions: CR and endurance exercise share a potent anti-inflammatory function in adipose tissues in ameliorating diet-induced obesity and insulin resistance.

No MeSH data available.


Related in: MedlinePlus