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Electrodelivery of drugs into cancer cells in the presence of poloxamer 188.

Tsoneva I, Iordanov I, Berger AJ, Tomov T, Nikolova B, Mudrov N, Berger MR - J. Biomed. Biotechnol. (2010)

Bottom Line: In the present study it is shown that poloxamer 188, added before or immediately after an electrical pulse used for electroporation, decreases the number of dead cells and at the same time does not reduce the number of reversible electropores through which small molecules (cisplatin, bleomycin, or propidium iodide) can pass/diffuse.It was suggested that hydrophobic sections of poloxamer 188 molecules are incorporated into the edges of pores and that their hydrophilic parts act as brushy pore structures.The formation of brushy pores may reduce the expansion of pores and delay the irreversible electropermeability.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biophysics, Bulgarian Academy of Sciences, Sofia, Bulgaria. itsoneva@obzor.bio21.bas.bg

ABSTRACT
In the present study it is shown that poloxamer 188, added before or immediately after an electrical pulse used for electroporation, decreases the number of dead cells and at the same time does not reduce the number of reversible electropores through which small molecules (cisplatin, bleomycin, or propidium iodide) can pass/diffuse. It was suggested that hydrophobic sections of poloxamer 188 molecules are incorporated into the edges of pores and that their hydrophilic parts act as brushy pore structures. The formation of brushy pores may reduce the expansion of pores and delay the irreversible electropermeability. Tumors were implanted subcutaneously in both flanks of nude mice using HeLa cells, transfected with genes for red fluorescent protein and luciferase. The volume of tumors stopped to grow after electrochemotherapy and the use of poloxamer 188 reduced the edema near the electrode and around the subcutaneously growing tumors.

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Related in: MedlinePlus

Haemolysis of erythrocytes, electroporated in the presence of different concentrations (μM) of P188 after 3 hours. The electrical parameters were the same as in Figure 5. The abscissa is in logarithmic scale, the point 0.01 corresponds to the control (zero concentration of P188).
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fig6: Haemolysis of erythrocytes, electroporated in the presence of different concentrations (μM) of P188 after 3 hours. The electrical parameters were the same as in Figure 5. The abscissa is in logarithmic scale, the point 0.01 corresponds to the control (zero concentration of P188).

Mentions: The dependence of electrohaemolysis on the concentrations of P188 after 3 hours is given in Figure 6. Experimental conditions for the electroporation (field strength of 3 kV · cm−1 and 1 pulse with a duration of 0.5 ms) were such that the extent of the irreversibly damaged erythrocytes was high. The number of electrohaemolysed (irreversibly electroporated) cells was reduced with the increase in the concentration of P188.


Electrodelivery of drugs into cancer cells in the presence of poloxamer 188.

Tsoneva I, Iordanov I, Berger AJ, Tomov T, Nikolova B, Mudrov N, Berger MR - J. Biomed. Biotechnol. (2010)

Haemolysis of erythrocytes, electroporated in the presence of different concentrations (μM) of P188 after 3 hours. The electrical parameters were the same as in Figure 5. The abscissa is in logarithmic scale, the point 0.01 corresponds to the control (zero concentration of P188).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2913842&req=5

fig6: Haemolysis of erythrocytes, electroporated in the presence of different concentrations (μM) of P188 after 3 hours. The electrical parameters were the same as in Figure 5. The abscissa is in logarithmic scale, the point 0.01 corresponds to the control (zero concentration of P188).
Mentions: The dependence of electrohaemolysis on the concentrations of P188 after 3 hours is given in Figure 6. Experimental conditions for the electroporation (field strength of 3 kV · cm−1 and 1 pulse with a duration of 0.5 ms) were such that the extent of the irreversibly damaged erythrocytes was high. The number of electrohaemolysed (irreversibly electroporated) cells was reduced with the increase in the concentration of P188.

Bottom Line: In the present study it is shown that poloxamer 188, added before or immediately after an electrical pulse used for electroporation, decreases the number of dead cells and at the same time does not reduce the number of reversible electropores through which small molecules (cisplatin, bleomycin, or propidium iodide) can pass/diffuse.It was suggested that hydrophobic sections of poloxamer 188 molecules are incorporated into the edges of pores and that their hydrophilic parts act as brushy pore structures.The formation of brushy pores may reduce the expansion of pores and delay the irreversible electropermeability.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biophysics, Bulgarian Academy of Sciences, Sofia, Bulgaria. itsoneva@obzor.bio21.bas.bg

ABSTRACT
In the present study it is shown that poloxamer 188, added before or immediately after an electrical pulse used for electroporation, decreases the number of dead cells and at the same time does not reduce the number of reversible electropores through which small molecules (cisplatin, bleomycin, or propidium iodide) can pass/diffuse. It was suggested that hydrophobic sections of poloxamer 188 molecules are incorporated into the edges of pores and that their hydrophilic parts act as brushy pore structures. The formation of brushy pores may reduce the expansion of pores and delay the irreversible electropermeability. Tumors were implanted subcutaneously in both flanks of nude mice using HeLa cells, transfected with genes for red fluorescent protein and luciferase. The volume of tumors stopped to grow after electrochemotherapy and the use of poloxamer 188 reduced the edema near the electrode and around the subcutaneously growing tumors.

Show MeSH
Related in: MedlinePlus