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Acute stress and chronic stress change brain-derived neurotrophic factor (BDNF) and tyrosine kinase-coupled receptor (TrkB) expression in both young and aged rat hippocampus.

Shi SS, Shao SH, Yuan BP, Pan F, Li ZL - Yonsei Med. J. (2010)

Bottom Line: The short AS induced a significant increase in BDNF mRNA and protein in both age groups, but the changes in the young group were substantially greater than those of the aged group (p < 0.005).The CMRS resulted in a decrease in BDNF mRNA and protein, but a significant increase in TrkB mRNA in both young and age groups.The results indicated that the up/down-regulation of BDNF and TrkB were affected by aging and the stimulus paradigm, which might reflect important mechanisms by which the hippocampus copes with stressful stimuli.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Psychology, Institute of Biochemistry and Molecular Biology, Binzhou Medical University, Yantai, Shandong, China.

ABSTRACT

Purpose: The purpose of this study is to explore the dynamic change of brainderived neurotrophic factor (BDNF) mRNA, protein, and tyrosine kinase-coupled receptor (TrkB) mRNA of the rat hippocampus under different stress conditions and to explore the influence of senescence on the productions expression.

Materials and methods: By using forced-swimming in 4 degrees C cold ice water and 25 degrees C warm water, young and aged male rats were randomly divided into acute stress (AS) and chronic mild repeated stress (CMRS) subgroups, respectively. BDNF productions and TrkB mRNA in the hippocampus were detected by using Western-blotting and reverse transcription-polymerase chain reaction (RT-PCR), separately, at 15, 30, 60, 180, and 720 min after the last stress session.

Results: The short AS induced a significant increase in BDNF mRNA and protein in both age groups, but the changes in the young group were substantially greater than those of the aged group (p < 0.005). The CMRS resulted in a decrease in BDNF mRNA and protein, but a significant increase in TrkB mRNA in both young and age groups. The expression of BDNF mRNA and protein in the AS groups were higher than in the CMRS groups at 15, 30, and 60 min after stress.

Conclusion: The results indicated that the up/down-regulation of BDNF and TrkB were affected by aging and the stimulus paradigm, which might reflect important mechanisms by which the hippocampus copes with stressful stimuli.

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Related in: MedlinePlus

Temporal profile of plasma corticosterone (ng/mL) levels of young and aged AS group rats. Values are shown for control animals (0 min, n = 6) and after 15, 30, 60, 180 and 720 min of stress. The results are expressed as mean ± SEM. *p < 0.001 vs. young control group. **p < 0.001 vs. aged control group. AS, acute stress.
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Figure 4: Temporal profile of plasma corticosterone (ng/mL) levels of young and aged AS group rats. Values are shown for control animals (0 min, n = 6) and after 15, 30, 60, 180 and 720 min of stress. The results are expressed as mean ± SEM. *p < 0.001 vs. young control group. **p < 0.001 vs. aged control group. AS, acute stress.

Mentions: There was a large, rapid, and significant increase in plasma corticosterone levels after 15 min of AS, both in the young and the aged groups (294 ± 27 ng/mL vs. 21 ± 7 ng/mL in young control group, t = 42.89, p < 0.001; 197 ± 31 ng/mL vs. 9 ± 2 ng/mL in aged control group, t = 13.36, p < 0.001), and the highest peak of corticosterone present, respectively, at 30 min in young group and 60 min in aged groups after stress (466 ± 43 ng/mL; 328 ± 22 ng/mL), which all maintained high levels until 180 min after stress (Fig. 4). The levels of plasma corticosterone in the two CMRS groups also showed increases after stress, and reached maximal concentrations 30 min after stress (193 ± 38 ng/mL in young group; 104 ± 20 ng/mL in aged group), which were lower than those of AS groups (Fyoung = 26.58, p < 0.01; Faged = 25.46, p < 0.01) (Fig. 5).


