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Rank Protein Immunolabeling during Bone-Implant Interface Healing Process.

Avila Souza F, Pereira Queiroz T, Rodrigues Luvizuto E, Nishioka RS, Garcia-Jr IR, de Carvalho PS, Okamoto R - Int J Dent (2010)

Bottom Line: Results.The statistical analysis showed an increase in the expression of RANK in osteoblasts at 7 postoperative days and a gradual decrease during the chronology of the healing process demonstrated by mild cellular activity in the final stage (P < .05).Conclusion.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery and General Clinical, Araçatuba Dental School, Universidade Estadual Paulista, UNESP, Rua José Bonifácio 1190, Bloco 10A, Vila Mendonça, 16015-050 Araçatuba, Brazil.

ABSTRACT
The purpose of this paper was to evaluate the expression of RANK protein during bone-healing process around machined surface implants. Twenty male Wistar rats, 90 days old, after having had a 2 mm diameter and 6 mm long implant inserted in their right tibias, were evaluated at 7, 14, 21, and 42 days after healing. After obtaining the histological samples, slides were subjected to RANK immunostaining reaction. Results were quantitatively evaluated. Results. Immunolabeling analysis showed expressions of RANK in osteoclast and osteoblast lineage cells. The statistical analysis showed an increase in the expression of RANK in osteoblasts at 7 postoperative days and a gradual decrease during the chronology of the healing process demonstrated by mild cellular activity in the final stage (P < .05). Conclusion. RANK immunolabeling was observed especially in osteoclast and osteoblast cells in primary bone during the initial periods of bone-healing/implant interface.

No MeSH data available.


Related in: MedlinePlus

Positive controls from RANK expression. (a) Rats nasal cavity showing RANK expression in preosteoclasts lineage cells. (b) Rat embryo with 21 days of pregnancy. A bodily structure that expresses just osteoblasts lineage cells in primary bone tissue is the palatine process.
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fig3: Positive controls from RANK expression. (a) Rats nasal cavity showing RANK expression in preosteoclasts lineage cells. (b) Rat embryo with 21 days of pregnancy. A bodily structure that expresses just osteoblasts lineage cells in primary bone tissue is the palatine process.

Mentions: In addition, to evaluate the possibility of RANK protein expression in osteoblast-lineage cells, immunohistochemical reactions were performed in the rat embryo at 21 days of pregnancy. A bodily structure that expresses only osteoblast-lineage cells in primary bone tissue is the palatine process. This process was also analyzed as a positive control and showed positive immunolabeling for RANK protein (Figure 3). The immunomarkings shown in Image 3 refer to the RANK immunomarkings in areas outside of the studied area, functioning as positive control to show the effectiveness of the antibody used. Figure 3(a) shows the RANK expression in osteoclasts of the nasal mucosa of rats, and Figure 3(b) shows the RANK expression in osteoblasts in the primary bone tissue of rat embryos. These two regions outside of the studied area are regions that are known to have a large quantity of osteoclasts in the nasal cavity and a large quantity of osteoblasts in rat embryos. Therefore, the specificity of the antibody used is confirmed.


Rank Protein Immunolabeling during Bone-Implant Interface Healing Process.

Avila Souza F, Pereira Queiroz T, Rodrigues Luvizuto E, Nishioka RS, Garcia-Jr IR, de Carvalho PS, Okamoto R - Int J Dent (2010)

Positive controls from RANK expression. (a) Rats nasal cavity showing RANK expression in preosteoclasts lineage cells. (b) Rat embryo with 21 days of pregnancy. A bodily structure that expresses just osteoblasts lineage cells in primary bone tissue is the palatine process.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2913635&req=5

fig3: Positive controls from RANK expression. (a) Rats nasal cavity showing RANK expression in preosteoclasts lineage cells. (b) Rat embryo with 21 days of pregnancy. A bodily structure that expresses just osteoblasts lineage cells in primary bone tissue is the palatine process.
Mentions: In addition, to evaluate the possibility of RANK protein expression in osteoblast-lineage cells, immunohistochemical reactions were performed in the rat embryo at 21 days of pregnancy. A bodily structure that expresses only osteoblast-lineage cells in primary bone tissue is the palatine process. This process was also analyzed as a positive control and showed positive immunolabeling for RANK protein (Figure 3). The immunomarkings shown in Image 3 refer to the RANK immunomarkings in areas outside of the studied area, functioning as positive control to show the effectiveness of the antibody used. Figure 3(a) shows the RANK expression in osteoclasts of the nasal mucosa of rats, and Figure 3(b) shows the RANK expression in osteoblasts in the primary bone tissue of rat embryos. These two regions outside of the studied area are regions that are known to have a large quantity of osteoclasts in the nasal cavity and a large quantity of osteoblasts in rat embryos. Therefore, the specificity of the antibody used is confirmed.

Bottom Line: Results.The statistical analysis showed an increase in the expression of RANK in osteoblasts at 7 postoperative days and a gradual decrease during the chronology of the healing process demonstrated by mild cellular activity in the final stage (P < .05).Conclusion.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery and General Clinical, Araçatuba Dental School, Universidade Estadual Paulista, UNESP, Rua José Bonifácio 1190, Bloco 10A, Vila Mendonça, 16015-050 Araçatuba, Brazil.

ABSTRACT
The purpose of this paper was to evaluate the expression of RANK protein during bone-healing process around machined surface implants. Twenty male Wistar rats, 90 days old, after having had a 2 mm diameter and 6 mm long implant inserted in their right tibias, were evaluated at 7, 14, 21, and 42 days after healing. After obtaining the histological samples, slides were subjected to RANK immunostaining reaction. Results were quantitatively evaluated. Results. Immunolabeling analysis showed expressions of RANK in osteoclast and osteoblast lineage cells. The statistical analysis showed an increase in the expression of RANK in osteoblasts at 7 postoperative days and a gradual decrease during the chronology of the healing process demonstrated by mild cellular activity in the final stage (P < .05). Conclusion. RANK immunolabeling was observed especially in osteoclast and osteoblast cells in primary bone during the initial periods of bone-healing/implant interface.

No MeSH data available.


Related in: MedlinePlus