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Acute stress and chronic stress change brain-derived neurotrophic factor (BDNF) and tyrosine kinase-coupled receptor (TrkB) expression in both young and aged rat hippocampus.

Shi SS, Shao SH, Yuan BP, Pan F, Li ZL - Yonsei Med. J. (2010)

Bottom Line: The short AS induced a significant increase in BDNF mRNA and protein in both age groups, but the changes in the young group were substantially greater than those of the aged group (p < 0.005).The CMRS resulted in a decrease in BDNF mRNA and protein, but a significant increase in TrkB mRNA in both young and age groups.The results indicated that the up/down-regulation of BDNF and TrkB were affected by aging and the stimulus paradigm, which might reflect important mechanisms by which the hippocampus copes with stressful stimuli.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Psychology, Institute of Biochemistry and Molecular Biology, Binzhou Medical University, Yantai, Shandong, China.

ABSTRACT

Purpose: The purpose of this study is to explore the dynamic change of brainderived neurotrophic factor (BDNF) mRNA, protein, and tyrosine kinase-coupled receptor (TrkB) mRNA of the rat hippocampus under different stress conditions and to explore the influence of senescence on the productions expression.

Materials and methods: By using forced-swimming in 4 degrees C cold ice water and 25 degrees C warm water, young and aged male rats were randomly divided into acute stress (AS) and chronic mild repeated stress (CMRS) subgroups, respectively. BDNF productions and TrkB mRNA in the hippocampus were detected by using Western-blotting and reverse transcription-polymerase chain reaction (RT-PCR), separately, at 15, 30, 60, 180, and 720 min after the last stress session.

Results: The short AS induced a significant increase in BDNF mRNA and protein in both age groups, but the changes in the young group were substantially greater than those of the aged group (p < 0.005). The CMRS resulted in a decrease in BDNF mRNA and protein, but a significant increase in TrkB mRNA in both young and age groups. The expression of BDNF mRNA and protein in the AS groups were higher than in the CMRS groups at 15, 30, and 60 min after stress.

Conclusion: The results indicated that the up/down-regulation of BDNF and TrkB were affected by aging and the stimulus paradigm, which might reflect important mechanisms by which the hippocampus copes with stressful stimuli.

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Related in: MedlinePlus

Time course of BDNF protein expression in the hippocampus measured by Western blotting after chronic repeated stress. (A) Representative photographic film, illustrating the dynamic changes in BDNF protein from the young and aged CMRS groups. (B) Results of statistical analysis of BDNF protein expression in control groups (unstressed, 0 min) and stressed animals after 15, 30, 60, 180, 720 min of stress (in two stress conditions). The results were expressed as mean ± SEM. *p < 0.05 and **p < 0.001 vs. young control group. †p < 0.05 and ‡p < 0.001 vs. aged control group. n = 5-6 rats per each time point studied in two independent stress conditions. BDNF, brain-derived neurotrophic factor; CMRS, chronic mild repeated stress.
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Figure 7: Time course of BDNF protein expression in the hippocampus measured by Western blotting after chronic repeated stress. (A) Representative photographic film, illustrating the dynamic changes in BDNF protein from the young and aged CMRS groups. (B) Results of statistical analysis of BDNF protein expression in control groups (unstressed, 0 min) and stressed animals after 15, 30, 60, 180, 720 min of stress (in two stress conditions). The results were expressed as mean ± SEM. *p < 0.05 and **p < 0.001 vs. young control group. †p < 0.05 and ‡p < 0.001 vs. aged control group. n = 5-6 rats per each time point studied in two independent stress conditions. BDNF, brain-derived neurotrophic factor; CMRS, chronic mild repeated stress.

Mentions: Western blotting was performed in the hippocampus to examine discrete modifications of the BDNF protein on control groups (0 min) and stress-exposed groups at 15, 30, 60, 180, and 720 min after stress. After AS, we observed a rapid increase in BDNF at 15 min (as well as 30 and 60 min) throughout the entire hippocampus, in both young and aged groups. A decrease occurred at 180 min and the levels had approached the original condition by 720 min. The maximum expression of BDNF in both young and aged AS groups were observed at 30 min (Fig. 6). After chronic repeated stress, the expression of BDNF showed a dynamic change at 15, 30, 60, 180, and 720 min, whereas the levels were lower than those measured in both the young and aged control groups. However, we observed that the BDNF protein level detected in the young group, 15 min after the last repeated stimulus, was higher than that measured at all other time points, and displayed a remarkable change from 15 min to 180 min. In contrast, the expression level in the aged group was lower than that of the young group at several time points and showed a similarly low level from 60 min to 720 min (Fig. 7).


