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Snapshot Image Mapping Spectrometer (IMS) with high sampling density for hyperspectral microscopy.

Gao L, Kester RT, Hagen N, Tkaczyk TS - Opt Express (2010)

Bottom Line: The measured spectrum is from 450 nm to 650 nm and is sampled by 60 spectral channels with average sampling interval approximately 3.3 nm.The channel's spectral resolution is approximately 8nm.The spectral imaging results demonstrate the potential of the IMS for real-time cellular fluorescence imaging.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioengineering, Rice University, Houston, TX, 77005, USA. ttkaczyk@rice.edu

ABSTRACT
A snapshot Image Mapping Spectrometer (IMS) with high sampling density is developed for hyperspectral microscopy, measuring a datacube of dimensions 285 x 285 x 60 (x, y, lambda). The spatial resolution is approximately 0.45 microm with a FOV of 100 x 100 microm(2). The measured spectrum is from 450 nm to 650 nm and is sampled by 60 spectral channels with average sampling interval approximately 3.3 nm. The channel's spectral resolution is approximately 8nm. The spectral imaging results demonstrate the potential of the IMS for real-time cellular fluorescence imaging.

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Related in: MedlinePlus

(Media 2) 3D datacube 285 × 285 × 60 (x, y, λ) acquired by IMS. The displayed values result from assigning each voxel an opacity value in addition to its irradiance, and summing (with attenuation) all voxels along each ray defined by the lines of sight. The color bar is in arbitrary units proportional to photons/s/band.
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g012: (Media 2) 3D datacube 285 × 285 × 60 (x, y, λ) acquired by IMS. The displayed values result from assigning each voxel an opacity value in addition to its irradiance, and summing (with attenuation) all voxels along each ray defined by the lines of sight. The color bar is in arbitrary units proportional to photons/s/band.

Mentions: (Media 1) IMS spectral channel images. Cell Nuclei labeled with DAPI are visible in the blue spectral channels (460-472 nm); Filamentous actin labeled with Alexa Fluor 488 phalloidin are visible in the green spectral channels (520-549 nm); Mitochondria labeled with MitoTracker Red CMXRos are visible in the red spectral channels (596-649 nm).


Snapshot Image Mapping Spectrometer (IMS) with high sampling density for hyperspectral microscopy.

Gao L, Kester RT, Hagen N, Tkaczyk TS - Opt Express (2010)

(Media 2) 3D datacube 285 × 285 × 60 (x, y, λ) acquired by IMS. The displayed values result from assigning each voxel an opacity value in addition to its irradiance, and summing (with attenuation) all voxels along each ray defined by the lines of sight. The color bar is in arbitrary units proportional to photons/s/band.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2909105&req=5

g012: (Media 2) 3D datacube 285 × 285 × 60 (x, y, λ) acquired by IMS. The displayed values result from assigning each voxel an opacity value in addition to its irradiance, and summing (with attenuation) all voxels along each ray defined by the lines of sight. The color bar is in arbitrary units proportional to photons/s/band.
Mentions: (Media 1) IMS spectral channel images. Cell Nuclei labeled with DAPI are visible in the blue spectral channels (460-472 nm); Filamentous actin labeled with Alexa Fluor 488 phalloidin are visible in the green spectral channels (520-549 nm); Mitochondria labeled with MitoTracker Red CMXRos are visible in the red spectral channels (596-649 nm).

Bottom Line: The measured spectrum is from 450 nm to 650 nm and is sampled by 60 spectral channels with average sampling interval approximately 3.3 nm.The channel's spectral resolution is approximately 8nm.The spectral imaging results demonstrate the potential of the IMS for real-time cellular fluorescence imaging.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioengineering, Rice University, Houston, TX, 77005, USA. ttkaczyk@rice.edu

ABSTRACT
A snapshot Image Mapping Spectrometer (IMS) with high sampling density is developed for hyperspectral microscopy, measuring a datacube of dimensions 285 x 285 x 60 (x, y, lambda). The spatial resolution is approximately 0.45 microm with a FOV of 100 x 100 microm(2). The measured spectrum is from 450 nm to 650 nm and is sampled by 60 spectral channels with average sampling interval approximately 3.3 nm. The channel's spectral resolution is approximately 8nm. The spectral imaging results demonstrate the potential of the IMS for real-time cellular fluorescence imaging.

Show MeSH
Related in: MedlinePlus