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Snapshot Image Mapping Spectrometer (IMS) with high sampling density for hyperspectral microscopy.

Gao L, Kester RT, Hagen N, Tkaczyk TS - Opt Express (2010)

Bottom Line: The measured spectrum is from 450 nm to 650 nm and is sampled by 60 spectral channels with average sampling interval approximately 3.3 nm.The channel's spectral resolution is approximately 8nm.The spectral imaging results demonstrate the potential of the IMS for real-time cellular fluorescence imaging.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioengineering, Rice University, Houston, TX, 77005, USA. ttkaczyk@rice.edu

ABSTRACT
A snapshot Image Mapping Spectrometer (IMS) with high sampling density is developed for hyperspectral microscopy, measuring a datacube of dimensions 285 x 285 x 60 (x, y, lambda). The spatial resolution is approximately 0.45 microm with a FOV of 100 x 100 microm(2). The measured spectrum is from 450 nm to 650 nm and is sampled by 60 spectral channels with average sampling interval approximately 3.3 nm. The channel's spectral resolution is approximately 8nm. The spectral imaging results demonstrate the potential of the IMS for real-time cellular fluorescence imaging.

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BPAE cells; (a) direct imaging image and (b) IMS raw image. The 11-bit IMS raw image was captured with an integration time of 1 second.
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g010: BPAE cells; (a) direct imaging image and (b) IMS raw image. The 11-bit IMS raw image was captured with an integration time of 1 second.

Mentions: To test the spectral imaging capability of the high-sampling IMS in cellular fluorescence microscopy, triple-labeled BPAE cells (Invitrogen FluoCells prepared slide #1 (Cat. no. F14780)) were chosen as a sample. Mitochondria are labeled with MitoTracker Red CMXRos; filamentous actin is labeled with Alexa Fluor 488 phalloidin; and nuclei are labeled with DAPI. A triple-band filter set (Chroma 61001, DAPI/FITC/TRITC) was used to separate the excitation and emission light. The direct reference image was captured by a color camera at the microscope side-port and is shown in Fig. 10(a)Fig. 10


Snapshot Image Mapping Spectrometer (IMS) with high sampling density for hyperspectral microscopy.

Gao L, Kester RT, Hagen N, Tkaczyk TS - Opt Express (2010)

BPAE cells; (a) direct imaging image and (b) IMS raw image. The 11-bit IMS raw image was captured with an integration time of 1 second.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2909105&req=5

g010: BPAE cells; (a) direct imaging image and (b) IMS raw image. The 11-bit IMS raw image was captured with an integration time of 1 second.
Mentions: To test the spectral imaging capability of the high-sampling IMS in cellular fluorescence microscopy, triple-labeled BPAE cells (Invitrogen FluoCells prepared slide #1 (Cat. no. F14780)) were chosen as a sample. Mitochondria are labeled with MitoTracker Red CMXRos; filamentous actin is labeled with Alexa Fluor 488 phalloidin; and nuclei are labeled with DAPI. A triple-band filter set (Chroma 61001, DAPI/FITC/TRITC) was used to separate the excitation and emission light. The direct reference image was captured by a color camera at the microscope side-port and is shown in Fig. 10(a)Fig. 10

Bottom Line: The measured spectrum is from 450 nm to 650 nm and is sampled by 60 spectral channels with average sampling interval approximately 3.3 nm.The channel's spectral resolution is approximately 8nm.The spectral imaging results demonstrate the potential of the IMS for real-time cellular fluorescence imaging.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioengineering, Rice University, Houston, TX, 77005, USA. ttkaczyk@rice.edu

ABSTRACT
A snapshot Image Mapping Spectrometer (IMS) with high sampling density is developed for hyperspectral microscopy, measuring a datacube of dimensions 285 x 285 x 60 (x, y, lambda). The spatial resolution is approximately 0.45 microm with a FOV of 100 x 100 microm(2). The measured spectrum is from 450 nm to 650 nm and is sampled by 60 spectral channels with average sampling interval approximately 3.3 nm. The channel's spectral resolution is approximately 8nm. The spectral imaging results demonstrate the potential of the IMS for real-time cellular fluorescence imaging.

Show MeSH