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Acute stress and chronic stress change brain-derived neurotrophic factor (BDNF) and tyrosine kinase-coupled receptor (TrkB) expression in both young and aged rat hippocampus.

Shi SS, Shao SH, Yuan BP, Pan F, Li ZL - Yonsei Med. J. (2010)

Bottom Line: The short AS induced a significant increase in BDNF mRNA and protein in both age groups, but the changes in the young group were substantially greater than those of the aged group (p < 0.005).The CMRS resulted in a decrease in BDNF mRNA and protein, but a significant increase in TrkB mRNA in both young and age groups.The results indicated that the up/down-regulation of BDNF and TrkB were affected by aging and the stimulus paradigm, which might reflect important mechanisms by which the hippocampus copes with stressful stimuli.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Psychology, Institute of Biochemistry and Molecular Biology, Binzhou Medical University, Yantai, Shandong, China.

ABSTRACT

Purpose: The purpose of this study is to explore the dynamic change of brainderived neurotrophic factor (BDNF) mRNA, protein, and tyrosine kinase-coupled receptor (TrkB) mRNA of the rat hippocampus under different stress conditions and to explore the influence of senescence on the productions expression.

Materials and methods: By using forced-swimming in 4 degrees C cold ice water and 25 degrees C warm water, young and aged male rats were randomly divided into acute stress (AS) and chronic mild repeated stress (CMRS) subgroups, respectively. BDNF productions and TrkB mRNA in the hippocampus were detected by using Western-blotting and reverse transcription-polymerase chain reaction (RT-PCR), separately, at 15, 30, 60, 180, and 720 min after the last stress session.

Results: The short AS induced a significant increase in BDNF mRNA and protein in both age groups, but the changes in the young group were substantially greater than those of the aged group (p < 0.005). The CMRS resulted in a decrease in BDNF mRNA and protein, but a significant increase in TrkB mRNA in both young and age groups. The expression of BDNF mRNA and protein in the AS groups were higher than in the CMRS groups at 15, 30, and 60 min after stress.

Conclusion: The results indicated that the up/down-regulation of BDNF and TrkB were affected by aging and the stimulus paradigm, which might reflect important mechanisms by which the hippocampus copes with stressful stimuli.

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Related in: MedlinePlus

Diagrammatic representation of the protocols for AS and CMRS exposures. (A) The AS protocol. (B) The CMRS protocol. AS, acute stress; CMRS, chronic mild repeated stress.
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Figure 1: Diagrammatic representation of the protocols for AS and CMRS exposures. (A) The AS protocol. (B) The CMRS protocol. AS, acute stress; CMRS, chronic mild repeated stress.

Mentions: AS model: animals were forced to constantly swim in 4℃ water in a 70×40×80 cm aquarium (35 cm depth of water) for 10 minutes on the day of the experiment (Fig. 1A). CMRS model: animals were forced to swim in 25℃ water for 10 minutes at 8:30 am, which was enforced for 21 consecutive days in the same aquarium (Fig. 1B). After the conclusion of the different stress tests, animals were immediately decapitated. In the control group, unstressed animals were handled daily, and on the day of the experiment, they were sacrificed at the same time as the stressed animals.


Acute stress and chronic stress change brain-derived neurotrophic factor (BDNF) and tyrosine kinase-coupled receptor (TrkB) expression in both young and aged rat hippocampus.

Shi SS, Shao SH, Yuan BP, Pan F, Li ZL - Yonsei Med. J. (2010)

Diagrammatic representation of the protocols for AS and CMRS exposures. (A) The AS protocol. (B) The CMRS protocol. AS, acute stress; CMRS, chronic mild repeated stress.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2908888&req=5

Figure 1: Diagrammatic representation of the protocols for AS and CMRS exposures. (A) The AS protocol. (B) The CMRS protocol. AS, acute stress; CMRS, chronic mild repeated stress.
Mentions: AS model: animals were forced to constantly swim in 4℃ water in a 70×40×80 cm aquarium (35 cm depth of water) for 10 minutes on the day of the experiment (Fig. 1A). CMRS model: animals were forced to swim in 25℃ water for 10 minutes at 8:30 am, which was enforced for 21 consecutive days in the same aquarium (Fig. 1B). After the conclusion of the different stress tests, animals were immediately decapitated. In the control group, unstressed animals were handled daily, and on the day of the experiment, they were sacrificed at the same time as the stressed animals.

Bottom Line: The short AS induced a significant increase in BDNF mRNA and protein in both age groups, but the changes in the young group were substantially greater than those of the aged group (p < 0.005).The CMRS resulted in a decrease in BDNF mRNA and protein, but a significant increase in TrkB mRNA in both young and age groups.The results indicated that the up/down-regulation of BDNF and TrkB were affected by aging and the stimulus paradigm, which might reflect important mechanisms by which the hippocampus copes with stressful stimuli.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Psychology, Institute of Biochemistry and Molecular Biology, Binzhou Medical University, Yantai, Shandong, China.

ABSTRACT

Purpose: The purpose of this study is to explore the dynamic change of brainderived neurotrophic factor (BDNF) mRNA, protein, and tyrosine kinase-coupled receptor (TrkB) mRNA of the rat hippocampus under different stress conditions and to explore the influence of senescence on the productions expression.

Materials and methods: By using forced-swimming in 4 degrees C cold ice water and 25 degrees C warm water, young and aged male rats were randomly divided into acute stress (AS) and chronic mild repeated stress (CMRS) subgroups, respectively. BDNF productions and TrkB mRNA in the hippocampus were detected by using Western-blotting and reverse transcription-polymerase chain reaction (RT-PCR), separately, at 15, 30, 60, 180, and 720 min after the last stress session.

Results: The short AS induced a significant increase in BDNF mRNA and protein in both age groups, but the changes in the young group were substantially greater than those of the aged group (p < 0.005). The CMRS resulted in a decrease in BDNF mRNA and protein, but a significant increase in TrkB mRNA in both young and age groups. The expression of BDNF mRNA and protein in the AS groups were higher than in the CMRS groups at 15, 30, and 60 min after stress.

Conclusion: The results indicated that the up/down-regulation of BDNF and TrkB were affected by aging and the stimulus paradigm, which might reflect important mechanisms by which the hippocampus copes with stressful stimuli.

Show MeSH
Related in: MedlinePlus