Acute stress and chronic stress change brain-derived neurotrophic factor (BDNF) and tyrosine kinase-coupled receptor (TrkB) expression in both young and aged rat hippocampus.

Shi SS, Shao SH, Yuan BP, Pan F, Li ZL - Yonsei Med. J. (2010)

Temporal profile of plasma corticosterone (ng/mL) levels of young and aged AS group rats. Values are shown for control animals (0 min, n = 6) and after 15, 30, 60, 180 and 720 min of stress. The results are expressed as mean ± SEM. *p < 0.001 vs. young control group. **p < 0.001 vs. aged control group. AS, acute stress.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2908888&req=5

Figure 4: Temporal profile of plasma corticosterone (ng/mL) levels of young and aged AS group rats. Values are shown for control animals (0 min, n = 6) and after 15, 30, 60, 180 and 720 min of stress. The results are expressed as mean ± SEM. *p < 0.001 vs. young control group. **p < 0.001 vs. aged control group. AS, acute stress.
Mentions: There was a large, rapid, and significant increase in plasma corticosterone levels after 15 min of AS, both in the young and the aged groups (294 ± 27 ng/mL vs. 21 ± 7 ng/mL in young control group, t = 42.89, p < 0.001; 197 ± 31 ng/mL vs. 9 ± 2 ng/mL in aged control group, t = 13.36, p < 0.001), and the highest peak of corticosterone present, respectively, at 30 min in young group and 60 min in aged groups after stress (466 ± 43 ng/mL; 328 ± 22 ng/mL), which all maintained high levels until 180 min after stress (Fig. 4). The levels of plasma corticosterone in the two CMRS groups also showed increases after stress, and reached maximal concentrations 30 min after stress (193 ± 38 ng/mL in young group; 104 ± 20 ng/mL in aged group), which were lower than those of AS groups (Fyoung = 26.58, p < 0.01; Faged = 25.46, p < 0.01) (Fig. 5).

Bottom Line: The short AS induced a significant increase in BDNF mRNA and protein in both age groups, but the changes in the young group were substantially greater than those of the aged group (p < 0.005).The CMRS resulted in a decrease in BDNF mRNA and protein, but a significant increase in TrkB mRNA in both young and age groups.The results indicated that the up/down-regulation of BDNF and TrkB were affected by aging and the stimulus paradigm, which might reflect important mechanisms by which the hippocampus copes with stressful stimuli.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Psychology, Institute of Biochemistry and Molecular Biology, Binzhou Medical University, Yantai, Shandong, China.

ABSTRACT

Purpose: The purpose of this study is to explore the dynamic change of brainderived neurotrophic factor (BDNF) mRNA, protein, and tyrosine kinase-coupled receptor (TrkB) mRNA of the rat hippocampus under different stress conditions and to explore the influence of senescence on the productions expression.

Materials and methods: By using forced-swimming in 4 degrees C cold ice water and 25 degrees C warm water, young and aged male rats were randomly divided into acute stress (AS) and chronic mild repeated stress (CMRS) subgroups, respectively. BDNF productions and TrkB mRNA in the hippocampus were detected by using Western-blotting and reverse transcription-polymerase chain reaction (RT-PCR), separately, at 15, 30, 60, 180, and 720 min after the last stress session.

Results: The short AS induced a significant increase in BDNF mRNA and protein in both age groups, but the changes in the young group were substantially greater than those of the aged group (p < 0.005). The CMRS resulted in a decrease in BDNF mRNA and protein, but a significant increase in TrkB mRNA in both young and age groups. The expression of BDNF mRNA and protein in the AS groups were higher than in the CMRS groups at 15, 30, and 60 min after stress.

Conclusion: The results indicated that the up/down-regulation of BDNF and TrkB were affected by aging and the stimulus paradigm, which might reflect important mechanisms by which the hippocampus copes with stressful stimuli.

Show MeSH
Related in: MedlinePlus