Limits...
Design and evaluation of an ultra-slim objective for in-vivo deep optical biopsy.

Landau SM, Liang C, Kester RT, Tkaczyk TS, Descour MR - Opt Express (2010)

Bottom Line: To ensure high-quality imaging performance, experimental tests were performed to characterize fiber bundle's light-coupling efficiency and simulations were performed to evaluate the impact of candidate lens materials' autofluorescence.A prototype of NA = 0.4, 250-microm field of view, ultra-slim objective optics was built and tested, yielding diffraction-limited performance and estimated resolution of 0.9 microm.When used in conjunction with a commercial coherent fiber bundle to relay the image formed by the objective, the measured resolution was 2.5 microm.

View Article: PubMed Central - PubMed

Affiliation: University of Arizona, College of Optical Sciences, 1630 E University Blvd, Tucson, AZ 85721, USA. slandau@optics.arizona.edu

ABSTRACT
An estimated 1.6 million breast biopsies are performed in the US each year. In order to provide real-time, in-vivo imaging with sub-cellular resolution for optical biopsies, we have designed an ultra-slim objective to fit inside the 1-mm-diameter hypodermic needles currently used for breast biopsies to image tissue stained by the fluorescent probe proflavine. To ensure high-quality imaging performance, experimental tests were performed to characterize fiber bundle's light-coupling efficiency and simulations were performed to evaluate the impact of candidate lens materials' autofluorescence. A prototype of NA = 0.4, 250-microm field of view, ultra-slim objective optics was built and tested, yielding diffraction-limited performance and estimated resolution of 0.9 microm. When used in conjunction with a commercial coherent fiber bundle to relay the image formed by the objective, the measured resolution was 2.5 microm.

Show MeSH
Experimental setup for measurement of light-coupling efficiency into a coherent fiber bundle as a function of principal ray angle of incidence.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2909099&req=5

g007: Experimental setup for measurement of light-coupling efficiency into a coherent fiber bundle as a function of principal ray angle of incidence.

Mentions: Because the light level for fluorescent imaging is typically low, an experimental determination of the light-coupling efficiency with respect to increasing principal ray angle, and thus an important design constraint, was critical. The experimental setup was designed to simulate the ultra-slim objective coupling efficiency by using the same coherent fiber bundle. For further consistency, the NA of the objective focusing the light onto the fiber bundle is the same as the image-space NA of the ultra-slim objective. Although this configuration was specific to our design, the experimental setup could be readily modified to test other fiber bundles, wavelengths, or NAs. The setup used [see Fig. 7Fig. 7


Design and evaluation of an ultra-slim objective for in-vivo deep optical biopsy.

Landau SM, Liang C, Kester RT, Tkaczyk TS, Descour MR - Opt Express (2010)

Experimental setup for measurement of light-coupling efficiency into a coherent fiber bundle as a function of principal ray angle of incidence.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2909099&req=5

g007: Experimental setup for measurement of light-coupling efficiency into a coherent fiber bundle as a function of principal ray angle of incidence.
Mentions: Because the light level for fluorescent imaging is typically low, an experimental determination of the light-coupling efficiency with respect to increasing principal ray angle, and thus an important design constraint, was critical. The experimental setup was designed to simulate the ultra-slim objective coupling efficiency by using the same coherent fiber bundle. For further consistency, the NA of the objective focusing the light onto the fiber bundle is the same as the image-space NA of the ultra-slim objective. Although this configuration was specific to our design, the experimental setup could be readily modified to test other fiber bundles, wavelengths, or NAs. The setup used [see Fig. 7Fig. 7

Bottom Line: To ensure high-quality imaging performance, experimental tests were performed to characterize fiber bundle's light-coupling efficiency and simulations were performed to evaluate the impact of candidate lens materials' autofluorescence.A prototype of NA = 0.4, 250-microm field of view, ultra-slim objective optics was built and tested, yielding diffraction-limited performance and estimated resolution of 0.9 microm.When used in conjunction with a commercial coherent fiber bundle to relay the image formed by the objective, the measured resolution was 2.5 microm.

View Article: PubMed Central - PubMed

Affiliation: University of Arizona, College of Optical Sciences, 1630 E University Blvd, Tucson, AZ 85721, USA. slandau@optics.arizona.edu

ABSTRACT
An estimated 1.6 million breast biopsies are performed in the US each year. In order to provide real-time, in-vivo imaging with sub-cellular resolution for optical biopsies, we have designed an ultra-slim objective to fit inside the 1-mm-diameter hypodermic needles currently used for breast biopsies to image tissue stained by the fluorescent probe proflavine. To ensure high-quality imaging performance, experimental tests were performed to characterize fiber bundle's light-coupling efficiency and simulations were performed to evaluate the impact of candidate lens materials' autofluorescence. A prototype of NA = 0.4, 250-microm field of view, ultra-slim objective optics was built and tested, yielding diffraction-limited performance and estimated resolution of 0.9 microm. When used in conjunction with a commercial coherent fiber bundle to relay the image formed by the objective, the measured resolution was 2.5 microm.

Show MeSH