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Tocopherol induced angiogenesis in placental vascular network in late pregnant ewes.

Kasimanickam RK, Kasimanickam VR, Rodriguez JS, Pelzer KD, Sponenberg PD, Thatcher CD - Reprod. Biol. Endocrinol. (2010)

Bottom Line: Tocopherols have biphasic, proangiogenic and antiangiogenic therapeutic effects.In aT group, mRNA expressions of PlGF, eNOS and HIF-1alpha in cotyledon were greater than the CON group.Increase in placental angiogenesis may provide nutrients required for the development and growth of fetus during late pregnancy.

View Article: PubMed Central - HTML - PubMed

Affiliation: College of Veterinary Medicine, Washington State University, Pullman, WA 99164, USA. ramkasi@vetmed.wsu.edu

ABSTRACT

Background: Tocopherols have biphasic, proangiogenic and antiangiogenic therapeutic effects. The objective of this clinical trial was to clarify tocopherol's placental angiogenic potential in late pregnant ewes following oral supplementation.

Methods: Eighteen pregnant ewes during late gestation were selected for this study. Ewes were given oral supplementation of 500 mg of alpha-tocopherol (aT; N=6) or 1000 mg of gamma-tocopherol (gT; N=7) or placebo (CON; N=5) once daily from 107 to 137 days post breeding. Serum was obtained at weekly intervals and tissue samples were obtained at the end of supplementation to: 1) evaluate tocopherol concentrations in serum, uterus and placentome; 2) evaluate relative mRNA expressions of Vascular Endothelial Growth Factor (VEGF), Placental Growth Factor (PlGF), endothelial Nitric Oxide Synthase (eNOS) and Hypoxia Inducible Factors (HIF) in uterus, caruncle and cotyledon; 3) analyze the morphometry of the placental vascular network.

Results: Supplementation of aT or gT resulted in increased concentrations in serum, placentome and uterus compared to control (P<0.05). In aT group, mRNA expressions of PlGF, eNOS and HIF-1alpha in cotyledon were greater than the CON group. In gT group, mRNA expressions of VEGF, eNOS, HIF-1 alpha and HIF-2 alpha in caruncle and uterus, and HIF-1alpha in cotyledon, were greater than the CON group. Morphometry analysis revealed increased angiogenesis in the supplemented groups.

Conclusion: Daily oral supplementation of aT or gT increased angiogenesis in the placental vascular network in pregnant ewes during late gestation. Increase in placental angiogenesis may provide nutrients required for the development and growth of fetus during late pregnancy.

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Fractal dimension and lacunarity of placental vascular network of late pregnant ewes supplemented with alpha tocopherol, gamma tocopherol or placebo. Bar graph represents expression of fractal dimension and lacunarity of treated groups. Groups with different letters were significant (P < 0.05).
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Figure 6: Fractal dimension and lacunarity of placental vascular network of late pregnant ewes supplemented with alpha tocopherol, gamma tocopherol or placebo. Bar graph represents expression of fractal dimension and lacunarity of treated groups. Groups with different letters were significant (P < 0.05).

Mentions: The results showed that aT or gT supplemented ewes had increased fractal dimension and decreased lacunarity in their placental vascular network compared to CON ewes (Figure 6; P < 0.05).


Tocopherol induced angiogenesis in placental vascular network in late pregnant ewes.

Kasimanickam RK, Kasimanickam VR, Rodriguez JS, Pelzer KD, Sponenberg PD, Thatcher CD - Reprod. Biol. Endocrinol. (2010)

Fractal dimension and lacunarity of placental vascular network of late pregnant ewes supplemented with alpha tocopherol, gamma tocopherol or placebo. Bar graph represents expression of fractal dimension and lacunarity of treated groups. Groups with different letters were significant (P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2913989&req=5

Figure 6: Fractal dimension and lacunarity of placental vascular network of late pregnant ewes supplemented with alpha tocopherol, gamma tocopherol or placebo. Bar graph represents expression of fractal dimension and lacunarity of treated groups. Groups with different letters were significant (P < 0.05).
Mentions: The results showed that aT or gT supplemented ewes had increased fractal dimension and decreased lacunarity in their placental vascular network compared to CON ewes (Figure 6; P < 0.05).

Bottom Line: Tocopherols have biphasic, proangiogenic and antiangiogenic therapeutic effects.In aT group, mRNA expressions of PlGF, eNOS and HIF-1alpha in cotyledon were greater than the CON group.Increase in placental angiogenesis may provide nutrients required for the development and growth of fetus during late pregnancy.

View Article: PubMed Central - HTML - PubMed

Affiliation: College of Veterinary Medicine, Washington State University, Pullman, WA 99164, USA. ramkasi@vetmed.wsu.edu

ABSTRACT

Background: Tocopherols have biphasic, proangiogenic and antiangiogenic therapeutic effects. The objective of this clinical trial was to clarify tocopherol's placental angiogenic potential in late pregnant ewes following oral supplementation.

Methods: Eighteen pregnant ewes during late gestation were selected for this study. Ewes were given oral supplementation of 500 mg of alpha-tocopherol (aT; N=6) or 1000 mg of gamma-tocopherol (gT; N=7) or placebo (CON; N=5) once daily from 107 to 137 days post breeding. Serum was obtained at weekly intervals and tissue samples were obtained at the end of supplementation to: 1) evaluate tocopherol concentrations in serum, uterus and placentome; 2) evaluate relative mRNA expressions of Vascular Endothelial Growth Factor (VEGF), Placental Growth Factor (PlGF), endothelial Nitric Oxide Synthase (eNOS) and Hypoxia Inducible Factors (HIF) in uterus, caruncle and cotyledon; 3) analyze the morphometry of the placental vascular network.

Results: Supplementation of aT or gT resulted in increased concentrations in serum, placentome and uterus compared to control (P<0.05). In aT group, mRNA expressions of PlGF, eNOS and HIF-1alpha in cotyledon were greater than the CON group. In gT group, mRNA expressions of VEGF, eNOS, HIF-1 alpha and HIF-2 alpha in caruncle and uterus, and HIF-1alpha in cotyledon, were greater than the CON group. Morphometry analysis revealed increased angiogenesis in the supplemented groups.

Conclusion: Daily oral supplementation of aT or gT increased angiogenesis in the placental vascular network in pregnant ewes during late gestation. Increase in placental angiogenesis may provide nutrients required for the development and growth of fetus during late pregnancy.

Show MeSH
Related in: MedlinePlus