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High speed optical coherence microscopy with autofocus adjustment and a miniaturized endoscopic imaging probe.

Aguirre AD, Sawinski J, Huang SW, Zhou C, Denk W, Fujimoto JG - Opt Express (2010)

Bottom Line: The system utilized a novel polarization compensation method to combat wavelength dependent source polarization and achieve broadband electro-optic phase modulation compatible with ultrahigh axial resolution.In addition, the system incorporated an auto-focusing feature that enables precise, near real-time alignment of the confocal and coherence gates in tissue, allowing user-friendly optimization of image quality during the imaging procedure.Ex vivo cellular images of human esophagus, colon, and cervix as well as in vivo results from human skin are presented.

View Article: PubMed Central - PubMed

Affiliation: Research Laboratory of Electronics and Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 USA.

ABSTRACT
Optical coherence microscopy (OCM) is a promising technique for high resolution cellular imaging in human tissues. An OCM system for high-speed en face cellular resolution imaging was developed at 1060 nm wavelength at frame rates up to 5 Hz with resolutions of < 4 microm axial and < 2 microm transverse. The system utilized a novel polarization compensation method to combat wavelength dependent source polarization and achieve broadband electro-optic phase modulation compatible with ultrahigh axial resolution. In addition, the system incorporated an auto-focusing feature that enables precise, near real-time alignment of the confocal and coherence gates in tissue, allowing user-friendly optimization of image quality during the imaging procedure. Ex vivo cellular images of human esophagus, colon, and cervix as well as in vivo results from human skin are presented. Finally, the system design is demonstrated with a miniaturized piezoelectric fiber-scanning probe which can be adapted for laparoscopic and endoscopic imaging applications.

Show MeSH
High-speed OCM imaging system. The system operates at 1060 nm center wavelength using a broadband electro-optic waveguide phase modulator. TIA, transimpedance amplifier. BPF, bandpass filter. PD, photodiode. VGA, variable-gain amplifier. A/D, analog-to-digital converter. PC, personal computer. D/A, digital-to-analog converter. PM, polarization-maintaining. EOM, electro-optic modulator.
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g001: High-speed OCM imaging system. The system operates at 1060 nm center wavelength using a broadband electro-optic waveguide phase modulator. TIA, transimpedance amplifier. BPF, bandpass filter. PD, photodiode. VGA, variable-gain amplifier. A/D, analog-to-digital converter. PC, personal computer. D/A, digital-to-analog converter. PM, polarization-maintaining. EOM, electro-optic modulator.

Mentions: Figure 1Fig. 1


High speed optical coherence microscopy with autofocus adjustment and a miniaturized endoscopic imaging probe.

Aguirre AD, Sawinski J, Huang SW, Zhou C, Denk W, Fujimoto JG - Opt Express (2010)

High-speed OCM imaging system. The system operates at 1060 nm center wavelength using a broadband electro-optic waveguide phase modulator. TIA, transimpedance amplifier. BPF, bandpass filter. PD, photodiode. VGA, variable-gain amplifier. A/D, analog-to-digital converter. PC, personal computer. D/A, digital-to-analog converter. PM, polarization-maintaining. EOM, electro-optic modulator.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2908909&req=5

g001: High-speed OCM imaging system. The system operates at 1060 nm center wavelength using a broadband electro-optic waveguide phase modulator. TIA, transimpedance amplifier. BPF, bandpass filter. PD, photodiode. VGA, variable-gain amplifier. A/D, analog-to-digital converter. PC, personal computer. D/A, digital-to-analog converter. PM, polarization-maintaining. EOM, electro-optic modulator.
Mentions: Figure 1Fig. 1

Bottom Line: The system utilized a novel polarization compensation method to combat wavelength dependent source polarization and achieve broadband electro-optic phase modulation compatible with ultrahigh axial resolution.In addition, the system incorporated an auto-focusing feature that enables precise, near real-time alignment of the confocal and coherence gates in tissue, allowing user-friendly optimization of image quality during the imaging procedure.Ex vivo cellular images of human esophagus, colon, and cervix as well as in vivo results from human skin are presented.

View Article: PubMed Central - PubMed

Affiliation: Research Laboratory of Electronics and Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 USA.

ABSTRACT
Optical coherence microscopy (OCM) is a promising technique for high resolution cellular imaging in human tissues. An OCM system for high-speed en face cellular resolution imaging was developed at 1060 nm wavelength at frame rates up to 5 Hz with resolutions of < 4 microm axial and < 2 microm transverse. The system utilized a novel polarization compensation method to combat wavelength dependent source polarization and achieve broadband electro-optic phase modulation compatible with ultrahigh axial resolution. In addition, the system incorporated an auto-focusing feature that enables precise, near real-time alignment of the confocal and coherence gates in tissue, allowing user-friendly optimization of image quality during the imaging procedure. Ex vivo cellular images of human esophagus, colon, and cervix as well as in vivo results from human skin are presented. Finally, the system design is demonstrated with a miniaturized piezoelectric fiber-scanning probe which can be adapted for laparoscopic and endoscopic imaging applications.

Show MeSH