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Snapshot Image Mapping Spectrometer (IMS) with high sampling density for hyperspectral microscopy.

Gao L, Kester RT, Hagen N, Tkaczyk TS - Opt Express (2010)

Bottom Line: The measured spectrum is from 450 nm to 650 nm and is sampled by 60 spectral channels with average sampling interval approximately 3.3 nm.The channel's spectral resolution is approximately 8nm.The spectral imaging results demonstrate the potential of the IMS for real-time cellular fluorescence imaging.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioengineering, Rice University, Houston, TX, 77005, USA. ttkaczyk@rice.edu

ABSTRACT
A snapshot Image Mapping Spectrometer (IMS) with high sampling density is developed for hyperspectral microscopy, measuring a datacube of dimensions 285 x 285 x 60 (x, y, lambda). The spatial resolution is approximately 0.45 microm with a FOV of 100 x 100 microm(2). The measured spectrum is from 450 nm to 650 nm and is sampled by 60 spectral channels with average sampling interval approximately 3.3 nm. The channel's spectral resolution is approximately 8nm. The spectral imaging results demonstrate the potential of the IMS for real-time cellular fluorescence imaging.

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“Elemental image” and assembly of full datacube
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g006: “Elemental image” and assembly of full datacube

Mentions: The raw data corresponding to an elemental object produces an “elemental image” (see Fig. 6Fig. 6


Snapshot Image Mapping Spectrometer (IMS) with high sampling density for hyperspectral microscopy.

Gao L, Kester RT, Hagen N, Tkaczyk TS - Opt Express (2010)

“Elemental image” and assembly of full datacube
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2909105&req=5

g006: “Elemental image” and assembly of full datacube
Mentions: The raw data corresponding to an elemental object produces an “elemental image” (see Fig. 6Fig. 6

Bottom Line: The measured spectrum is from 450 nm to 650 nm and is sampled by 60 spectral channels with average sampling interval approximately 3.3 nm.The channel's spectral resolution is approximately 8nm.The spectral imaging results demonstrate the potential of the IMS for real-time cellular fluorescence imaging.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioengineering, Rice University, Houston, TX, 77005, USA. ttkaczyk@rice.edu

ABSTRACT
A snapshot Image Mapping Spectrometer (IMS) with high sampling density is developed for hyperspectral microscopy, measuring a datacube of dimensions 285 x 285 x 60 (x, y, lambda). The spatial resolution is approximately 0.45 microm with a FOV of 100 x 100 microm(2). The measured spectrum is from 450 nm to 650 nm and is sampled by 60 spectral channels with average sampling interval approximately 3.3 nm. The channel's spectral resolution is approximately 8nm. The spectral imaging results demonstrate the potential of the IMS for real-time cellular fluorescence imaging.

Show MeSH