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High speed optical coherence microscopy with autofocus adjustment and a miniaturized endoscopic imaging probe.

Aguirre AD, Sawinski J, Huang SW, Zhou C, Denk W, Fujimoto JG - Opt Express (2010)

Bottom Line: The system utilized a novel polarization compensation method to combat wavelength dependent source polarization and achieve broadband electro-optic phase modulation compatible with ultrahigh axial resolution.In addition, the system incorporated an auto-focusing feature that enables precise, near real-time alignment of the confocal and coherence gates in tissue, allowing user-friendly optimization of image quality during the imaging procedure.Ex vivo cellular images of human esophagus, colon, and cervix as well as in vivo results from human skin are presented.

View Article: PubMed Central - PubMed

Affiliation: Research Laboratory of Electronics and Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 USA.

ABSTRACT
Optical coherence microscopy (OCM) is a promising technique for high resolution cellular imaging in human tissues. An OCM system for high-speed en face cellular resolution imaging was developed at 1060 nm wavelength at frame rates up to 5 Hz with resolutions of < 4 microm axial and < 2 microm transverse. The system utilized a novel polarization compensation method to combat wavelength dependent source polarization and achieve broadband electro-optic phase modulation compatible with ultrahigh axial resolution. In addition, the system incorporated an auto-focusing feature that enables precise, near real-time alignment of the confocal and coherence gates in tissue, allowing user-friendly optimization of image quality during the imaging procedure. Ex vivo cellular images of human esophagus, colon, and cervix as well as in vivo results from human skin are presented. Finally, the system design is demonstrated with a miniaturized piezoelectric fiber-scanning probe which can be adapted for laparoscopic and endoscopic imaging applications.

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Endoscopic OCM imaging. (a) USAF target demonstrating scan field of view and lateral resolution <2 µm. (b,c) Ex vivo images of human colon acquired at 2 frames per second. Goblet cells, gc. (d) In vivo image of human skin acquired at 4 frames per second. Epidermal cells, ec. Scale bar, 50 µm.
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g011: Endoscopic OCM imaging. (a) USAF target demonstrating scan field of view and lateral resolution <2 µm. (b,c) Ex vivo images of human colon acquired at 2 frames per second. Goblet cells, gc. (d) In vivo image of human skin acquired at 4 frames per second. Epidermal cells, ec. Scale bar, 50 µm.

Mentions: Figure 11Fig. 11


High speed optical coherence microscopy with autofocus adjustment and a miniaturized endoscopic imaging probe.

Aguirre AD, Sawinski J, Huang SW, Zhou C, Denk W, Fujimoto JG - Opt Express (2010)

Endoscopic OCM imaging. (a) USAF target demonstrating scan field of view and lateral resolution <2 µm. (b,c) Ex vivo images of human colon acquired at 2 frames per second. Goblet cells, gc. (d) In vivo image of human skin acquired at 4 frames per second. Epidermal cells, ec. Scale bar, 50 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2908909&req=5

g011: Endoscopic OCM imaging. (a) USAF target demonstrating scan field of view and lateral resolution <2 µm. (b,c) Ex vivo images of human colon acquired at 2 frames per second. Goblet cells, gc. (d) In vivo image of human skin acquired at 4 frames per second. Epidermal cells, ec. Scale bar, 50 µm.
Mentions: Figure 11Fig. 11

Bottom Line: The system utilized a novel polarization compensation method to combat wavelength dependent source polarization and achieve broadband electro-optic phase modulation compatible with ultrahigh axial resolution.In addition, the system incorporated an auto-focusing feature that enables precise, near real-time alignment of the confocal and coherence gates in tissue, allowing user-friendly optimization of image quality during the imaging procedure.Ex vivo cellular images of human esophagus, colon, and cervix as well as in vivo results from human skin are presented.

View Article: PubMed Central - PubMed

Affiliation: Research Laboratory of Electronics and Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 USA.

ABSTRACT
Optical coherence microscopy (OCM) is a promising technique for high resolution cellular imaging in human tissues. An OCM system for high-speed en face cellular resolution imaging was developed at 1060 nm wavelength at frame rates up to 5 Hz with resolutions of < 4 microm axial and < 2 microm transverse. The system utilized a novel polarization compensation method to combat wavelength dependent source polarization and achieve broadband electro-optic phase modulation compatible with ultrahigh axial resolution. In addition, the system incorporated an auto-focusing feature that enables precise, near real-time alignment of the confocal and coherence gates in tissue, allowing user-friendly optimization of image quality during the imaging procedure. Ex vivo cellular images of human esophagus, colon, and cervix as well as in vivo results from human skin are presented. Finally, the system design is demonstrated with a miniaturized piezoelectric fiber-scanning probe which can be adapted for laparoscopic and endoscopic imaging applications.

Show MeSH