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Acute stress and chronic stress change brain-derived neurotrophic factor (BDNF) and tyrosine kinase-coupled receptor (TrkB) expression in both young and aged rat hippocampus.

Shi SS, Shao SH, Yuan BP, Pan F, Li ZL - Yonsei Med. J. (2010)

Bottom Line: The short AS induced a significant increase in BDNF mRNA and protein in both age groups, but the changes in the young group were substantially greater than those of the aged group (p < 0.005).The CMRS resulted in a decrease in BDNF mRNA and protein, but a significant increase in TrkB mRNA in both young and age groups.The results indicated that the up/down-regulation of BDNF and TrkB were affected by aging and the stimulus paradigm, which might reflect important mechanisms by which the hippocampus copes with stressful stimuli.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Psychology, Institute of Biochemistry and Molecular Biology, Binzhou Medical University, Yantai, Shandong, China.

ABSTRACT

Purpose: The purpose of this study is to explore the dynamic change of brainderived neurotrophic factor (BDNF) mRNA, protein, and tyrosine kinase-coupled receptor (TrkB) mRNA of the rat hippocampus under different stress conditions and to explore the influence of senescence on the productions expression.

Materials and methods: By using forced-swimming in 4 degrees C cold ice water and 25 degrees C warm water, young and aged male rats were randomly divided into acute stress (AS) and chronic mild repeated stress (CMRS) subgroups, respectively. BDNF productions and TrkB mRNA in the hippocampus were detected by using Western-blotting and reverse transcription-polymerase chain reaction (RT-PCR), separately, at 15, 30, 60, 180, and 720 min after the last stress session.

Results: The short AS induced a significant increase in BDNF mRNA and protein in both age groups, but the changes in the young group were substantially greater than those of the aged group (p < 0.005). The CMRS resulted in a decrease in BDNF mRNA and protein, but a significant increase in TrkB mRNA in both young and age groups. The expression of BDNF mRNA and protein in the AS groups were higher than in the CMRS groups at 15, 30, and 60 min after stress.

Conclusion: The results indicated that the up/down-regulation of BDNF and TrkB were affected by aging and the stimulus paradigm, which might reflect important mechanisms by which the hippocampus copes with stressful stimuli.

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Related in: MedlinePlus

The results of an open field test for aged CMRS group rats (Mean ± SD) (n = 6). *p < 0.05, **p < 0.001, †p < 0.05, ‡p < 0.001, different from control groups (Student's t-test). (A) Number of square crossings in different stress periods. (B) Vertical movements in different stress periods. (C) Number of grooming events in different stress periods. (D) Time spent in the center square during a stress course. CMRS, chronic mild repeated stress.
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Figure 3: The results of an open field test for aged CMRS group rats (Mean ± SD) (n = 6). *p < 0.05, **p < 0.001, †p < 0.05, ‡p < 0.001, different from control groups (Student's t-test). (A) Number of square crossings in different stress periods. (B) Vertical movements in different stress periods. (C) Number of grooming events in different stress periods. (D) Time spent in the center square during a stress course. CMRS, chronic mild repeated stress.

Mentions: Fig. 2 shows that there were no significant differences in exploratory behaviors among handled and control animals in either age group prior to imposition of stress. With prolongation of the experimental days, the quadrant crossing, grooming, and vertical movement scores of young and aged CMRS group animals exhibited a downward trend, and the time spent in the center square clearly increased. The OF indices of the aged CMRS group showed significant decreases compared with the young group, and displayed depression-like behavior and weak stress resilience (Figs. 2 and 3).


Acute stress and chronic stress change brain-derived neurotrophic factor (BDNF) and tyrosine kinase-coupled receptor (TrkB) expression in both young and aged rat hippocampus.

Shi SS, Shao SH, Yuan BP, Pan F, Li ZL - Yonsei Med. J. (2010)

The results of an open field test for aged CMRS group rats (Mean ± SD) (n = 6). *p < 0.05, **p < 0.001, †p < 0.05, ‡p < 0.001, different from control groups (Student's t-test). (A) Number of square crossings in different stress periods. (B) Vertical movements in different stress periods. (C) Number of grooming events in different stress periods. (D) Time spent in the center square during a stress course. CMRS, chronic mild repeated stress.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2908888&req=5

Figure 3: The results of an open field test for aged CMRS group rats (Mean ± SD) (n = 6). *p < 0.05, **p < 0.001, †p < 0.05, ‡p < 0.001, different from control groups (Student's t-test). (A) Number of square crossings in different stress periods. (B) Vertical movements in different stress periods. (C) Number of grooming events in different stress periods. (D) Time spent in the center square during a stress course. CMRS, chronic mild repeated stress.
Mentions: Fig. 2 shows that there were no significant differences in exploratory behaviors among handled and control animals in either age group prior to imposition of stress. With prolongation of the experimental days, the quadrant crossing, grooming, and vertical movement scores of young and aged CMRS group animals exhibited a downward trend, and the time spent in the center square clearly increased. The OF indices of the aged CMRS group showed significant decreases compared with the young group, and displayed depression-like behavior and weak stress resilience (Figs. 2 and 3).

Bottom Line: The short AS induced a significant increase in BDNF mRNA and protein in both age groups, but the changes in the young group were substantially greater than those of the aged group (p < 0.005).The CMRS resulted in a decrease in BDNF mRNA and protein, but a significant increase in TrkB mRNA in both young and age groups.The results indicated that the up/down-regulation of BDNF and TrkB were affected by aging and the stimulus paradigm, which might reflect important mechanisms by which the hippocampus copes with stressful stimuli.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Psychology, Institute of Biochemistry and Molecular Biology, Binzhou Medical University, Yantai, Shandong, China.

ABSTRACT

Purpose: The purpose of this study is to explore the dynamic change of brainderived neurotrophic factor (BDNF) mRNA, protein, and tyrosine kinase-coupled receptor (TrkB) mRNA of the rat hippocampus under different stress conditions and to explore the influence of senescence on the productions expression.

Materials and methods: By using forced-swimming in 4 degrees C cold ice water and 25 degrees C warm water, young and aged male rats were randomly divided into acute stress (AS) and chronic mild repeated stress (CMRS) subgroups, respectively. BDNF productions and TrkB mRNA in the hippocampus were detected by using Western-blotting and reverse transcription-polymerase chain reaction (RT-PCR), separately, at 15, 30, 60, 180, and 720 min after the last stress session.

Results: The short AS induced a significant increase in BDNF mRNA and protein in both age groups, but the changes in the young group were substantially greater than those of the aged group (p < 0.005). The CMRS resulted in a decrease in BDNF mRNA and protein, but a significant increase in TrkB mRNA in both young and age groups. The expression of BDNF mRNA and protein in the AS groups were higher than in the CMRS groups at 15, 30, and 60 min after stress.

Conclusion: The results indicated that the up/down-regulation of BDNF and TrkB were affected by aging and the stimulus paradigm, which might reflect important mechanisms by which the hippocampus copes with stressful stimuli.

Show MeSH
Related in: MedlinePlus