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Effects of assisted reproduction technology on placental imprinted gene expression.

Katagiri Y, Aoki C, Tamaki-Ishihara Y, Fukuda Y, Kitamura M, Matsue Y, So A, Morita M - Obstet Gynecol Int (2010)

Bottom Line: Of 989 singletons examined (ART n = 65; SC n = 924), neonatal weight was significantly lower (P < .001) in the ART group than in the SC group, but placental weight showed no significant difference.Gene expression analyzed by real-time PCR was similar for both groups with appropriate-for-date (AFD) birth weight.As imprinted gene expression patterns differed between the ART and SC groups, we speculate that ART modifies epigenetic status even though the possibilities always exist.

View Article: PubMed Central - PubMed

Affiliation: Reproduction Center, Department of Obstetrics and Gynecology, Toho University Omori Medical Center, 6-11-1 Omori-Nishi, Ota-ku, Tokyo 143-8541, Japan.

ABSTRACT
We used placental tissue to compare the imprinted gene expression of IGF2, H19, KCNQ1OT1, and CDKN1C of singletons conceived via assisted reproduction technology (ART) with that of spontaneously conceived (SC) singletons. Of 989 singletons examined (ART n = 65; SC n = 924), neonatal weight was significantly lower (P < .001) in the ART group than in the SC group, but placental weight showed no significant difference. Gene expression analyzed by real-time PCR was similar for both groups with appropriate-for-date (AFD) birth weight. H19 expression was suppressed in fetal growth retardation (FGR) cases in the ART and SC groups compared with AFD cases (P < .02 and P < .05, resp.). In contrast, CDKN1C expression was suppressed in FGR cases in the ART group (P < .01), while KCNQ1OT1 expression was hyperexpressed in FGR cases in the SC group (P < .05). As imprinted gene expression patterns differed between the ART and SC groups, we speculate that ART modifies epigenetic status even though the possibilities always exist.

No MeSH data available.


Related in: MedlinePlus

Gene expression in placental tissue. FGR and birth weight ≥3500 g cases in the SC group. H19 expression was significantly reduced in FGR cases, but significantly enhanced in cases with a birth weight of ≥3500 g (P < .01).
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fig3: Gene expression in placental tissue. FGR and birth weight ≥3500 g cases in the SC group. H19 expression was significantly reduced in FGR cases, but significantly enhanced in cases with a birth weight of ≥3500 g (P < .01).

Mentions: The mean birth weight was significantly lower (P < .001) in the ART group (2905.1 ± 459.0 g) than in the SC group (3607.9 ± 589.9 g). The mean placental weight, however, showed no significant difference (ART = 689.3 ± 152.6 g; SC = 613.0 ± 142.5 g) (Table 3). Gene expression patterns in the AFD birth weight cases were similar in both the ART and SC groups (Figure 1). H19 expression was reduced in FGR cases both in the ART and SC groups compared with the AFD cases (P < .02 and P < .05, resp.) (Figure 2). Conversely, H19 expression was significantly enhanced in SC cases with a birth weight of ≥3500 g (P < .01) (Figure 3). On the other hand, CDKN1C expression was reduced in ART cases with FGR (P < .01), and KCNQ1OT1 appeared to be hyperexpressed in SC cases with FGR (P <  .05) (Figure 2). The expression of other genes examined showed no difference from the control.


Effects of assisted reproduction technology on placental imprinted gene expression.

Katagiri Y, Aoki C, Tamaki-Ishihara Y, Fukuda Y, Kitamura M, Matsue Y, So A, Morita M - Obstet Gynecol Int (2010)

Gene expression in placental tissue. FGR and birth weight ≥3500 g cases in the SC group. H19 expression was significantly reduced in FGR cases, but significantly enhanced in cases with a birth weight of ≥3500 g (P < .01).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2913850&req=5

fig3: Gene expression in placental tissue. FGR and birth weight ≥3500 g cases in the SC group. H19 expression was significantly reduced in FGR cases, but significantly enhanced in cases with a birth weight of ≥3500 g (P < .01).
Mentions: The mean birth weight was significantly lower (P < .001) in the ART group (2905.1 ± 459.0 g) than in the SC group (3607.9 ± 589.9 g). The mean placental weight, however, showed no significant difference (ART = 689.3 ± 152.6 g; SC = 613.0 ± 142.5 g) (Table 3). Gene expression patterns in the AFD birth weight cases were similar in both the ART and SC groups (Figure 1). H19 expression was reduced in FGR cases both in the ART and SC groups compared with the AFD cases (P < .02 and P < .05, resp.) (Figure 2). Conversely, H19 expression was significantly enhanced in SC cases with a birth weight of ≥3500 g (P < .01) (Figure 3). On the other hand, CDKN1C expression was reduced in ART cases with FGR (P < .01), and KCNQ1OT1 appeared to be hyperexpressed in SC cases with FGR (P <  .05) (Figure 2). The expression of other genes examined showed no difference from the control.

Bottom Line: Of 989 singletons examined (ART n = 65; SC n = 924), neonatal weight was significantly lower (P < .001) in the ART group than in the SC group, but placental weight showed no significant difference.Gene expression analyzed by real-time PCR was similar for both groups with appropriate-for-date (AFD) birth weight.As imprinted gene expression patterns differed between the ART and SC groups, we speculate that ART modifies epigenetic status even though the possibilities always exist.

View Article: PubMed Central - PubMed

Affiliation: Reproduction Center, Department of Obstetrics and Gynecology, Toho University Omori Medical Center, 6-11-1 Omori-Nishi, Ota-ku, Tokyo 143-8541, Japan.

ABSTRACT
We used placental tissue to compare the imprinted gene expression of IGF2, H19, KCNQ1OT1, and CDKN1C of singletons conceived via assisted reproduction technology (ART) with that of spontaneously conceived (SC) singletons. Of 989 singletons examined (ART n = 65; SC n = 924), neonatal weight was significantly lower (P < .001) in the ART group than in the SC group, but placental weight showed no significant difference. Gene expression analyzed by real-time PCR was similar for both groups with appropriate-for-date (AFD) birth weight. H19 expression was suppressed in fetal growth retardation (FGR) cases in the ART and SC groups compared with AFD cases (P < .02 and P < .05, resp.). In contrast, CDKN1C expression was suppressed in FGR cases in the ART group (P < .01), while KCNQ1OT1 expression was hyperexpressed in FGR cases in the SC group (P < .05). As imprinted gene expression patterns differed between the ART and SC groups, we speculate that ART modifies epigenetic status even though the possibilities always exist.

No MeSH data available.


Related in: MedlinePlus