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Radiation-induced DNA damage and repair in human gammadelta and alphabeta T-lymphocytes analysed by the alkaline comet assay.

Lisowska H, Deperas-Kaminska M, Haghdoost S, Parmryd I, Wojcik A - Genome Integr (2010)

Bottom Line: Gamma-delta T-lymphocytes play an important role in immunosurveillance and are promising cells for immunotherapy.The present investigation was carried out to test this.We found no difference in the level of DNA damage and the capacity of DNA repair between the T cell populations.

View Article: PubMed Central - HTML - PubMed

Affiliation: Jan Kochanowski University, Department of Radiobiology and Immunology, Kielce, Poland. andrzej.wojcik@gmt.su.se.

ABSTRACT
It has been shown by a number of authors that the radiosensitivity of peripheral blood mononuclear cells (PBMC) is higher in cancer patients compared to healthy donors, which is interpreted as a sign of genomic instability. PBMC are composed of different cell subpopulations which are differently radiosensitive and the difference between cancer patients and healthy donors could also be due to different composition of their PBMC pools. Gamma-delta T-lymphocytes play an important role in immunosurveillance and are promising cells for immunotherapy. Their abundance is frequently reduced in cancer patients so should their sensitivity to radiation be lower than that of other T-lymphocytes, this could, at least partly explain the low radiosensitivity of PBMC from healthy individuals compared to cancer patients. The present investigation was carried out to test this. Using the alkaline comet assay we analysed the level of DNA damage and repair in isolated gammadelta T-lymphocytes, pan T-lymphocytes and in total PBMC exposed in vitro to gamma radiation. We found no difference in the level of DNA damage and the capacity of DNA repair between the T cell populations. This is the first study that addresses the question of sensitivity to radiation of gamma-delta T-cells.

No MeSH data available.


Related in: MedlinePlus

Kinetics of DNA repair in cells from three independent experiments each with with lymphocytes of one donor donor (panels A-C) and pooled results (panel D). Net percent of DNA in tail values are shown. Error bars: standard deviations from individual cell measurements (panels A-C) and from the mean % TDNA values (panel D). PT: pan T, GD: gamma-delta, PBMC: peripheral blood mononuclear cells.
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Figure 2: Kinetics of DNA repair in cells from three independent experiments each with with lymphocytes of one donor donor (panels A-C) and pooled results (panel D). Net percent of DNA in tail values are shown. Error bars: standard deviations from individual cell measurements (panels A-C) and from the mean % TDNA values (panel D). PT: pan T, GD: gamma-delta, PBMC: peripheral blood mononuclear cells.

Mentions: The % TDNA values found in the control cells were subtracted from the radiation-induced values. The net radiation-induced % TDNA values as a function of repair time are shown in figure 2. No consistent difference between the cell subpopulations was observed for the initial level of DNA damage. DNA repair proceeded in all cell types at a similar rate and following 60 min of repair the majority of DNA damage was repaired. No consistent difference between the cell subpopulations was observed for the residual level damage.


Radiation-induced DNA damage and repair in human gammadelta and alphabeta T-lymphocytes analysed by the alkaline comet assay.

Lisowska H, Deperas-Kaminska M, Haghdoost S, Parmryd I, Wojcik A - Genome Integr (2010)

Kinetics of DNA repair in cells from three independent experiments each with with lymphocytes of one donor donor (panels A-C) and pooled results (panel D). Net percent of DNA in tail values are shown. Error bars: standard deviations from individual cell measurements (panels A-C) and from the mean % TDNA values (panel D). PT: pan T, GD: gamma-delta, PBMC: peripheral blood mononuclear cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2914012&req=5

Figure 2: Kinetics of DNA repair in cells from three independent experiments each with with lymphocytes of one donor donor (panels A-C) and pooled results (panel D). Net percent of DNA in tail values are shown. Error bars: standard deviations from individual cell measurements (panels A-C) and from the mean % TDNA values (panel D). PT: pan T, GD: gamma-delta, PBMC: peripheral blood mononuclear cells.
Mentions: The % TDNA values found in the control cells were subtracted from the radiation-induced values. The net radiation-induced % TDNA values as a function of repair time are shown in figure 2. No consistent difference between the cell subpopulations was observed for the initial level of DNA damage. DNA repair proceeded in all cell types at a similar rate and following 60 min of repair the majority of DNA damage was repaired. No consistent difference between the cell subpopulations was observed for the residual level damage.

Bottom Line: Gamma-delta T-lymphocytes play an important role in immunosurveillance and are promising cells for immunotherapy.The present investigation was carried out to test this.We found no difference in the level of DNA damage and the capacity of DNA repair between the T cell populations.

View Article: PubMed Central - HTML - PubMed

Affiliation: Jan Kochanowski University, Department of Radiobiology and Immunology, Kielce, Poland. andrzej.wojcik@gmt.su.se.

ABSTRACT
It has been shown by a number of authors that the radiosensitivity of peripheral blood mononuclear cells (PBMC) is higher in cancer patients compared to healthy donors, which is interpreted as a sign of genomic instability. PBMC are composed of different cell subpopulations which are differently radiosensitive and the difference between cancer patients and healthy donors could also be due to different composition of their PBMC pools. Gamma-delta T-lymphocytes play an important role in immunosurveillance and are promising cells for immunotherapy. Their abundance is frequently reduced in cancer patients so should their sensitivity to radiation be lower than that of other T-lymphocytes, this could, at least partly explain the low radiosensitivity of PBMC from healthy individuals compared to cancer patients. The present investigation was carried out to test this. Using the alkaline comet assay we analysed the level of DNA damage and repair in isolated gammadelta T-lymphocytes, pan T-lymphocytes and in total PBMC exposed in vitro to gamma radiation. We found no difference in the level of DNA damage and the capacity of DNA repair between the T cell populations. This is the first study that addresses the question of sensitivity to radiation of gamma-delta T-cells.

No MeSH data available.


Related in: MedlinePlus