Limits...
Design and evaluation of an ultra-slim objective for in-vivo deep optical biopsy.

Landau SM, Liang C, Kester RT, Tkaczyk TS, Descour MR - Opt Express (2010)

Bottom Line: To ensure high-quality imaging performance, experimental tests were performed to characterize fiber bundle's light-coupling efficiency and simulations were performed to evaluate the impact of candidate lens materials' autofluorescence.A prototype of NA = 0.4, 250-microm field of view, ultra-slim objective optics was built and tested, yielding diffraction-limited performance and estimated resolution of 0.9 microm.When used in conjunction with a commercial coherent fiber bundle to relay the image formed by the objective, the measured resolution was 2.5 microm.

View Article: PubMed Central - PubMed

Affiliation: University of Arizona, College of Optical Sciences, 1630 E University Blvd, Tucson, AZ 85721, USA. slandau@optics.arizona.edu

ABSTRACT
An estimated 1.6 million breast biopsies are performed in the US each year. In order to provide real-time, in-vivo imaging with sub-cellular resolution for optical biopsies, we have designed an ultra-slim objective to fit inside the 1-mm-diameter hypodermic needles currently used for breast biopsies to image tissue stained by the fluorescent probe proflavine. To ensure high-quality imaging performance, experimental tests were performed to characterize fiber bundle's light-coupling efficiency and simulations were performed to evaluate the impact of candidate lens materials' autofluorescence. A prototype of NA = 0.4, 250-microm field of view, ultra-slim objective optics was built and tested, yielding diffraction-limited performance and estimated resolution of 0.9 microm. When used in conjunction with a commercial coherent fiber bundle to relay the image formed by the objective, the measured resolution was 2.5 microm.

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Grating with 25 μm period used for evaluating the quantitative performance of the objective. The annotated area represents the section over which the Strehl ratio was evaluated at each edge in the image.
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g012: Grating with 25 μm period used for evaluating the quantitative performance of the objective. The annotated area represents the section over which the Strehl ratio was evaluated at each edge in the image.

Mentions: In order to quantitatively test the image performance of the ultra-slim objective, an image of the grating shown in Fig. 12Fig. 12


Design and evaluation of an ultra-slim objective for in-vivo deep optical biopsy.

Landau SM, Liang C, Kester RT, Tkaczyk TS, Descour MR - Opt Express (2010)

Grating with 25 μm period used for evaluating the quantitative performance of the objective. The annotated area represents the section over which the Strehl ratio was evaluated at each edge in the image.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2909099&req=5

g012: Grating with 25 μm period used for evaluating the quantitative performance of the objective. The annotated area represents the section over which the Strehl ratio was evaluated at each edge in the image.
Mentions: In order to quantitatively test the image performance of the ultra-slim objective, an image of the grating shown in Fig. 12Fig. 12

Bottom Line: To ensure high-quality imaging performance, experimental tests were performed to characterize fiber bundle's light-coupling efficiency and simulations were performed to evaluate the impact of candidate lens materials' autofluorescence.A prototype of NA = 0.4, 250-microm field of view, ultra-slim objective optics was built and tested, yielding diffraction-limited performance and estimated resolution of 0.9 microm.When used in conjunction with a commercial coherent fiber bundle to relay the image formed by the objective, the measured resolution was 2.5 microm.

View Article: PubMed Central - PubMed

Affiliation: University of Arizona, College of Optical Sciences, 1630 E University Blvd, Tucson, AZ 85721, USA. slandau@optics.arizona.edu

ABSTRACT
An estimated 1.6 million breast biopsies are performed in the US each year. In order to provide real-time, in-vivo imaging with sub-cellular resolution for optical biopsies, we have designed an ultra-slim objective to fit inside the 1-mm-diameter hypodermic needles currently used for breast biopsies to image tissue stained by the fluorescent probe proflavine. To ensure high-quality imaging performance, experimental tests were performed to characterize fiber bundle's light-coupling efficiency and simulations were performed to evaluate the impact of candidate lens materials' autofluorescence. A prototype of NA = 0.4, 250-microm field of view, ultra-slim objective optics was built and tested, yielding diffraction-limited performance and estimated resolution of 0.9 microm. When used in conjunction with a commercial coherent fiber bundle to relay the image formed by the objective, the measured resolution was 2.5 microm.

Show MeSH