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Elevated levels of IL-10 and G-CSF associated with asymptomatic malaria in pregnant women.

Wilson NO, Bythwood T, Solomon W, Jolly P, Yatich N, Jiang Y, Shuaib F, Adjei AA, Anderson W, Stiles JK - Infect Dis Obstet Gynecol (2010)

Bottom Line: Plasma from healthy (n = 15) and asymptomatic (n = 25) pregnant women were evaluated for 27 biomarkers (IL-1b, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12, IL-13, IL-15, IL- 17, Eotaxin, bFGF-2, G-CSF, GM-CSF, IFN-gamma, IP-10, MCP-1, MIP-1alpha, MIP-1beta, PDGF-bb, RANTES, TNF, and VEGF) associated with Th1 and Th2 cytokine homeostasis.IL-10 and G-CSF levels were elevated in the asymptomatic group when compared with the healthy group (P = .031 and .041, resp.).The median ratios of IL-1beta:5, IL-1beta:10, IL-1beta:G-CSF, IL-1beta:Eotaxin, IL-12:G-CSF, IL-15:10, IL-17:G-CSF, IL-17:Eotaxin, TNF:IL-4, TNF:IL-5, and TNF:G-CSF were significantly different among the two groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Biochemistry & Immunology, Morehouse School of Medicine, Atlanta, GA 30310, USA.

ABSTRACT
In sub-Saharan Africa, approximately 30 million pregnant women are at risk of contracting malaria annually. Nearly 36% of healthy pregnant women receiving routine antenatal care tested positive for Plasmodium falciparum HRP-II antigen in Ghana. We tested the hypothesis that asymptomatic HRP II positive pregnant women expressed a unique Th1 and Th2 phenotype that differs from healthy controls. Plasma from healthy (n = 15) and asymptomatic (n = 25) pregnant women were evaluated for 27 biomarkers (IL-1b, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12, IL-13, IL-15, IL- 17, Eotaxin, bFGF-2, G-CSF, GM-CSF, IFN-gamma, IP-10, MCP-1, MIP-1alpha, MIP-1beta, PDGF-bb, RANTES, TNF, and VEGF) associated with Th1 and Th2 cytokine homeostasis. IL-10 and G-CSF levels were elevated in the asymptomatic group when compared with the healthy group (P = .031 and .041, resp.). The median ratios of IL-1beta:5, IL-1beta:10, IL-1beta:G-CSF, IL-1beta:Eotaxin, IL-12:G-CSF, IL-15:10, IL-17:G-CSF, IL-17:Eotaxin, TNF:IL-4, TNF:IL-5, and TNF:G-CSF were significantly different among the two groups. Thus, asymptomatic malaria carriage may be linked to circulating levels of IL-10 and G-CSF.

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Related in: MedlinePlus

Concentration of plasma IL-10 and G-CSF in pregnant women with asymptomatic malaria (n = 25) and healthy controls (n = 15). Box plots represent median log concentration (pg/ml) and 25th and 75th percentiles. Outliers are shown as points outside the 10th and 90th percentile bars. HC represents healthy controls. AC denotes asymptomatic carriers. *P < .05 is statistically significant.
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fig1: Concentration of plasma IL-10 and G-CSF in pregnant women with asymptomatic malaria (n = 25) and healthy controls (n = 15). Box plots represent median log concentration (pg/ml) and 25th and 75th percentiles. Outliers are shown as points outside the 10th and 90th percentile bars. HC represents healthy controls. AC denotes asymptomatic carriers. *P < .05 is statistically significant.

Mentions: Pairwise comparisons were used to determine the levels of significance of the differences between the plasma biological factor levels of the two groups (HC and AC) after controlling for age and primigravidae. The plasma levels of 27 biological factors were grouped into proinflammatory, anti-inflammatory, and angiogenic/angiostatic biomarkers and analyzed. The proinflammatory and angiogenic/angiostatic levels demonstrated marginal changes but did not show statistically significant differences between the HC and AC groups (data not shown). Among the anti-inflammatory biomarkers evaluated, IL-10 and G-CSF were significantly (P < .05) elevated among the AC group compared to HC group (Figure 1). In addition, among the AC group comparing helminth infection with absence of helminth infection, there were no significant differences in the peripheral biological factor levels suggesting that helminth infection did not influence the peripheral biological factor levels.


