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Regulatory effect of melatonin on cytokine disturbances in the pristane-induced lupus mice.

Zhou LL, Wei W, Si JF, Yuan DP - Mediators Inflamm. (2010)

Bottom Line: The results showed that melatonin antagonized the increasing levels of IgM anti ssDNA and histone autoantibodies.Melatonin could also decrease the IL-6 and IL-13 production and increase the IL-2 production.These results suggested that melatonin has a beneficial effect on pristane-induced lupus through regulating the cytokines disturbances.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Anti-inflammatory and immunopharmacology in Education Ministry of China, Institute of Clinical Pharmacology, Anhui Medical University, Hefei 230032, China.

ABSTRACT
Systemic lupus erythematosus (SLE) develops in relation to many environmental factors. In our opinion, it is more important to investigate the effect of melatonin on the environmental- related SLE. In the present study, 0.5 ml pristane were used to induce SLE in female BALB/c mice. Melatonin (0.01, 0.1, 1.0 mg/kg) was orally administered immediately after pristane-injection for 24 weeks. IgM anti ssDNA and histone antibodies were detected after 0, 1, 2, 4, 8 weeks pristane injection. The levels of IL-2, IL-6 and IL-13 were detected after 24 weeks. Renal lesions were also observed. The results showed that melatonin antagonized the increasing levels of IgM anti ssDNA and histone autoantibodies. Melatonin could also decrease the IL-6 and IL-13 production and increase the IL-2 production. Besides, melatonin could lessen the renal lesions caused by pristane. These results suggested that melatonin has a beneficial effect on pristane-induced lupus through regulating the cytokines disturbances.

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Related in: MedlinePlus

All mice were sacrificed at the end of 24 weeks, and splenic lymphocytes were seeded at 1 × 106 cells/well. IL-2 concentrations in splenic lymphocytes were stimulated for 48 h with 3 mg/L ConA. IL-6, and IL-13 concentrations in splenic lymphocytes were stimulated for 48 h with 12 mg/L LPS. IL-2, IL-6, and IL-13 concentrations in culture supernatants were detected by ELISA. Data were given in mean± SD (n = 4–5). **P < .01, *P < .05 versus Normal control group,  ∆P < .05, ∆∆P < .01 versus Model control group.
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fig2: All mice were sacrificed at the end of 24 weeks, and splenic lymphocytes were seeded at 1 × 106 cells/well. IL-2 concentrations in splenic lymphocytes were stimulated for 48 h with 3 mg/L ConA. IL-6, and IL-13 concentrations in splenic lymphocytes were stimulated for 48 h with 12 mg/L LPS. IL-2, IL-6, and IL-13 concentrations in culture supernatants were detected by ELISA. Data were given in mean± SD (n = 4–5). **P < .01, *P < .05 versus Normal control group, ∆P < .05, ∆∆P < .01 versus Model control group.

Mentions: To gain a better insight into the influence of melatonin on cytokines in SLE, production of Th1-type and Th2-type cytokines by splenocytes stimulated with ConA or LPS was assayed during the course of the murine lupus. The results showed that the production of IL-2, IL-6, and IL-13 changed in pristane-induced SLE mice (Figure 2). IL-2 production of splenocytes from mice in model control group was lower than that from normal mice (P < .05), while IL-6 and IL-13 production of splenocytes from mice in model control group was higher than that from normal mice (P < .01). In MT2 and MT3 groups, IL-2 levels were up to the normal level and higher than that of model mice obviously (P < .05), while the IL-6 and IL-13 levels were lower than that of the model mice obviously (P < .05, .01) (Figure 2).


Regulatory effect of melatonin on cytokine disturbances in the pristane-induced lupus mice.

Zhou LL, Wei W, Si JF, Yuan DP - Mediators Inflamm. (2010)

All mice were sacrificed at the end of 24 weeks, and splenic lymphocytes were seeded at 1 × 106 cells/well. IL-2 concentrations in splenic lymphocytes were stimulated for 48 h with 3 mg/L ConA. IL-6, and IL-13 concentrations in splenic lymphocytes were stimulated for 48 h with 12 mg/L LPS. IL-2, IL-6, and IL-13 concentrations in culture supernatants were detected by ELISA. Data were given in mean± SD (n = 4–5). **P < .01, *P < .05 versus Normal control group,  ∆P < .05, ∆∆P < .01 versus Model control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2913856&req=5

fig2: All mice were sacrificed at the end of 24 weeks, and splenic lymphocytes were seeded at 1 × 106 cells/well. IL-2 concentrations in splenic lymphocytes were stimulated for 48 h with 3 mg/L ConA. IL-6, and IL-13 concentrations in splenic lymphocytes were stimulated for 48 h with 12 mg/L LPS. IL-2, IL-6, and IL-13 concentrations in culture supernatants were detected by ELISA. Data were given in mean± SD (n = 4–5). **P < .01, *P < .05 versus Normal control group, ∆P < .05, ∆∆P < .01 versus Model control group.
Mentions: To gain a better insight into the influence of melatonin on cytokines in SLE, production of Th1-type and Th2-type cytokines by splenocytes stimulated with ConA or LPS was assayed during the course of the murine lupus. The results showed that the production of IL-2, IL-6, and IL-13 changed in pristane-induced SLE mice (Figure 2). IL-2 production of splenocytes from mice in model control group was lower than that from normal mice (P < .05), while IL-6 and IL-13 production of splenocytes from mice in model control group was higher than that from normal mice (P < .01). In MT2 and MT3 groups, IL-2 levels were up to the normal level and higher than that of model mice obviously (P < .05), while the IL-6 and IL-13 levels were lower than that of the model mice obviously (P < .05, .01) (Figure 2).

Bottom Line: The results showed that melatonin antagonized the increasing levels of IgM anti ssDNA and histone autoantibodies.Melatonin could also decrease the IL-6 and IL-13 production and increase the IL-2 production.These results suggested that melatonin has a beneficial effect on pristane-induced lupus through regulating the cytokines disturbances.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Anti-inflammatory and immunopharmacology in Education Ministry of China, Institute of Clinical Pharmacology, Anhui Medical University, Hefei 230032, China.

ABSTRACT
Systemic lupus erythematosus (SLE) develops in relation to many environmental factors. In our opinion, it is more important to investigate the effect of melatonin on the environmental- related SLE. In the present study, 0.5 ml pristane were used to induce SLE in female BALB/c mice. Melatonin (0.01, 0.1, 1.0 mg/kg) was orally administered immediately after pristane-injection for 24 weeks. IgM anti ssDNA and histone antibodies were detected after 0, 1, 2, 4, 8 weeks pristane injection. The levels of IL-2, IL-6 and IL-13 were detected after 24 weeks. Renal lesions were also observed. The results showed that melatonin antagonized the increasing levels of IgM anti ssDNA and histone autoantibodies. Melatonin could also decrease the IL-6 and IL-13 production and increase the IL-2 production. Besides, melatonin could lessen the renal lesions caused by pristane. These results suggested that melatonin has a beneficial effect on pristane-induced lupus through regulating the cytokines disturbances.

Show MeSH
Related in: MedlinePlus