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Radiation-induced DNA damage and repair in human gammadelta and alphabeta T-lymphocytes analysed by the alkaline comet assay.

Lisowska H, Deperas-Kaminska M, Haghdoost S, Parmryd I, Wojcik A - Genome Integr (2010)

Bottom Line: Gamma-delta T-lymphocytes play an important role in immunosurveillance and are promising cells for immunotherapy.The present investigation was carried out to test this.We found no difference in the level of DNA damage and the capacity of DNA repair between the T cell populations.

View Article: PubMed Central - HTML - PubMed

Affiliation: Jan Kochanowski University, Department of Radiobiology and Immunology, Kielce, Poland. andrzej.wojcik@gmt.su.se.

ABSTRACT
It has been shown by a number of authors that the radiosensitivity of peripheral blood mononuclear cells (PBMC) is higher in cancer patients compared to healthy donors, which is interpreted as a sign of genomic instability. PBMC are composed of different cell subpopulations which are differently radiosensitive and the difference between cancer patients and healthy donors could also be due to different composition of their PBMC pools. Gamma-delta T-lymphocytes play an important role in immunosurveillance and are promising cells for immunotherapy. Their abundance is frequently reduced in cancer patients so should their sensitivity to radiation be lower than that of other T-lymphocytes, this could, at least partly explain the low radiosensitivity of PBMC from healthy individuals compared to cancer patients. The present investigation was carried out to test this. Using the alkaline comet assay we analysed the level of DNA damage and repair in isolated gammadelta T-lymphocytes, pan T-lymphocytes and in total PBMC exposed in vitro to gamma radiation. We found no difference in the level of DNA damage and the capacity of DNA repair between the T cell populations. This is the first study that addresses the question of sensitivity to radiation of gamma-delta T-cells.

No MeSH data available.


Related in: MedlinePlus

Distributions of comets scored after 0 min repair (initial damage - panel A) and 60 min repair (residual damage - panel B). Cells were grouped into classes of percent of DNA in tail values. Pooled results from three experiments. Error bars: standard deviations from independent experiments. PT: pan T, GD: gamma-delta, PBMC: peripheral blood mononuclear cells.
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Figure 3: Distributions of comets scored after 0 min repair (initial damage - panel A) and 60 min repair (residual damage - panel B). Cells were grouped into classes of percent of DNA in tail values. Pooled results from three experiments. Error bars: standard deviations from independent experiments. PT: pan T, GD: gamma-delta, PBMC: peripheral blood mononuclear cells.

Mentions: An advantage of the comet assay is the ability to analyse DNA damage in individual cells. Although we observed no difference in the mean level of DNA damage between the analysed cell subpopulations, it is possible that differences existed in the distribution patterns of cells with different level of DNA damage. In order to check this, cells were grouped into classes of % TDNA values and plotted on one graph. The results for 0 and 60 min repair are shown in figure 3. The distribution patterns of all three cell populations look similar.


Radiation-induced DNA damage and repair in human gammadelta and alphabeta T-lymphocytes analysed by the alkaline comet assay.

Lisowska H, Deperas-Kaminska M, Haghdoost S, Parmryd I, Wojcik A - Genome Integr (2010)

Distributions of comets scored after 0 min repair (initial damage - panel A) and 60 min repair (residual damage - panel B). Cells were grouped into classes of percent of DNA in tail values. Pooled results from three experiments. Error bars: standard deviations from independent experiments. PT: pan T, GD: gamma-delta, PBMC: peripheral blood mononuclear cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2914012&req=5

Figure 3: Distributions of comets scored after 0 min repair (initial damage - panel A) and 60 min repair (residual damage - panel B). Cells were grouped into classes of percent of DNA in tail values. Pooled results from three experiments. Error bars: standard deviations from independent experiments. PT: pan T, GD: gamma-delta, PBMC: peripheral blood mononuclear cells.
Mentions: An advantage of the comet assay is the ability to analyse DNA damage in individual cells. Although we observed no difference in the mean level of DNA damage between the analysed cell subpopulations, it is possible that differences existed in the distribution patterns of cells with different level of DNA damage. In order to check this, cells were grouped into classes of % TDNA values and plotted on one graph. The results for 0 and 60 min repair are shown in figure 3. The distribution patterns of all three cell populations look similar.

Bottom Line: Gamma-delta T-lymphocytes play an important role in immunosurveillance and are promising cells for immunotherapy.The present investigation was carried out to test this.We found no difference in the level of DNA damage and the capacity of DNA repair between the T cell populations.

View Article: PubMed Central - HTML - PubMed

Affiliation: Jan Kochanowski University, Department of Radiobiology and Immunology, Kielce, Poland. andrzej.wojcik@gmt.su.se.

ABSTRACT
It has been shown by a number of authors that the radiosensitivity of peripheral blood mononuclear cells (PBMC) is higher in cancer patients compared to healthy donors, which is interpreted as a sign of genomic instability. PBMC are composed of different cell subpopulations which are differently radiosensitive and the difference between cancer patients and healthy donors could also be due to different composition of their PBMC pools. Gamma-delta T-lymphocytes play an important role in immunosurveillance and are promising cells for immunotherapy. Their abundance is frequently reduced in cancer patients so should their sensitivity to radiation be lower than that of other T-lymphocytes, this could, at least partly explain the low radiosensitivity of PBMC from healthy individuals compared to cancer patients. The present investigation was carried out to test this. Using the alkaline comet assay we analysed the level of DNA damage and repair in isolated gammadelta T-lymphocytes, pan T-lymphocytes and in total PBMC exposed in vitro to gamma radiation. We found no difference in the level of DNA damage and the capacity of DNA repair between the T cell populations. This is the first study that addresses the question of sensitivity to radiation of gamma-delta T-cells.

No MeSH data available.


Related in: MedlinePlus