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The timing of perinatal hypoxia/ischemia events in term neonates: a retrospective autopsy study. HSPs, ORP-150 and COX2 are reliable markers to classify acute, perinatal events.

Riezzo I, Neri M, De Stefano F, Fulcheri E, Ventura F, Pomara C, Rabozzi R, Turillazzi E, Fineschi V - Diagn Pathol (2010)

Bottom Line: COX2 reaction exhibited the strongest positive reaction in the neuronal cell bodies of acute cases, while a immunolabeling was prominent in the glial cytoplasm in the non-acute cases.Chaperones HSP70 and 90, ORP-150 reaction, and COX2 protein, have provided very interesting results.These results would suggest to the clinicians to extend the differential diagnosis of a too large perinatal hypoxic-ischemic insult category to delineate a more accurate chronological judgement.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Forensic Pathology, University of Foggia, Foggia, Italy.

ABSTRACT

Background: The understanding of the cellular responses implicated in perinatal brain damages and the characterization of the various mechanisms involved might open new horizons for understanding the time of onset of a brain hypoxic-ischemic lesion and for effective therapeutic strategies.

Methods: We performed an immunohistochemical investigation on brain and brainstem sections of 47 peripartum deaths. The gradation and localization of the expression of antibodies such as TNFalpha, IL-1beta, IL-6, HSPs, beta APP, anti-TrypH, GAP43, GFAP, COX2, ORP-150, could be correlated with an hypoxic-ischemic damage to document a significant correlation between response and the time of onset acute (/=8 hs

Results and discussions: In non-acute cases HSP70 reaction was prominent in the neuron cytoplasm, while in acute cases a mild reaction was evident in sporadic fields. HSP90 exhibited a similar pattern of positivity as HSP70. In acute group, ORP150 expressed an intense reaction showing a granular pattern in the cytoplasm of the neurons in the cortex of the infarcted areas. In non-acute group the positive reaction was more intense in astrocytes and less extended in neurons. COX2 reaction exhibited the strongest positive reaction in the neuronal cell bodies of acute cases, while a immunolabeling was prominent in the glial cytoplasm in the non-acute cases.

Conclusions: Chaperones HSP70 and 90, ORP-150 reaction, and COX2 protein, have provided very interesting results. These results would suggest to the clinicians to extend the differential diagnosis of a too large perinatal hypoxic-ischemic insult category to delineate a more accurate chronological judgement.

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(A) In non-acute cases (~10 hs) HSP70 reaction was prominent in the neuron cytoplasm of the affected regions. (B) (insert) Confocal laser scanning microscope: typical morphological features of neuronal (green) apoptosis associated with marked condensation of chromatin and its fragmentation into discrete bodies. (C-D) In acute cases a mild HSP70 reaction was evident in sporadic fields.
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Figure 3: (A) In non-acute cases (~10 hs) HSP70 reaction was prominent in the neuron cytoplasm of the affected regions. (B) (insert) Confocal laser scanning microscope: typical morphological features of neuronal (green) apoptosis associated with marked condensation of chromatin and its fragmentation into discrete bodies. (C-D) In acute cases a mild HSP70 reaction was evident in sporadic fields.

Mentions: Particularly significant was the immunohistochemical study of the brain and brainstem sections. In non-acute cases HSP70 reaction was prominent in the neuron cytoplasm of the affected regions, while in acute cases a mild reaction was evident in sporadic fields (Figure 3). HSP90 exhibited a similar pattern of positivity as HSP70 (Figure 4). In the acute group, ORP150 expressed an intense reaction showing a granular pattern in the cytoplasm of the neurons in the cortex of the infarcted areas. Differently, in the non-acute group the positive reaction was more intense in astrocytes and less extended in neurons (Figure 5). COX2 reaction exhibited a strong positive reaction in the neuronal cell bodies while immunolabeling was prominent in the glial cytoplasm in the non-acute cases (Figure 6).


