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Regulatory effect of melatonin on cytokine disturbances in the pristane-induced lupus mice.

Zhou LL, Wei W, Si JF, Yuan DP - Mediators Inflamm. (2010)

Bottom Line: The results showed that melatonin antagonized the increasing levels of IgM anti ssDNA and histone autoantibodies.Melatonin could also decrease the IL-6 and IL-13 production and increase the IL-2 production.These results suggested that melatonin has a beneficial effect on pristane-induced lupus through regulating the cytokines disturbances.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Anti-inflammatory and immunopharmacology in Education Ministry of China, Institute of Clinical Pharmacology, Anhui Medical University, Hefei 230032, China.

ABSTRACT
Systemic lupus erythematosus (SLE) develops in relation to many environmental factors. In our opinion, it is more important to investigate the effect of melatonin on the environmental- related SLE. In the present study, 0.5 ml pristane were used to induce SLE in female BALB/c mice. Melatonin (0.01, 0.1, 1.0 mg/kg) was orally administered immediately after pristane-injection for 24 weeks. IgM anti ssDNA and histone antibodies were detected after 0, 1, 2, 4, 8 weeks pristane injection. The levels of IL-2, IL-6 and IL-13 were detected after 24 weeks. Renal lesions were also observed. The results showed that melatonin antagonized the increasing levels of IgM anti ssDNA and histone autoantibodies. Melatonin could also decrease the IL-6 and IL-13 production and increase the IL-2 production. Besides, melatonin could lessen the renal lesions caused by pristane. These results suggested that melatonin has a beneficial effect on pristane-induced lupus through regulating the cytokines disturbances.

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Related in: MedlinePlus

The sera of mice in each group were collected before treatment (0), 2, 4, and 8 weeks later, and levels of IgM anti-ssDNA and Antihistone antibodies were detected by ELISA. EI was calculated according to the formula. (a) the level of IgM anti-ssDNA antibody in each group, (b) the level of IgM Antihistone antibody in each group. Data were given in mean ± SD (n = 6–8). *P < .05, **P < .01 versus sera at 0 wk, ∆P < .05, ∆∆P < .01 versus sera in model control mice.
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Related In: Results  -  Collection


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fig1: The sera of mice in each group were collected before treatment (0), 2, 4, and 8 weeks later, and levels of IgM anti-ssDNA and Antihistone antibodies were detected by ELISA. EI was calculated according to the formula. (a) the level of IgM anti-ssDNA antibody in each group, (b) the level of IgM Antihistone antibody in each group. Data were given in mean ± SD (n = 6–8). *P < .05, **P < .01 versus sera at 0 wk, ∆P < .05, ∆∆P < .01 versus sera in model control mice.

Mentions: The levels of IgM anti-ssDNA and histone antibodies were significantly different between pristane-injection and melatonin treatment groups (P < .05, Figures 1(a) and 1(b)).


Regulatory effect of melatonin on cytokine disturbances in the pristane-induced lupus mice.

Zhou LL, Wei W, Si JF, Yuan DP - Mediators Inflamm. (2010)

The sera of mice in each group were collected before treatment (0), 2, 4, and 8 weeks later, and levels of IgM anti-ssDNA and Antihistone antibodies were detected by ELISA. EI was calculated according to the formula. (a) the level of IgM anti-ssDNA antibody in each group, (b) the level of IgM Antihistone antibody in each group. Data were given in mean ± SD (n = 6–8). *P < .05, **P < .01 versus sera at 0 wk, ∆P < .05, ∆∆P < .01 versus sera in model control mice.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2913856&req=5

fig1: The sera of mice in each group were collected before treatment (0), 2, 4, and 8 weeks later, and levels of IgM anti-ssDNA and Antihistone antibodies were detected by ELISA. EI was calculated according to the formula. (a) the level of IgM anti-ssDNA antibody in each group, (b) the level of IgM Antihistone antibody in each group. Data were given in mean ± SD (n = 6–8). *P < .05, **P < .01 versus sera at 0 wk, ∆P < .05, ∆∆P < .01 versus sera in model control mice.
Mentions: The levels of IgM anti-ssDNA and histone antibodies were significantly different between pristane-injection and melatonin treatment groups (P < .05, Figures 1(a) and 1(b)).

Bottom Line: The results showed that melatonin antagonized the increasing levels of IgM anti ssDNA and histone autoantibodies.Melatonin could also decrease the IL-6 and IL-13 production and increase the IL-2 production.These results suggested that melatonin has a beneficial effect on pristane-induced lupus through regulating the cytokines disturbances.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Anti-inflammatory and immunopharmacology in Education Ministry of China, Institute of Clinical Pharmacology, Anhui Medical University, Hefei 230032, China.

ABSTRACT
Systemic lupus erythematosus (SLE) develops in relation to many environmental factors. In our opinion, it is more important to investigate the effect of melatonin on the environmental- related SLE. In the present study, 0.5 ml pristane were used to induce SLE in female BALB/c mice. Melatonin (0.01, 0.1, 1.0 mg/kg) was orally administered immediately after pristane-injection for 24 weeks. IgM anti ssDNA and histone antibodies were detected after 0, 1, 2, 4, 8 weeks pristane injection. The levels of IL-2, IL-6 and IL-13 were detected after 24 weeks. Renal lesions were also observed. The results showed that melatonin antagonized the increasing levels of IgM anti ssDNA and histone autoantibodies. Melatonin could also decrease the IL-6 and IL-13 production and increase the IL-2 production. Besides, melatonin could lessen the renal lesions caused by pristane. These results suggested that melatonin has a beneficial effect on pristane-induced lupus through regulating the cytokines disturbances.

Show MeSH
Related in: MedlinePlus