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High speed optical coherence microscopy with autofocus adjustment and a miniaturized endoscopic imaging probe.

Aguirre AD, Sawinski J, Huang SW, Zhou C, Denk W, Fujimoto JG - Opt Express (2010)

Bottom Line: The system utilized a novel polarization compensation method to combat wavelength dependent source polarization and achieve broadband electro-optic phase modulation compatible with ultrahigh axial resolution.In addition, the system incorporated an auto-focusing feature that enables precise, near real-time alignment of the confocal and coherence gates in tissue, allowing user-friendly optimization of image quality during the imaging procedure.Ex vivo cellular images of human esophagus, colon, and cervix as well as in vivo results from human skin are presented.

View Article: PubMed Central - PubMed

Affiliation: Research Laboratory of Electronics and Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 USA.

ABSTRACT
Optical coherence microscopy (OCM) is a promising technique for high resolution cellular imaging in human tissues. An OCM system for high-speed en face cellular resolution imaging was developed at 1060 nm wavelength at frame rates up to 5 Hz with resolutions of < 4 microm axial and < 2 microm transverse. The system utilized a novel polarization compensation method to combat wavelength dependent source polarization and achieve broadband electro-optic phase modulation compatible with ultrahigh axial resolution. In addition, the system incorporated an auto-focusing feature that enables precise, near real-time alignment of the confocal and coherence gates in tissue, allowing user-friendly optimization of image quality during the imaging procedure. Ex vivo cellular images of human esophagus, colon, and cervix as well as in vivo results from human skin are presented. Finally, the system design is demonstrated with a miniaturized piezoelectric fiber-scanning probe which can be adapted for laparoscopic and endoscopic imaging applications.

Show MeSH
Dispersion-balanced axial coherence point spread function achieved with polarization management. The axial resolution (a) measured 4.3 µm in air, corresponding to 3.1 µm in tissue. The Fourier transform of the point spread function (b), measures ~137 nm in spectral full-width at half maximum
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g003: Dispersion-balanced axial coherence point spread function achieved with polarization management. The axial resolution (a) measured 4.3 µm in air, corresponding to 3.1 µm in tissue. The Fourier transform of the point spread function (b), measures ~137 nm in spectral full-width at half maximum

Mentions: Using this scheme in combination with the dispersion management techniques described above, the full optical bandwidth of the light source could be supported by the EOM. Figure 3(a)Fig. 3


High speed optical coherence microscopy with autofocus adjustment and a miniaturized endoscopic imaging probe.

Aguirre AD, Sawinski J, Huang SW, Zhou C, Denk W, Fujimoto JG - Opt Express (2010)

Dispersion-balanced axial coherence point spread function achieved with polarization management. The axial resolution (a) measured 4.3 µm in air, corresponding to 3.1 µm in tissue. The Fourier transform of the point spread function (b), measures ~137 nm in spectral full-width at half maximum
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2908909&req=5

g003: Dispersion-balanced axial coherence point spread function achieved with polarization management. The axial resolution (a) measured 4.3 µm in air, corresponding to 3.1 µm in tissue. The Fourier transform of the point spread function (b), measures ~137 nm in spectral full-width at half maximum
Mentions: Using this scheme in combination with the dispersion management techniques described above, the full optical bandwidth of the light source could be supported by the EOM. Figure 3(a)Fig. 3

Bottom Line: The system utilized a novel polarization compensation method to combat wavelength dependent source polarization and achieve broadband electro-optic phase modulation compatible with ultrahigh axial resolution.In addition, the system incorporated an auto-focusing feature that enables precise, near real-time alignment of the confocal and coherence gates in tissue, allowing user-friendly optimization of image quality during the imaging procedure.Ex vivo cellular images of human esophagus, colon, and cervix as well as in vivo results from human skin are presented.

View Article: PubMed Central - PubMed

Affiliation: Research Laboratory of Electronics and Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 USA.

ABSTRACT
Optical coherence microscopy (OCM) is a promising technique for high resolution cellular imaging in human tissues. An OCM system for high-speed en face cellular resolution imaging was developed at 1060 nm wavelength at frame rates up to 5 Hz with resolutions of < 4 microm axial and < 2 microm transverse. The system utilized a novel polarization compensation method to combat wavelength dependent source polarization and achieve broadband electro-optic phase modulation compatible with ultrahigh axial resolution. In addition, the system incorporated an auto-focusing feature that enables precise, near real-time alignment of the confocal and coherence gates in tissue, allowing user-friendly optimization of image quality during the imaging procedure. Ex vivo cellular images of human esophagus, colon, and cervix as well as in vivo results from human skin are presented. Finally, the system design is demonstrated with a miniaturized piezoelectric fiber-scanning probe which can be adapted for laparoscopic and endoscopic imaging applications.

Show MeSH