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Bone morphogenetic protein receptor in the osteogenic differentiation of rat bone marrow stromal cells.

Wang A, Ding X, Sheng S, Yao Z - Yonsei Med. J. (2010)

Bottom Line: Bone morphogenetic protein (BMP) signaling has important effects on the process of skeletogenesis.The OM significantly induced the expression of type IA receptor of BMPR (BMPRIA) and type II receptor of BMPR (BMPRII) in BMSCs after three days of stimulation, while BMP-2 significantly induced BMPRIA and BMPRII in BMSCs after nine or six days of stimulation, respectively.In addition, BMP signaling through BMPRIA and BMPRII regulates the osteogenic differentiation of rat BMSCs in OM with or without BMP-2.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital, Sun Yat-sen University, 58 Zhongshan Road II, Guangzhou, 510080, PR China. anxunwang@yahoo.com

ABSTRACT

Purpose: Several signaling pathways have been shown to regulate the lineage commitment and terminal differentiation of bone marrow stromal cells (BMSCs). Bone morphogenetic protein (BMP) signaling has important effects on the process of skeletogenesis. In the present study, we tested the role of bone morphogenetic protein receptor (BMPR) in the osteogenic differentiation of rat bone marrow stromal cells in osteogenic medium (OM) with or without BMP-2.

Materials and methods: BMSCs were harvested from rats and cultured in OM containing dexamethasone, beta-glycerophosphate, and ascorbic acid, with or without BMP-2 in order to induce osteogenic differentiation. The alkaline phosphatase (ALP) activity assay and von kossa staining were used to assess the osteogenic differentiation of the BMSCs. BMPR mRNA expression was assessed using reverse transcriptionpolymerase chain reaction (RT-PCR).

Results: The BMSCs that underwent osteogenic differentiation in OM showed a higher level of ALP activity and matrix mineralization. BMP-2 alone induced a low level of ALP activity and matrix mineralization in BMSCs, but enhanced the osteogenic differentiation of BMSCs when combined with OM. The OM significantly induced the expression of type IA receptor of BMPR (BMPRIA) and type II receptor of BMPR (BMPRII) in BMSCs after three days of stimulation, while BMP-2 significantly induced BMPRIA and BMPRII in BMSCs after nine or six days of stimulation, respectively.

Conclusion: BMSCs commit to osteoblastic differentiation in OM, which is enhanced by BMP-2. In addition, BMP signaling through BMPRIA and BMPRII regulates the osteogenic differentiation of rat BMSCs in OM with or without BMP-2.

Show MeSH
Matrix mineralization of BMSCs in osteogenic medium with or without BMP-2. Von kossa staining of BMSCs was performed. BMSCs showed a high level of matrix mineralization when cultured in the presence of OM with or without BMP-2. No matrix mineralization was observed in cells cultured in the control medium. A low level of matrix mineralization was found in cells cultured in the presence of BMP-2 (×100). BMP-2, bone morphogenetic protein-2; OM, osteogenic medium; BMSCs, bone marrow stromal cells.
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Figure 3: Matrix mineralization of BMSCs in osteogenic medium with or without BMP-2. Von kossa staining of BMSCs was performed. BMSCs showed a high level of matrix mineralization when cultured in the presence of OM with or without BMP-2. No matrix mineralization was observed in cells cultured in the control medium. A low level of matrix mineralization was found in cells cultured in the presence of BMP-2 (×100). BMP-2, bone morphogenetic protein-2; OM, osteogenic medium; BMSCs, bone marrow stromal cells.

Mentions: BMSCs were examined for their ability to undergo matrix mineralization when cultured in OM with or without BMP-2 using von kossa staining (Fig. 3). BMSCs showed a high level of matrix mineralization when cultured in the presence of OM with or without BMP-2. BMSCs did not show any matrix mineralization when cultured in CM, and a low level of matrix mineralization was found in the presence of BMP-2.


