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Overexpression of candidate tumor suppressor ECRG4 inhibits glioma proliferation and invasion.

Li W, Liu X, Zhang B, Qi D, Zhang L, Jin Y, Yang H - J. Exp. Clin. Cancer Res. (2010)

Bottom Line: Cells transfected with ECRG4 showed significantly decreased cell proliferation as evaluated by MTT and colony formation assays.Furthermore, overexpression inhibited cell migration and invasion in transwell and Boyden chamber experiments and retarded the cell cycle progression from G1 to S phase by FACSCaliber cytometry.Protein levels of nuclear transcription factor NF-kB, which is involved in cell proliferation, inversely correlated with ECRG4 expression.

View Article: PubMed Central - HTML - PubMed

Affiliation: The Key Laboratory of Pathobiology, Ministry of Education, Jilin University, 130021, PR China.

ABSTRACT

Background: ECRG4 has been shown to be a candidate tumor suppressor in several tumors, but its role in glioma remains poorly understood. In this study, we examined the mRNA expression of ECRG4 and investigated its biological role in glioma cells.

Methods: Real-time PCR was used to examine expression of ECRG4 in gliomas and their matched brain tissues. The effect of ECRG4 expression on cell proliferation, invasion, and migration was investigated in human U251 glioma cells. Finally, the regulation of transcription factor NF-kB by ECRG4 was evaluated by western blotting.

Results: Of the 10 paired samples analyzed, 9 glioma tissues displayed the decreased expression of ECRG4 compared to matched normal brain tissues. Cells transfected with ECRG4 showed significantly decreased cell proliferation as evaluated by MTT and colony formation assays. Furthermore, overexpression inhibited cell migration and invasion in transwell and Boyden chamber experiments and retarded the cell cycle progression from G1 to S phase by FACSCaliber cytometry. Protein levels of nuclear transcription factor NF-kB, which is involved in cell proliferation, inversely correlated with ECRG4 expression.

Conclusion: Our data suggest that ECRG4 serves as a tumor suppressor in glioma.

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Related in: MedlinePlus

The reduced expression levels of ECRG4 mRNA in glioma. A. ECRG4 mRNA level was markedly downregualted in glioma tissue comparing to their matched normal brain tissues. (T: Tumor; N: Normal tissue).
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Figure 1: The reduced expression levels of ECRG4 mRNA in glioma. A. ECRG4 mRNA level was markedly downregualted in glioma tissue comparing to their matched normal brain tissues. (T: Tumor; N: Normal tissue).

Mentions: In order to assess the role of ECRG4 in glioma, we performed real-time PCR to measure the expression of ECRG4 mRNA transcripts in 10 paired gliomas and their adjacent brain tissues. As shown in Figure 1A, 9 glioma tissues showed markedly decreased expression (>2-fold change) of ECRG4 compared to their matched normal tissues.


Overexpression of candidate tumor suppressor ECRG4 inhibits glioma proliferation and invasion.

Li W, Liu X, Zhang B, Qi D, Zhang L, Jin Y, Yang H - J. Exp. Clin. Cancer Res. (2010)

The reduced expression levels of ECRG4 mRNA in glioma. A. ECRG4 mRNA level was markedly downregualted in glioma tissue comparing to their matched normal brain tissues. (T: Tumor; N: Normal tissue).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2913949&req=5

Figure 1: The reduced expression levels of ECRG4 mRNA in glioma. A. ECRG4 mRNA level was markedly downregualted in glioma tissue comparing to their matched normal brain tissues. (T: Tumor; N: Normal tissue).
Mentions: In order to assess the role of ECRG4 in glioma, we performed real-time PCR to measure the expression of ECRG4 mRNA transcripts in 10 paired gliomas and their adjacent brain tissues. As shown in Figure 1A, 9 glioma tissues showed markedly decreased expression (>2-fold change) of ECRG4 compared to their matched normal tissues.

Bottom Line: Cells transfected with ECRG4 showed significantly decreased cell proliferation as evaluated by MTT and colony formation assays.Furthermore, overexpression inhibited cell migration and invasion in transwell and Boyden chamber experiments and retarded the cell cycle progression from G1 to S phase by FACSCaliber cytometry.Protein levels of nuclear transcription factor NF-kB, which is involved in cell proliferation, inversely correlated with ECRG4 expression.

View Article: PubMed Central - HTML - PubMed

Affiliation: The Key Laboratory of Pathobiology, Ministry of Education, Jilin University, 130021, PR China.

ABSTRACT

Background: ECRG4 has been shown to be a candidate tumor suppressor in several tumors, but its role in glioma remains poorly understood. In this study, we examined the mRNA expression of ECRG4 and investigated its biological role in glioma cells.

Methods: Real-time PCR was used to examine expression of ECRG4 in gliomas and their matched brain tissues. The effect of ECRG4 expression on cell proliferation, invasion, and migration was investigated in human U251 glioma cells. Finally, the regulation of transcription factor NF-kB by ECRG4 was evaluated by western blotting.

Results: Of the 10 paired samples analyzed, 9 glioma tissues displayed the decreased expression of ECRG4 compared to matched normal brain tissues. Cells transfected with ECRG4 showed significantly decreased cell proliferation as evaluated by MTT and colony formation assays. Furthermore, overexpression inhibited cell migration and invasion in transwell and Boyden chamber experiments and retarded the cell cycle progression from G1 to S phase by FACSCaliber cytometry. Protein levels of nuclear transcription factor NF-kB, which is involved in cell proliferation, inversely correlated with ECRG4 expression.

Conclusion: Our data suggest that ECRG4 serves as a tumor suppressor in glioma.

Show MeSH
Related in: MedlinePlus