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Modelling and experimental analysis of hormonal crosstalk in Arabidopsis.

Liu J, Mehdi S, Topping J, Tarkowski P, Lindsey K - Mol. Syst. Biol. (2010)

Bottom Line: Modelling correctly predicts experimental results for the effect of the pls gene mutation on endogenous cytokinin concentration.Modelling further reveals that a bell-shaped dose-response relationship between endogenous auxin and root length is established via PLS.This combined modelling and experimental analysis provides new insights into the integration of hormonal signals in plants.

View Article: PubMed Central - PubMed

Affiliation: The Integrative Cell Biology Laboratory and The Biophysical Sciences Institute, School of Biological and Biomedical Sciences, Durham University, Durham, UK. junli.liu@durham.ac.uk

ABSTRACT
An important question in plant biology is how genes influence the crosstalk between hormones to regulate growth. In this study, we model POLARIS (PLS) gene function and crosstalk between auxin, ethylene and cytokinin in Arabidopsis. Experimental evidence suggests that PLS acts on or close to the ethylene receptor ETR1, and a mathematical model describing possible PLS-ethylene pathway interactions is developed, and used to make quantitative predictions about PLS-hormone interactions. Modelling correctly predicts experimental results for the effect of the pls gene mutation on endogenous cytokinin concentration. Modelling also reveals a role for PLS in auxin biosynthesis in addition to a role in auxin transport. The model reproduces available mutants, and with new experimental data provides new insights into how PLS regulates auxin concentration, by controlling the relative contribution of auxin transport and biosynthesis and by integrating auxin, ethylene and cytokinin signalling. Modelling further reveals that a bell-shaped dose-response relationship between endogenous auxin and root length is established via PLS. This combined modelling and experimental analysis provides new insights into the integration of hormonal signals in plants.

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Related in: MedlinePlus

Model prediction for endogenous cytokinin concentration in wild type and PLS mutant is in agreement with experimental observations.
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f3: Model prediction for endogenous cytokinin concentration in wild type and PLS mutant is in agreement with experimental observations.

Mentions: Figure 3 predicts that increase in PLS transcription decreases the endogenous cytokinin concentration. It is predicted that in the pls mutant, endogenous cytokinin concentration is increased to 1.48-fold of that in wild type. To experimentally test this prediction, the concentrations of endogenous cytokinins were measured using GC-MS in both pls and wild-type seedlings.


Modelling and experimental analysis of hormonal crosstalk in Arabidopsis.

Liu J, Mehdi S, Topping J, Tarkowski P, Lindsey K - Mol. Syst. Biol. (2010)

Model prediction for endogenous cytokinin concentration in wild type and PLS mutant is in agreement with experimental observations.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2913391&req=5

f3: Model prediction for endogenous cytokinin concentration in wild type and PLS mutant is in agreement with experimental observations.
Mentions: Figure 3 predicts that increase in PLS transcription decreases the endogenous cytokinin concentration. It is predicted that in the pls mutant, endogenous cytokinin concentration is increased to 1.48-fold of that in wild type. To experimentally test this prediction, the concentrations of endogenous cytokinins were measured using GC-MS in both pls and wild-type seedlings.

Bottom Line: Modelling correctly predicts experimental results for the effect of the pls gene mutation on endogenous cytokinin concentration.Modelling further reveals that a bell-shaped dose-response relationship between endogenous auxin and root length is established via PLS.This combined modelling and experimental analysis provides new insights into the integration of hormonal signals in plants.

View Article: PubMed Central - PubMed

Affiliation: The Integrative Cell Biology Laboratory and The Biophysical Sciences Institute, School of Biological and Biomedical Sciences, Durham University, Durham, UK. junli.liu@durham.ac.uk

ABSTRACT
An important question in plant biology is how genes influence the crosstalk between hormones to regulate growth. In this study, we model POLARIS (PLS) gene function and crosstalk between auxin, ethylene and cytokinin in Arabidopsis. Experimental evidence suggests that PLS acts on or close to the ethylene receptor ETR1, and a mathematical model describing possible PLS-ethylene pathway interactions is developed, and used to make quantitative predictions about PLS-hormone interactions. Modelling correctly predicts experimental results for the effect of the pls gene mutation on endogenous cytokinin concentration. Modelling also reveals a role for PLS in auxin biosynthesis in addition to a role in auxin transport. The model reproduces available mutants, and with new experimental data provides new insights into how PLS regulates auxin concentration, by controlling the relative contribution of auxin transport and biosynthesis and by integrating auxin, ethylene and cytokinin signalling. Modelling further reveals that a bell-shaped dose-response relationship between endogenous auxin and root length is established via PLS. This combined modelling and experimental analysis provides new insights into the integration of hormonal signals in plants.

Show MeSH
Related in: MedlinePlus