The timing of perinatal hypoxia/ischemia events in term neonates: a retrospective autopsy study. HSPs, ORP-150 and COX2 are reliable markers to classify acute, perinatal events.

Riezzo I, Neri M, De Stefano F, Fulcheri E, Ventura F, Pomara C, Rabozzi R, Turillazzi E, Fineschi V - Diagn Pathol (2010)

(A) In non-acute cases (~10 hs) HSP70 reaction was prominent in the neuron cytoplasm of the affected regions. (B) (insert) Confocal laser scanning microscope: typical morphological features of neuronal (green) apoptosis associated with marked condensation of chromatin and its fragmentation into discrete bodies. (C-D) In acute cases a mild HSP70 reaction was evident in sporadic fields.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2914029&req=5

Figure 3: (A) In non-acute cases (~10 hs) HSP70 reaction was prominent in the neuron cytoplasm of the affected regions. (B) (insert) Confocal laser scanning microscope: typical morphological features of neuronal (green) apoptosis associated with marked condensation of chromatin and its fragmentation into discrete bodies. (C-D) In acute cases a mild HSP70 reaction was evident in sporadic fields.
Mentions: Particularly significant was the immunohistochemical study of the brain and brainstem sections. In non-acute cases HSP70 reaction was prominent in the neuron cytoplasm of the affected regions, while in acute cases a mild reaction was evident in sporadic fields (Figure 3). HSP90 exhibited a similar pattern of positivity as HSP70 (Figure 4). In the acute group, ORP150 expressed an intense reaction showing a granular pattern in the cytoplasm of the neurons in the cortex of the infarcted areas. Differently, in the non-acute group the positive reaction was more intense in astrocytes and less extended in neurons (Figure 5). COX2 reaction exhibited a strong positive reaction in the neuronal cell bodies while immunolabeling was prominent in the glial cytoplasm in the non-acute cases (Figure 6).

Bottom Line: COX2 reaction exhibited the strongest positive reaction in the neuronal cell bodies of acute cases, while a immunolabeling was prominent in the glial cytoplasm in the non-acute cases.Chaperones HSP70 and 90, ORP-150 reaction, and COX2 protein, have provided very interesting results.These results would suggest to the clinicians to extend the differential diagnosis of a too large perinatal hypoxic-ischemic insult category to delineate a more accurate chronological judgement.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Forensic Pathology, University of Foggia, Foggia, Italy.

ABSTRACT

Background: The understanding of the cellular responses implicated in perinatal brain damages and the characterization of the various mechanisms involved might open new horizons for understanding the time of onset of a brain hypoxic-ischemic lesion and for effective therapeutic strategies.

Methods: We performed an immunohistochemical investigation on brain and brainstem sections of 47 peripartum deaths. The gradation and localization of the expression of antibodies such as TNFalpha, IL-1beta, IL-6, HSPs, beta APP, anti-TrypH, GAP43, GFAP, COX2, ORP-150, could be correlated with an hypoxic-ischemic damage to document a significant correlation between response and the time of onset acute (/=8 hs

Results and discussions: In non-acute cases HSP70 reaction was prominent in the neuron cytoplasm, while in acute cases a mild reaction was evident in sporadic fields. HSP90 exhibited a similar pattern of positivity as HSP70. In acute group, ORP150 expressed an intense reaction showing a granular pattern in the cytoplasm of the neurons in the cortex of the infarcted areas. In non-acute group the positive reaction was more intense in astrocytes and less extended in neurons. COX2 reaction exhibited the strongest positive reaction in the neuronal cell bodies of acute cases, while a immunolabeling was prominent in the glial cytoplasm in the non-acute cases.

Conclusions: Chaperones HSP70 and 90, ORP-150 reaction, and COX2 protein, have provided very interesting results. These results would suggest to the clinicians to extend the differential diagnosis of a too large perinatal hypoxic-ischemic insult category to delineate a more accurate chronological judgement.

Show MeSH
Related in: MedlinePlus