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Hepatitis B virus genotypes and precore and core mutants in UAE patients.

Alfaresi M, Elkoush A, Alshehhi H, Alzaabi A, Islam A - Virol. J. (2010)

Bottom Line: Precore mutants were found in 51 (58.0%) of 88 patients.Mutations in the basal core promotor were found in 22 (25.3%) of 88 patients.Mutations in the basal core promoter were found in 22 of 88 (25.3%) samples.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pathology &Laboratory Medicine, Zayed Military Hospital, Abu Dhabi, UAE. uaenow@eim.ae

ABSTRACT

Background: Knowledge of the HBV genotype with which a patient is infected is crucial information for a physician to have when planning clinical treatment for that patient. Previous studies have suggested that there are possible differences in the pathogenicity and therapeutic response of different HBV genotypes. However, the prevalence of the various HBV genotypes and Precore and Core mutations is unknown in the UAE. Therefore, we sought to determine the prevalence of the different HBV genotypes in the UAE population.

Methodology/principal findings: A total of 88 HBsAg-positive patients were included in the study. A method for genotyping and subtyping HBV by partial HBsAg gene sequencing using primers that are complementary to all known genotypes was used. Precore and core region of these viruses were also sequenced in 88 patients.HBV genotype D was the most prevalent (79.5%) genotype identified in our study population, followed by genotypes A (18.2%) and C (2.3%). The following subtypes were isolated: ayw2 (80.7%), adw2 (14.8%), and adw (2.3%). The HBV-DNA viral load was higher in HBeAg-positive patients than it was in patients who were HBeAg-negative. Precore mutants were found in 51 (58.0%) of 88 patients. Mutations in the basal core promotor were found in 22 (25.3%) of 88 patients.

Conclusion/significance: HBV infection is a major health problem in the UAE, and while genotypes B and C are the most prevalent HBV genotypes in the Asian population, our study reveals that genotype D is the predominant genotype that is present in the UAE. More patients were HBeAg-negative than were HBeAg-positive in our study sample, which could be due to the duration of infection of the included patients. Additionally, the viral loads of the HBeAg-positive patients were higher those of the HBeAg-negative patients. Analysis of nucleotide 1858 showed presence of thymine in all patients with genotypes C, and D and in a few patients with genotypes A. This nucleotide was closely related to the presence of precore mutants. Mutations in the basal core promoter were found in 22 of 88 (25.3%) samples. These mutations were more frequent in patients infected with genotype A (37.5%) and not found in patients infected with genotype C.

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Neighbor-joining tree of a specific HBV-DNA nucleotide fragment (S gene) from viral isolates obtained from the study population. Woolly monkey Hepatitis B virus (GenBank accession number AF046996) was used as an outgroup.
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Figure 1: Neighbor-joining tree of a specific HBV-DNA nucleotide fragment (S gene) from viral isolates obtained from the study population. Woolly monkey Hepatitis B virus (GenBank accession number AF046996) was used as an outgroup.

Mentions: The baseline characteristics of the study population as well as the frequencies with which the various HBV genotypes and subtypes were observed are shown in Table 1. All 88 patients (100%) were citizens of the UAE. In this group [as was shown in phylogenetic analysis (Fig. 1)], genotype D was the most prevalent (79.5%) followed by genotype A (18.2%) and genotype C (2.3%). The following subtypes were detected: ayw2 (80.7%), adw2 (14.8%), and adw (2.3%). Two patients infected with HBV genotype C could not be subtyped. Within genotype A, subtypes adw and adw2 were detected, while within genotype D, only subtype ayw2 was detected.


Hepatitis B virus genotypes and precore and core mutants in UAE patients.

Alfaresi M, Elkoush A, Alshehhi H, Alzaabi A, Islam A - Virol. J. (2010)

Neighbor-joining tree of a specific HBV-DNA nucleotide fragment (S gene) from viral isolates obtained from the study population. Woolly monkey Hepatitis B virus (GenBank accession number AF046996) was used as an outgroup.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2913959&req=5

Figure 1: Neighbor-joining tree of a specific HBV-DNA nucleotide fragment (S gene) from viral isolates obtained from the study population. Woolly monkey Hepatitis B virus (GenBank accession number AF046996) was used as an outgroup.
Mentions: The baseline characteristics of the study population as well as the frequencies with which the various HBV genotypes and subtypes were observed are shown in Table 1. All 88 patients (100%) were citizens of the UAE. In this group [as was shown in phylogenetic analysis (Fig. 1)], genotype D was the most prevalent (79.5%) followed by genotype A (18.2%) and genotype C (2.3%). The following subtypes were detected: ayw2 (80.7%), adw2 (14.8%), and adw (2.3%). Two patients infected with HBV genotype C could not be subtyped. Within genotype A, subtypes adw and adw2 were detected, while within genotype D, only subtype ayw2 was detected.

Bottom Line: Precore mutants were found in 51 (58.0%) of 88 patients.Mutations in the basal core promotor were found in 22 (25.3%) of 88 patients.Mutations in the basal core promoter were found in 22 of 88 (25.3%) samples.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pathology &Laboratory Medicine, Zayed Military Hospital, Abu Dhabi, UAE. uaenow@eim.ae

ABSTRACT

Background: Knowledge of the HBV genotype with which a patient is infected is crucial information for a physician to have when planning clinical treatment for that patient. Previous studies have suggested that there are possible differences in the pathogenicity and therapeutic response of different HBV genotypes. However, the prevalence of the various HBV genotypes and Precore and Core mutations is unknown in the UAE. Therefore, we sought to determine the prevalence of the different HBV genotypes in the UAE population.

Methodology/principal findings: A total of 88 HBsAg-positive patients were included in the study. A method for genotyping and subtyping HBV by partial HBsAg gene sequencing using primers that are complementary to all known genotypes was used. Precore and core region of these viruses were also sequenced in 88 patients.HBV genotype D was the most prevalent (79.5%) genotype identified in our study population, followed by genotypes A (18.2%) and C (2.3%). The following subtypes were isolated: ayw2 (80.7%), adw2 (14.8%), and adw (2.3%). The HBV-DNA viral load was higher in HBeAg-positive patients than it was in patients who were HBeAg-negative. Precore mutants were found in 51 (58.0%) of 88 patients. Mutations in the basal core promotor were found in 22 (25.3%) of 88 patients.

Conclusion/significance: HBV infection is a major health problem in the UAE, and while genotypes B and C are the most prevalent HBV genotypes in the Asian population, our study reveals that genotype D is the predominant genotype that is present in the UAE. More patients were HBeAg-negative than were HBeAg-positive in our study sample, which could be due to the duration of infection of the included patients. Additionally, the viral loads of the HBeAg-positive patients were higher those of the HBeAg-negative patients. Analysis of nucleotide 1858 showed presence of thymine in all patients with genotypes C, and D and in a few patients with genotypes A. This nucleotide was closely related to the presence of precore mutants. Mutations in the basal core promoter were found in 22 of 88 (25.3%) samples. These mutations were more frequent in patients infected with genotype A (37.5%) and not found in patients infected with genotype C.

Show MeSH
Related in: MedlinePlus