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Malignant mammary tumor in female dogs: environmental contaminants.

Andrade FH, Figueiroa FC, Bersano PR, Bissacot DZ, Rocha NS - Diagn Pathol (2010)

Bottom Line: Five tumors were positive for estrogen receptors in immunohistochemical analysis.The contamination level was observed in more aggressive tumors.Results suggest the possible involvement of pyrethroid in the canine mammary tumor carcinogenesis.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP) - Botucatu, SP, Brazil. rochanoeme@fmvz.unesp.br

ABSTRACT
Mammary tumors of female dogs have greatly increased in recent years, thus demanding rapid diagnosis and effective treatment in order to determine the animal survival. There is considerable scientific interest in the possible role of environmental contaminants in the etiology of mammary tumors, specifically in relation to synthetic chemical substances released into the environment to which living beings are either directly or indirectly exposed. In this study, the presence of pyrethroid insecticide was observed in adjacent adipose tissue of canine mammary tumor. High Precision Liquid Chromatography - HPLC was adapted to detect and identify environmental contaminants in adipose tissue adjacent to malignant mammary tumor in nine female dogs, without predilection for breed or age. After surgery, masses were carefully examined for malignant neoplastic lesions. Five grams of adipose tissue adjacent to the tumor were collected to detect of environmental contaminants. The identified pyrethroids were allethrin, cyhalothrin, cypermethrin, deltamethrin and tetramethrin, with a contamination level of 33.3%. Histopathology demonstrated six female dogs (66.7%) as having complex carcinoma and three (33.3%) with simple carcinoma. From these tumors, seven (77.8%) presented aggressiveness degree III and two (22.2%) degree I. Five tumors were positive for estrogen receptors in immunohistochemical analysis. The contamination level was observed in more aggressive tumors. This was the first report in which the level of environmental contaminants could be detected in adipose tissue of female dogs with malignant mammary tumor, by HPLC. Results suggest the possible involvement of pyrethroid in the canine mammary tumor carcinogenesis. Hence, the dog may be used as a sentinel animal for human breast cancer, since human beings share the same environment and basically have the same eating habits.

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Simple Carcinoma. Neoplasia composed by proliferated epithelium cells, generating a hard standard with the loss of the glandular architecture.
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Figure 3: Simple Carcinoma. Neoplasia composed by proliferated epithelium cells, generating a hard standard with the loss of the glandular architecture.

Mentions: According to the Veterinary classification, from the nine examined tumors, three (33.3%) were classified as simple carcinoma (Fig. 3), and six (66.7%) as complex carcinoma (Fig. 4). But when it comes to the Human Medicine classification these nine cases were divided into: three (33.3%) simple carcinoma, being one tubulo-papilliferous, one ductal, one ductal infiltrative, and six (66.7%) metaplasic carcinomas. As for the histological malignity two tumors were degree I (22,2%) and seven tumors were degree III (77,8%). Receptors marking were positive for 5 animals, regardless of their mark intensity. From 9 analyzed female dogs, pyrethroids were detected in three animals distributed in the following manner: one (11.1%) female dog with 0.55 mg/g of deltamethrin and 0.32 mg/g of cyhalothrin; one (11.1%) female dog with 0.02 mg/g of deltamethrin and 0.05 mg/g of allethrin and one (11.1%) female dog with 0.03 mg/g of cypermethrin. Results are shown in table 1 and in the HPLC graphics (see Additional file 1).


Malignant mammary tumor in female dogs: environmental contaminants.

Andrade FH, Figueiroa FC, Bersano PR, Bissacot DZ, Rocha NS - Diagn Pathol (2010)

Simple Carcinoma. Neoplasia composed by proliferated epithelium cells, generating a hard standard with the loss of the glandular architecture.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2909155&req=5

Figure 3: Simple Carcinoma. Neoplasia composed by proliferated epithelium cells, generating a hard standard with the loss of the glandular architecture.
Mentions: According to the Veterinary classification, from the nine examined tumors, three (33.3%) were classified as simple carcinoma (Fig. 3), and six (66.7%) as complex carcinoma (Fig. 4). But when it comes to the Human Medicine classification these nine cases were divided into: three (33.3%) simple carcinoma, being one tubulo-papilliferous, one ductal, one ductal infiltrative, and six (66.7%) metaplasic carcinomas. As for the histological malignity two tumors were degree I (22,2%) and seven tumors were degree III (77,8%). Receptors marking were positive for 5 animals, regardless of their mark intensity. From 9 analyzed female dogs, pyrethroids were detected in three animals distributed in the following manner: one (11.1%) female dog with 0.55 mg/g of deltamethrin and 0.32 mg/g of cyhalothrin; one (11.1%) female dog with 0.02 mg/g of deltamethrin and 0.05 mg/g of allethrin and one (11.1%) female dog with 0.03 mg/g of cypermethrin. Results are shown in table 1 and in the HPLC graphics (see Additional file 1).

Bottom Line: Five tumors were positive for estrogen receptors in immunohistochemical analysis.The contamination level was observed in more aggressive tumors.Results suggest the possible involvement of pyrethroid in the canine mammary tumor carcinogenesis.

View Article: PubMed Central - HTML - PubMed

Affiliation: School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP) - Botucatu, SP, Brazil. rochanoeme@fmvz.unesp.br

ABSTRACT
Mammary tumors of female dogs have greatly increased in recent years, thus demanding rapid diagnosis and effective treatment in order to determine the animal survival. There is considerable scientific interest in the possible role of environmental contaminants in the etiology of mammary tumors, specifically in relation to synthetic chemical substances released into the environment to which living beings are either directly or indirectly exposed. In this study, the presence of pyrethroid insecticide was observed in adjacent adipose tissue of canine mammary tumor. High Precision Liquid Chromatography - HPLC was adapted to detect and identify environmental contaminants in adipose tissue adjacent to malignant mammary tumor in nine female dogs, without predilection for breed or age. After surgery, masses were carefully examined for malignant neoplastic lesions. Five grams of adipose tissue adjacent to the tumor were collected to detect of environmental contaminants. The identified pyrethroids were allethrin, cyhalothrin, cypermethrin, deltamethrin and tetramethrin, with a contamination level of 33.3%. Histopathology demonstrated six female dogs (66.7%) as having complex carcinoma and three (33.3%) with simple carcinoma. From these tumors, seven (77.8%) presented aggressiveness degree III and two (22.2%) degree I. Five tumors were positive for estrogen receptors in immunohistochemical analysis. The contamination level was observed in more aggressive tumors. This was the first report in which the level of environmental contaminants could be detected in adipose tissue of female dogs with malignant mammary tumor, by HPLC. Results suggest the possible involvement of pyrethroid in the canine mammary tumor carcinogenesis. Hence, the dog may be used as a sentinel animal for human breast cancer, since human beings share the same environment and basically have the same eating habits.

Show MeSH
Related in: MedlinePlus