Elevated levels of IL-10 and G-CSF associated with asymptomatic malaria in pregnant women.

Wilson NO, Bythwood T, Solomon W, Jolly P, Yatich N, Jiang Y, Shuaib F, Adjei AA, Anderson W, Stiles JK - Infect Dis Obstet Gynecol (2010)

Concentration of plasma IL-10 and G-CSF in pregnant women with asymptomatic malaria (n = 25) and healthy controls (n = 15). Box plots represent median log concentration (pg/ml) and 25th and 75th percentiles. Outliers are shown as points outside the 10th and 90th percentile bars. HC represents healthy controls. AC denotes asymptomatic carriers. *P < .05 is statistically significant.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2913525&req=5

fig1: Concentration of plasma IL-10 and G-CSF in pregnant women with asymptomatic malaria (n = 25) and healthy controls (n = 15). Box plots represent median log concentration (pg/ml) and 25th and 75th percentiles. Outliers are shown as points outside the 10th and 90th percentile bars. HC represents healthy controls. AC denotes asymptomatic carriers. *P < .05 is statistically significant.
Mentions: Pairwise comparisons were used to determine the levels of significance of the differences between the plasma biological factor levels of the two groups (HC and AC) after controlling for age and primigravidae. The plasma levels of 27 biological factors were grouped into proinflammatory, anti-inflammatory, and angiogenic/angiostatic biomarkers and analyzed. The proinflammatory and angiogenic/angiostatic levels demonstrated marginal changes but did not show statistically significant differences between the HC and AC groups (data not shown). Among the anti-inflammatory biomarkers evaluated, IL-10 and G-CSF were significantly (P < .05) elevated among the AC group compared to HC group (Figure 1). In addition, among the AC group comparing helminth infection with absence of helminth infection, there were no significant differences in the peripheral biological factor levels suggesting that helminth infection did not influence the peripheral biological factor levels.

Bottom Line: Plasma from healthy (n = 15) and asymptomatic (n = 25) pregnant women were evaluated for 27 biomarkers (IL-1b, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12, IL-13, IL-15, IL- 17, Eotaxin, bFGF-2, G-CSF, GM-CSF, IFN-gamma, IP-10, MCP-1, MIP-1alpha, MIP-1beta, PDGF-bb, RANTES, TNF, and VEGF) associated with Th1 and Th2 cytokine homeostasis.IL-10 and G-CSF levels were elevated in the asymptomatic group when compared with the healthy group (P = .031 and .041, resp.).The median ratios of IL-1beta:5, IL-1beta:10, IL-1beta:G-CSF, IL-1beta:Eotaxin, IL-12:G-CSF, IL-15:10, IL-17:G-CSF, IL-17:Eotaxin, TNF:IL-4, TNF:IL-5, and TNF:G-CSF were significantly different among the two groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Biochemistry & Immunology, Morehouse School of Medicine, Atlanta, GA 30310, USA.

ABSTRACT
In sub-Saharan Africa, approximately 30 million pregnant women are at risk of contracting malaria annually. Nearly 36% of healthy pregnant women receiving routine antenatal care tested positive for Plasmodium falciparum HRP-II antigen in Ghana. We tested the hypothesis that asymptomatic HRP II positive pregnant women expressed a unique Th1 and Th2 phenotype that differs from healthy controls. Plasma from healthy (n = 15) and asymptomatic (n = 25) pregnant women were evaluated for 27 biomarkers (IL-1b, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12, IL-13, IL-15, IL- 17, Eotaxin, bFGF-2, G-CSF, GM-CSF, IFN-gamma, IP-10, MCP-1, MIP-1alpha, MIP-1beta, PDGF-bb, RANTES, TNF, and VEGF) associated with Th1 and Th2 cytokine homeostasis. IL-10 and G-CSF levels were elevated in the asymptomatic group when compared with the healthy group (P = .031 and .041, resp.). The median ratios of IL-1beta:5, IL-1beta:10, IL-1beta:G-CSF, IL-1beta:Eotaxin, IL-12:G-CSF, IL-15:10, IL-17:G-CSF, IL-17:Eotaxin, TNF:IL-4, TNF:IL-5, and TNF:G-CSF were significantly different among the two groups. Thus, asymptomatic malaria carriage may be linked to circulating levels of IL-10 and G-CSF.

Show MeSH
Related in: MedlinePlus