Limits...
Design and evaluation of an ultra-slim objective for in-vivo deep optical biopsy.

Landau SM, Liang C, Kester RT, Tkaczyk TS, Descour MR - Opt Express (2010)

Bottom Line: To ensure high-quality imaging performance, experimental tests were performed to characterize fiber bundle's light-coupling efficiency and simulations were performed to evaluate the impact of candidate lens materials' autofluorescence.A prototype of NA = 0.4, 250-microm field of view, ultra-slim objective optics was built and tested, yielding diffraction-limited performance and estimated resolution of 0.9 microm.When used in conjunction with a commercial coherent fiber bundle to relay the image formed by the objective, the measured resolution was 2.5 microm.

View Article: PubMed Central - PubMed

Affiliation: University of Arizona, College of Optical Sciences, 1630 E University Blvd, Tucson, AZ 85721, USA. slandau@optics.arizona.edu

ABSTRACT
An estimated 1.6 million breast biopsies are performed in the US each year. In order to provide real-time, in-vivo imaging with sub-cellular resolution for optical biopsies, we have designed an ultra-slim objective to fit inside the 1-mm-diameter hypodermic needles currently used for breast biopsies to image tissue stained by the fluorescent probe proflavine. To ensure high-quality imaging performance, experimental tests were performed to characterize fiber bundle's light-coupling efficiency and simulations were performed to evaluate the impact of candidate lens materials' autofluorescence. A prototype of NA = 0.4, 250-microm field of view, ultra-slim objective optics was built and tested, yielding diffraction-limited performance and estimated resolution of 0.9 microm. When used in conjunction with a commercial coherent fiber bundle to relay the image formed by the objective, the measured resolution was 2.5 microm.

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Diagrams illustrating θt and its effect on coupling. TEM(0,0) and TEM(0,1) stand for transverse electromagnetic wave zeroth order and first order mode, respectively. (a) Illustration of a ray bundle intersected by the transition angle, (b) Diagram of fiber emission depending on the mode of light.
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g005: Diagrams illustrating θt and its effect on coupling. TEM(0,0) and TEM(0,1) stand for transverse electromagnetic wave zeroth order and first order mode, respectively. (a) Illustration of a ray bundle intersected by the transition angle, (b) Diagram of fiber emission depending on the mode of light.

Mentions: Second, for a given fiber there exists a transition angle, θt, dependent on the wavelength, fiber core diameter, and fiber NA [22]. Rays entering the fiber below θt excite the zeroth order mode, and rays with an incident angle greater than θt excite first order mode. For a ray bundle that straddles θt [see Fig. 5(a)Fig. 5


Design and evaluation of an ultra-slim objective for in-vivo deep optical biopsy.

Landau SM, Liang C, Kester RT, Tkaczyk TS, Descour MR - Opt Express (2010)

Diagrams illustrating θt and its effect on coupling. TEM(0,0) and TEM(0,1) stand for transverse electromagnetic wave zeroth order and first order mode, respectively. (a) Illustration of a ray bundle intersected by the transition angle, (b) Diagram of fiber emission depending on the mode of light.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2909099&req=5

g005: Diagrams illustrating θt and its effect on coupling. TEM(0,0) and TEM(0,1) stand for transverse electromagnetic wave zeroth order and first order mode, respectively. (a) Illustration of a ray bundle intersected by the transition angle, (b) Diagram of fiber emission depending on the mode of light.
Mentions: Second, for a given fiber there exists a transition angle, θt, dependent on the wavelength, fiber core diameter, and fiber NA [22]. Rays entering the fiber below θt excite the zeroth order mode, and rays with an incident angle greater than θt excite first order mode. For a ray bundle that straddles θt [see Fig. 5(a)Fig. 5

Bottom Line: To ensure high-quality imaging performance, experimental tests were performed to characterize fiber bundle's light-coupling efficiency and simulations were performed to evaluate the impact of candidate lens materials' autofluorescence.A prototype of NA = 0.4, 250-microm field of view, ultra-slim objective optics was built and tested, yielding diffraction-limited performance and estimated resolution of 0.9 microm.When used in conjunction with a commercial coherent fiber bundle to relay the image formed by the objective, the measured resolution was 2.5 microm.

View Article: PubMed Central - PubMed

Affiliation: University of Arizona, College of Optical Sciences, 1630 E University Blvd, Tucson, AZ 85721, USA. slandau@optics.arizona.edu

ABSTRACT
An estimated 1.6 million breast biopsies are performed in the US each year. In order to provide real-time, in-vivo imaging with sub-cellular resolution for optical biopsies, we have designed an ultra-slim objective to fit inside the 1-mm-diameter hypodermic needles currently used for breast biopsies to image tissue stained by the fluorescent probe proflavine. To ensure high-quality imaging performance, experimental tests were performed to characterize fiber bundle's light-coupling efficiency and simulations were performed to evaluate the impact of candidate lens materials' autofluorescence. A prototype of NA = 0.4, 250-microm field of view, ultra-slim objective optics was built and tested, yielding diffraction-limited performance and estimated resolution of 0.9 microm. When used in conjunction with a commercial coherent fiber bundle to relay the image formed by the objective, the measured resolution was 2.5 microm.

Show MeSH