Acute stress and chronic stress change brain-derived neurotrophic factor (BDNF) and tyrosine kinase-coupled receptor (TrkB) expression in both young and aged rat hippocampus.

Shi SS, Shao SH, Yuan BP, Pan F, Li ZL - Yonsei Med. J. (2010)

Time course of BDNF protein expression in the hippocampus measured by Western blotting after chronic repeated stress. (A) Representative photographic film, illustrating the dynamic changes in BDNF protein from the young and aged CMRS groups. (B) Results of statistical analysis of BDNF protein expression in control groups (unstressed, 0 min) and stressed animals after 15, 30, 60, 180, 720 min of stress (in two stress conditions). The results were expressed as mean ± SEM. *p < 0.05 and **p < 0.001 vs. young control group. †p < 0.05 and ‡p < 0.001 vs. aged control group. n = 5-6 rats per each time point studied in two independent stress conditions. BDNF, brain-derived neurotrophic factor; CMRS, chronic mild repeated stress.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2908888&req=5

Figure 7: Time course of BDNF protein expression in the hippocampus measured by Western blotting after chronic repeated stress. (A) Representative photographic film, illustrating the dynamic changes in BDNF protein from the young and aged CMRS groups. (B) Results of statistical analysis of BDNF protein expression in control groups (unstressed, 0 min) and stressed animals after 15, 30, 60, 180, 720 min of stress (in two stress conditions). The results were expressed as mean ± SEM. *p < 0.05 and **p < 0.001 vs. young control group. †p < 0.05 and ‡p < 0.001 vs. aged control group. n = 5-6 rats per each time point studied in two independent stress conditions. BDNF, brain-derived neurotrophic factor; CMRS, chronic mild repeated stress.
Mentions: Western blotting was performed in the hippocampus to examine discrete modifications of the BDNF protein on control groups (0 min) and stress-exposed groups at 15, 30, 60, 180, and 720 min after stress. After AS, we observed a rapid increase in BDNF at 15 min (as well as 30 and 60 min) throughout the entire hippocampus, in both young and aged groups. A decrease occurred at 180 min and the levels had approached the original condition by 720 min. The maximum expression of BDNF in both young and aged AS groups were observed at 30 min (Fig. 6). After chronic repeated stress, the expression of BDNF showed a dynamic change at 15, 30, 60, 180, and 720 min, whereas the levels were lower than those measured in both the young and aged control groups. However, we observed that the BDNF protein level detected in the young group, 15 min after the last repeated stimulus, was higher than that measured at all other time points, and displayed a remarkable change from 15 min to 180 min. In contrast, the expression level in the aged group was lower than that of the young group at several time points and showed a similarly low level from 60 min to 720 min (Fig. 7).

Bottom Line: The short AS induced a significant increase in BDNF mRNA and protein in both age groups, but the changes in the young group were substantially greater than those of the aged group (p < 0.005).The CMRS resulted in a decrease in BDNF mRNA and protein, but a significant increase in TrkB mRNA in both young and age groups.The results indicated that the up/down-regulation of BDNF and TrkB were affected by aging and the stimulus paradigm, which might reflect important mechanisms by which the hippocampus copes with stressful stimuli.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Psychology, Institute of Biochemistry and Molecular Biology, Binzhou Medical University, Yantai, Shandong, China.

ABSTRACT

Purpose: The purpose of this study is to explore the dynamic change of brainderived neurotrophic factor (BDNF) mRNA, protein, and tyrosine kinase-coupled receptor (TrkB) mRNA of the rat hippocampus under different stress conditions and to explore the influence of senescence on the productions expression.

Materials and methods: By using forced-swimming in 4 degrees C cold ice water and 25 degrees C warm water, young and aged male rats were randomly divided into acute stress (AS) and chronic mild repeated stress (CMRS) subgroups, respectively. BDNF productions and TrkB mRNA in the hippocampus were detected by using Western-blotting and reverse transcription-polymerase chain reaction (RT-PCR), separately, at 15, 30, 60, 180, and 720 min after the last stress session.

Results: The short AS induced a significant increase in BDNF mRNA and protein in both age groups, but the changes in the young group were substantially greater than those of the aged group (p < 0.005). The CMRS resulted in a decrease in BDNF mRNA and protein, but a significant increase in TrkB mRNA in both young and age groups. The expression of BDNF mRNA and protein in the AS groups were higher than in the CMRS groups at 15, 30, and 60 min after stress.

Conclusion: The results indicated that the up/down-regulation of BDNF and TrkB were affected by aging and the stimulus paradigm, which might reflect important mechanisms by which the hippocampus copes with stressful stimuli.

Show MeSH
Related in: MedlinePlus