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High speed optical coherence microscopy with autofocus adjustment and a miniaturized endoscopic imaging probe.

Aguirre AD, Sawinski J, Huang SW, Zhou C, Denk W, Fujimoto JG - Opt Express (2010)

Bottom Line: The system utilized a novel polarization compensation method to combat wavelength dependent source polarization and achieve broadband electro-optic phase modulation compatible with ultrahigh axial resolution.In addition, the system incorporated an auto-focusing feature that enables precise, near real-time alignment of the confocal and coherence gates in tissue, allowing user-friendly optimization of image quality during the imaging procedure.Ex vivo cellular images of human esophagus, colon, and cervix as well as in vivo results from human skin are presented.

View Article: PubMed Central - PubMed

Affiliation: Research Laboratory of Electronics and Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 USA.

ABSTRACT
Optical coherence microscopy (OCM) is a promising technique for high resolution cellular imaging in human tissues. An OCM system for high-speed en face cellular resolution imaging was developed at 1060 nm wavelength at frame rates up to 5 Hz with resolutions of < 4 microm axial and < 2 microm transverse. The system utilized a novel polarization compensation method to combat wavelength dependent source polarization and achieve broadband electro-optic phase modulation compatible with ultrahigh axial resolution. In addition, the system incorporated an auto-focusing feature that enables precise, near real-time alignment of the confocal and coherence gates in tissue, allowing user-friendly optimization of image quality during the imaging procedure. Ex vivo cellular images of human esophagus, colon, and cervix as well as in vivo results from human skin are presented. Finally, the system design is demonstrated with a miniaturized piezoelectric fiber-scanning probe which can be adapted for laparoscopic and endoscopic imaging applications.

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Algorithm for fast autofocusing in scattering tissues.
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g008: Algorithm for fast autofocusing in scattering tissues.

Mentions: An autofocusing algorithm was developed using lateral-priority, cross-sectional images to locate the confocal focus. The algorithm was implemented in the OCM imaging software and enabled rapid image optimization during real-time display. Figure 8Fig. 8


High speed optical coherence microscopy with autofocus adjustment and a miniaturized endoscopic imaging probe.

Aguirre AD, Sawinski J, Huang SW, Zhou C, Denk W, Fujimoto JG - Opt Express (2010)

Algorithm for fast autofocusing in scattering tissues.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2908909&req=5

g008: Algorithm for fast autofocusing in scattering tissues.
Mentions: An autofocusing algorithm was developed using lateral-priority, cross-sectional images to locate the confocal focus. The algorithm was implemented in the OCM imaging software and enabled rapid image optimization during real-time display. Figure 8Fig. 8

Bottom Line: The system utilized a novel polarization compensation method to combat wavelength dependent source polarization and achieve broadband electro-optic phase modulation compatible with ultrahigh axial resolution.In addition, the system incorporated an auto-focusing feature that enables precise, near real-time alignment of the confocal and coherence gates in tissue, allowing user-friendly optimization of image quality during the imaging procedure.Ex vivo cellular images of human esophagus, colon, and cervix as well as in vivo results from human skin are presented.

View Article: PubMed Central - PubMed

Affiliation: Research Laboratory of Electronics and Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 USA.

ABSTRACT
Optical coherence microscopy (OCM) is a promising technique for high resolution cellular imaging in human tissues. An OCM system for high-speed en face cellular resolution imaging was developed at 1060 nm wavelength at frame rates up to 5 Hz with resolutions of < 4 microm axial and < 2 microm transverse. The system utilized a novel polarization compensation method to combat wavelength dependent source polarization and achieve broadband electro-optic phase modulation compatible with ultrahigh axial resolution. In addition, the system incorporated an auto-focusing feature that enables precise, near real-time alignment of the confocal and coherence gates in tissue, allowing user-friendly optimization of image quality during the imaging procedure. Ex vivo cellular images of human esophagus, colon, and cervix as well as in vivo results from human skin are presented. Finally, the system design is demonstrated with a miniaturized piezoelectric fiber-scanning probe which can be adapted for laparoscopic and endoscopic imaging applications.

Show MeSH