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Malignant mixed Mullerian tumors of the uterus: histopathological evaluation of cell cycle and apoptotic regulatory proteins.

Kanthan R, Senger JL, Diudea D - World J Surg Oncol (2010)

Bottom Line: Overexpression of p16 and Mcl-1 were observed in patients with longer survival outcomes (>2 years).P16 and p21 were overexpressed in the carcinomatous and sarcomatous elements respectively.Cyclin-D1 was focally expressed only in the carcinomatous elements.

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Affiliation: Department of Pathology and Laboratory Medicine, University of Saskatchewan, Saskatoon, SK, Canada. rani.kanthan@saskatoonhealthregion.ca

ABSTRACT

Aim: The aim of our study was to evaluate survival outcomes in malignant mixed Mullerian tumors (MMMT) of the uterus with respect to the role of cell cycle and apoptotic regulatory proteins in the carcinomatous and sarcomatous components.

Methods: 23 cases of uterine MMMT identified from the Saskatchewan Cancer Agency (1970-1999) were evaluated. Immunohistochemical expression of Bad, Mcl-1, bcl-x, bak, mdm2, bax, p16, p21, p53, p27, EMA, Bcl-2, Ki67 and PCNA was correlated with clinico-pathological data including survival outcomes.

Results: Histopathological examination confirmed malignant epithelial component with homologous (12 cases) and heterologous (11 cases) sarcomatous elements. P53 was strongly expressed (70-95%) in 15 cases and negative in 5 cases. The average survival in the p53+ve cases was 3.56 years as opposed to 8.94 years in p53-ve cases. Overexpression of p16 and Mcl-1 were observed in patients with longer survival outcomes (>2 years). P16 and p21 were overexpressed in the carcinomatous and sarcomatous elements respectively. Cyclin-D1 was focally expressed only in the carcinomatous elements.

Conclusions: Our study supports that a) cell cycle and apoptotic regulatory protein dysregulation is an important pathway for tumorigenesis and b) p53 is an important immunoprognostic marker in MMMT of the uterus.

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Cell Cycle and Apoptotic Regulatory Proteins in Relation to 2 Year Survival Data. Statistical significance: *p < 0.05, †p < 0.10.
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Figure 3: Cell Cycle and Apoptotic Regulatory Proteins in Relation to 2 Year Survival Data. Statistical significance: *p < 0.05, †p < 0.10.

Mentions: Cell cycle and apoptotic regulatory proteins were analyzed for statistical significance as possible prognostic indicators. Two cell cycle proteins, Mcl-1 and p16, were found to be statistically significant. Of the 23 patients, 8 (34.8%) were positive for Mcl-1. As seen in Figure 3, survivors of 2 years, 53.8% had a positive Mcl-1 expression, while only 10.0% of those cases that did not survive showed positive Mcl-1 expression. Similar results were obtained for p16. Seven cases (30.4%) were positive for this cell cycle protein. Of the patients who survived two years, 46.2% exhibited positive p16 expression, while only 10.0% of those who did not survive 2 years did. P53 was strongly expressed (70-95%) in 15 cases and negative in 5 cases. The average survival in the P53+ve cases was 3.56 years as opposed to 8.94 years in P53-ve cases.


Malignant mixed Mullerian tumors of the uterus: histopathological evaluation of cell cycle and apoptotic regulatory proteins.

Kanthan R, Senger JL, Diudea D - World J Surg Oncol (2010)

Cell Cycle and Apoptotic Regulatory Proteins in Relation to 2 Year Survival Data. Statistical significance: *p < 0.05, †p < 0.10.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2913917&req=5

Figure 3: Cell Cycle and Apoptotic Regulatory Proteins in Relation to 2 Year Survival Data. Statistical significance: *p < 0.05, †p < 0.10.
Mentions: Cell cycle and apoptotic regulatory proteins were analyzed for statistical significance as possible prognostic indicators. Two cell cycle proteins, Mcl-1 and p16, were found to be statistically significant. Of the 23 patients, 8 (34.8%) were positive for Mcl-1. As seen in Figure 3, survivors of 2 years, 53.8% had a positive Mcl-1 expression, while only 10.0% of those cases that did not survive showed positive Mcl-1 expression. Similar results were obtained for p16. Seven cases (30.4%) were positive for this cell cycle protein. Of the patients who survived two years, 46.2% exhibited positive p16 expression, while only 10.0% of those who did not survive 2 years did. P53 was strongly expressed (70-95%) in 15 cases and negative in 5 cases. The average survival in the P53+ve cases was 3.56 years as opposed to 8.94 years in P53-ve cases.

Bottom Line: Overexpression of p16 and Mcl-1 were observed in patients with longer survival outcomes (>2 years).P16 and p21 were overexpressed in the carcinomatous and sarcomatous elements respectively.Cyclin-D1 was focally expressed only in the carcinomatous elements.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pathology and Laboratory Medicine, University of Saskatchewan, Saskatoon, SK, Canada. rani.kanthan@saskatoonhealthregion.ca

ABSTRACT

Aim: The aim of our study was to evaluate survival outcomes in malignant mixed Mullerian tumors (MMMT) of the uterus with respect to the role of cell cycle and apoptotic regulatory proteins in the carcinomatous and sarcomatous components.

Methods: 23 cases of uterine MMMT identified from the Saskatchewan Cancer Agency (1970-1999) were evaluated. Immunohistochemical expression of Bad, Mcl-1, bcl-x, bak, mdm2, bax, p16, p21, p53, p27, EMA, Bcl-2, Ki67 and PCNA was correlated with clinico-pathological data including survival outcomes.

Results: Histopathological examination confirmed malignant epithelial component with homologous (12 cases) and heterologous (11 cases) sarcomatous elements. P53 was strongly expressed (70-95%) in 15 cases and negative in 5 cases. The average survival in the p53+ve cases was 3.56 years as opposed to 8.94 years in p53-ve cases. Overexpression of p16 and Mcl-1 were observed in patients with longer survival outcomes (>2 years). P16 and p21 were overexpressed in the carcinomatous and sarcomatous elements respectively. Cyclin-D1 was focally expressed only in the carcinomatous elements.

Conclusions: Our study supports that a) cell cycle and apoptotic regulatory protein dysregulation is an important pathway for tumorigenesis and b) p53 is an important immunoprognostic marker in MMMT of the uterus.

Show MeSH
Related in: MedlinePlus