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Electrodelivery of drugs into cancer cells in the presence of poloxamer 188.

Tsoneva I, Iordanov I, Berger AJ, Tomov T, Nikolova B, Mudrov N, Berger MR - J. Biomed. Biotechnol. (2010)

Bottom Line: In the present study it is shown that poloxamer 188, added before or immediately after an electrical pulse used for electroporation, decreases the number of dead cells and at the same time does not reduce the number of reversible electropores through which small molecules (cisplatin, bleomycin, or propidium iodide) can pass/diffuse.It was suggested that hydrophobic sections of poloxamer 188 molecules are incorporated into the edges of pores and that their hydrophilic parts act as brushy pore structures.The formation of brushy pores may reduce the expansion of pores and delay the irreversible electropermeability.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biophysics, Bulgarian Academy of Sciences, Sofia, Bulgaria. itsoneva@obzor.bio21.bas.bg

ABSTRACT
In the present study it is shown that poloxamer 188, added before or immediately after an electrical pulse used for electroporation, decreases the number of dead cells and at the same time does not reduce the number of reversible electropores through which small molecules (cisplatin, bleomycin, or propidium iodide) can pass/diffuse. It was suggested that hydrophobic sections of poloxamer 188 molecules are incorporated into the edges of pores and that their hydrophilic parts act as brushy pore structures. The formation of brushy pores may reduce the expansion of pores and delay the irreversible electropermeability. Tumors were implanted subcutaneously in both flanks of nude mice using HeLa cells, transfected with genes for red fluorescent protein and luciferase. The volume of tumors stopped to grow after electrochemotherapy and the use of poloxamer 188 reduced the edema near the electrode and around the subcutaneously growing tumors.

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Growth curves of HeLa tumors implanted in Nude mice: control (without any treatment); chemotherapy with cisplatin; electrochemotherapy with cisplatin injected intratumorally, electrochemotherapy in the presence of P188 injected 5 minutes after pulses intraperitoneally. The observed time was 30 days. Points are mean value of three tumors. Standard errors are omitted due to the overlap of the bars.
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fig7: Growth curves of HeLa tumors implanted in Nude mice: control (without any treatment); chemotherapy with cisplatin; electrochemotherapy with cisplatin injected intratumorally, electrochemotherapy in the presence of P188 injected 5 minutes after pulses intraperitoneally. The observed time was 30 days. Points are mean value of three tumors. Standard errors are omitted due to the overlap of the bars.

Mentions: The changes in tumor growth after the various treatment arms are shown in Figure 7. Chemotherapy with cisplatin reduced the tumor volume by 50% compared with untreated controls (not significant). The electrotreatment modes (electrochemotherapy or electrochemotherapy + P188) stopped the tumor growth within the observation period of 30 days. This corresponds to a tumor growth inhibition of more than 90% compared to untreated controls and is highly significant. The differences in tumor growth were analysed by the Kruskal Wallis test and found significant for the two electroporation groups.


Electrodelivery of drugs into cancer cells in the presence of poloxamer 188.

Tsoneva I, Iordanov I, Berger AJ, Tomov T, Nikolova B, Mudrov N, Berger MR - J. Biomed. Biotechnol. (2010)

Growth curves of HeLa tumors implanted in Nude mice: control (without any treatment); chemotherapy with cisplatin; electrochemotherapy with cisplatin injected intratumorally, electrochemotherapy in the presence of P188 injected 5 minutes after pulses intraperitoneally. The observed time was 30 days. Points are mean value of three tumors. Standard errors are omitted due to the overlap of the bars.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2913842&req=5

fig7: Growth curves of HeLa tumors implanted in Nude mice: control (without any treatment); chemotherapy with cisplatin; electrochemotherapy with cisplatin injected intratumorally, electrochemotherapy in the presence of P188 injected 5 minutes after pulses intraperitoneally. The observed time was 30 days. Points are mean value of three tumors. Standard errors are omitted due to the overlap of the bars.
Mentions: The changes in tumor growth after the various treatment arms are shown in Figure 7. Chemotherapy with cisplatin reduced the tumor volume by 50% compared with untreated controls (not significant). The electrotreatment modes (electrochemotherapy or electrochemotherapy + P188) stopped the tumor growth within the observation period of 30 days. This corresponds to a tumor growth inhibition of more than 90% compared to untreated controls and is highly significant. The differences in tumor growth were analysed by the Kruskal Wallis test and found significant for the two electroporation groups.

Bottom Line: In the present study it is shown that poloxamer 188, added before or immediately after an electrical pulse used for electroporation, decreases the number of dead cells and at the same time does not reduce the number of reversible electropores through which small molecules (cisplatin, bleomycin, or propidium iodide) can pass/diffuse.It was suggested that hydrophobic sections of poloxamer 188 molecules are incorporated into the edges of pores and that their hydrophilic parts act as brushy pore structures.The formation of brushy pores may reduce the expansion of pores and delay the irreversible electropermeability.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biophysics, Bulgarian Academy of Sciences, Sofia, Bulgaria. itsoneva@obzor.bio21.bas.bg

ABSTRACT
In the present study it is shown that poloxamer 188, added before or immediately after an electrical pulse used for electroporation, decreases the number of dead cells and at the same time does not reduce the number of reversible electropores through which small molecules (cisplatin, bleomycin, or propidium iodide) can pass/diffuse. It was suggested that hydrophobic sections of poloxamer 188 molecules are incorporated into the edges of pores and that their hydrophilic parts act as brushy pore structures. The formation of brushy pores may reduce the expansion of pores and delay the irreversible electropermeability. Tumors were implanted subcutaneously in both flanks of nude mice using HeLa cells, transfected with genes for red fluorescent protein and luciferase. The volume of tumors stopped to grow after electrochemotherapy and the use of poloxamer 188 reduced the edema near the electrode and around the subcutaneously growing tumors.

Show MeSH
Related in: MedlinePlus