Bone morphogenetic protein receptor in the osteogenic differentiation of rat bone marrow stromal cells.

Wang A, Ding X, Sheng S, Yao Z - Yonsei Med. J. (2010)

Matrix mineralization of BMSCs in osteogenic medium with or without BMP-2. Von kossa staining of BMSCs was performed. BMSCs showed a high level of matrix mineralization when cultured in the presence of OM with or without BMP-2. No matrix mineralization was observed in cells cultured in the control medium. A low level of matrix mineralization was found in cells cultured in the presence of BMP-2 (×100). BMP-2, bone morphogenetic protein-2; OM, osteogenic medium; BMSCs, bone marrow stromal cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2908870&req=5

Figure 3: Matrix mineralization of BMSCs in osteogenic medium with or without BMP-2. Von kossa staining of BMSCs was performed. BMSCs showed a high level of matrix mineralization when cultured in the presence of OM with or without BMP-2. No matrix mineralization was observed in cells cultured in the control medium. A low level of matrix mineralization was found in cells cultured in the presence of BMP-2 (×100). BMP-2, bone morphogenetic protein-2; OM, osteogenic medium; BMSCs, bone marrow stromal cells.
Mentions: BMSCs were examined for their ability to undergo matrix mineralization when cultured in OM with or without BMP-2 using von kossa staining (Fig. 3). BMSCs showed a high level of matrix mineralization when cultured in the presence of OM with or without BMP-2. BMSCs did not show any matrix mineralization when cultured in CM, and a low level of matrix mineralization was found in the presence of BMP-2.

Bottom Line: Bone morphogenetic protein (BMP) signaling has important effects on the process of skeletogenesis.The OM significantly induced the expression of type IA receptor of BMPR (BMPRIA) and type II receptor of BMPR (BMPRII) in BMSCs after three days of stimulation, while BMP-2 significantly induced BMPRIA and BMPRII in BMSCs after nine or six days of stimulation, respectively.In addition, BMP signaling through BMPRIA and BMPRII regulates the osteogenic differentiation of rat BMSCs in OM with or without BMP-2.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital, Sun Yat-sen University, 58 Zhongshan Road II, Guangzhou, 510080, PR China. anxunwang@yahoo.com

ABSTRACT

Purpose: Several signaling pathways have been shown to regulate the lineage commitment and terminal differentiation of bone marrow stromal cells (BMSCs). Bone morphogenetic protein (BMP) signaling has important effects on the process of skeletogenesis. In the present study, we tested the role of bone morphogenetic protein receptor (BMPR) in the osteogenic differentiation of rat bone marrow stromal cells in osteogenic medium (OM) with or without BMP-2.

Materials and methods: BMSCs were harvested from rats and cultured in OM containing dexamethasone, beta-glycerophosphate, and ascorbic acid, with or without BMP-2 in order to induce osteogenic differentiation. The alkaline phosphatase (ALP) activity assay and von kossa staining were used to assess the osteogenic differentiation of the BMSCs. BMPR mRNA expression was assessed using reverse transcriptionpolymerase chain reaction (RT-PCR).

Results: The BMSCs that underwent osteogenic differentiation in OM showed a higher level of ALP activity and matrix mineralization. BMP-2 alone induced a low level of ALP activity and matrix mineralization in BMSCs, but enhanced the osteogenic differentiation of BMSCs when combined with OM. The OM significantly induced the expression of type IA receptor of BMPR (BMPRIA) and type II receptor of BMPR (BMPRII) in BMSCs after three days of stimulation, while BMP-2 significantly induced BMPRIA and BMPRII in BMSCs after nine or six days of stimulation, respectively.

Conclusion: BMSCs commit to osteoblastic differentiation in OM, which is enhanced by BMP-2. In addition, BMP signaling through BMPRIA and BMPRII regulates the osteogenic differentiation of rat BMSCs in OM with or without BMP-2.

Show MeSH