Limits...
Electrodelivery of drugs into cancer cells in the presence of poloxamer 188.

Tsoneva I, Iordanov I, Berger AJ, Tomov T, Nikolova B, Mudrov N, Berger MR - J. Biomed. Biotechnol. (2010)

Bottom Line: In the present study it is shown that poloxamer 188, added before or immediately after an electrical pulse used for electroporation, decreases the number of dead cells and at the same time does not reduce the number of reversible electropores through which small molecules (cisplatin, bleomycin, or propidium iodide) can pass/diffuse.It was suggested that hydrophobic sections of poloxamer 188 molecules are incorporated into the edges of pores and that their hydrophilic parts act as brushy pore structures.The formation of brushy pores may reduce the expansion of pores and delay the irreversible electropermeability.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biophysics, Bulgarian Academy of Sciences, Sofia, Bulgaria. itsoneva@obzor.bio21.bas.bg

ABSTRACT
In the present study it is shown that poloxamer 188, added before or immediately after an electrical pulse used for electroporation, decreases the number of dead cells and at the same time does not reduce the number of reversible electropores through which small molecules (cisplatin, bleomycin, or propidium iodide) can pass/diffuse. It was suggested that hydrophobic sections of poloxamer 188 molecules are incorporated into the edges of pores and that their hydrophilic parts act as brushy pore structures. The formation of brushy pores may reduce the expansion of pores and delay the irreversible electropermeability. Tumors were implanted subcutaneously in both flanks of nude mice using HeLa cells, transfected with genes for red fluorescent protein and luciferase. The volume of tumors stopped to grow after electrochemotherapy and the use of poloxamer 188 reduced the edema near the electrode and around the subcutaneously growing tumors.

Show MeSH

Related in: MedlinePlus

Scheme on the mechanism of P188 interaction in biological membrane (explanation is given in the text).
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2913842&req=5

fig9: Scheme on the mechanism of P188 interaction in biological membrane (explanation is given in the text).

Mentions: On the basis of the experimental results (Figures 1–5) we suggest the following mechanism of action of triblock copolymer (Figure 9).


Electrodelivery of drugs into cancer cells in the presence of poloxamer 188.

Tsoneva I, Iordanov I, Berger AJ, Tomov T, Nikolova B, Mudrov N, Berger MR - J. Biomed. Biotechnol. (2010)

Scheme on the mechanism of P188 interaction in biological membrane (explanation is given in the text).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2913842&req=5

fig9: Scheme on the mechanism of P188 interaction in biological membrane (explanation is given in the text).
Mentions: On the basis of the experimental results (Figures 1–5) we suggest the following mechanism of action of triblock copolymer (Figure 9).

Bottom Line: In the present study it is shown that poloxamer 188, added before or immediately after an electrical pulse used for electroporation, decreases the number of dead cells and at the same time does not reduce the number of reversible electropores through which small molecules (cisplatin, bleomycin, or propidium iodide) can pass/diffuse.It was suggested that hydrophobic sections of poloxamer 188 molecules are incorporated into the edges of pores and that their hydrophilic parts act as brushy pore structures.The formation of brushy pores may reduce the expansion of pores and delay the irreversible electropermeability.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biophysics, Bulgarian Academy of Sciences, Sofia, Bulgaria. itsoneva@obzor.bio21.bas.bg

ABSTRACT
In the present study it is shown that poloxamer 188, added before or immediately after an electrical pulse used for electroporation, decreases the number of dead cells and at the same time does not reduce the number of reversible electropores through which small molecules (cisplatin, bleomycin, or propidium iodide) can pass/diffuse. It was suggested that hydrophobic sections of poloxamer 188 molecules are incorporated into the edges of pores and that their hydrophilic parts act as brushy pore structures. The formation of brushy pores may reduce the expansion of pores and delay the irreversible electropermeability. Tumors were implanted subcutaneously in both flanks of nude mice using HeLa cells, transfected with genes for red fluorescent protein and luciferase. The volume of tumors stopped to grow after electrochemotherapy and the use of poloxamer 188 reduced the edema near the electrode and around the subcutaneously growing tumors.

Show MeSH
Related in: MedlinePlus