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Bone morphogenetic protein receptor in the osteogenic differentiation of rat bone marrow stromal cells.

Wang A, Ding X, Sheng S, Yao Z - Yonsei Med. J. (2010)

Bottom Line: Bone morphogenetic protein (BMP) signaling has important effects on the process of skeletogenesis.The OM significantly induced the expression of type IA receptor of BMPR (BMPRIA) and type II receptor of BMPR (BMPRII) in BMSCs after three days of stimulation, while BMP-2 significantly induced BMPRIA and BMPRII in BMSCs after nine or six days of stimulation, respectively.In addition, BMP signaling through BMPRIA and BMPRII regulates the osteogenic differentiation of rat BMSCs in OM with or without BMP-2.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital, Sun Yat-sen University, 58 Zhongshan Road II, Guangzhou, 510080, PR China. anxunwang@yahoo.com

ABSTRACT

Purpose: Several signaling pathways have been shown to regulate the lineage commitment and terminal differentiation of bone marrow stromal cells (BMSCs). Bone morphogenetic protein (BMP) signaling has important effects on the process of skeletogenesis. In the present study, we tested the role of bone morphogenetic protein receptor (BMPR) in the osteogenic differentiation of rat bone marrow stromal cells in osteogenic medium (OM) with or without BMP-2.

Materials and methods: BMSCs were harvested from rats and cultured in OM containing dexamethasone, beta-glycerophosphate, and ascorbic acid, with or without BMP-2 in order to induce osteogenic differentiation. The alkaline phosphatase (ALP) activity assay and von kossa staining were used to assess the osteogenic differentiation of the BMSCs. BMPR mRNA expression was assessed using reverse transcriptionpolymerase chain reaction (RT-PCR).

Results: The BMSCs that underwent osteogenic differentiation in OM showed a higher level of ALP activity and matrix mineralization. BMP-2 alone induced a low level of ALP activity and matrix mineralization in BMSCs, but enhanced the osteogenic differentiation of BMSCs when combined with OM. The OM significantly induced the expression of type IA receptor of BMPR (BMPRIA) and type II receptor of BMPR (BMPRII) in BMSCs after three days of stimulation, while BMP-2 significantly induced BMPRIA and BMPRII in BMSCs after nine or six days of stimulation, respectively.

Conclusion: BMSCs commit to osteoblastic differentiation in OM, which is enhanced by BMP-2. In addition, BMP signaling through BMPRIA and BMPRII regulates the osteogenic differentiation of rat BMSCs in OM with or without BMP-2.

Show MeSH
Proliferation of BMSCs in osteogenic medium with or without BMP-2. Proliferation profiles of BMSCs cultured for 14 d in the presence of OM with or without BMP-2 were obtained. Although the cell numbers in these four groups were not significantly different (p > 0.05), cell growth was slightly inhibited in the presence of OM with or without BMP-2. CM, control medium; OM, osteogenic medium; BMSCs, bone marrow stromal cells; BMP-2, bone morphogenetic protein-2.
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Figure 1: Proliferation of BMSCs in osteogenic medium with or without BMP-2. Proliferation profiles of BMSCs cultured for 14 d in the presence of OM with or without BMP-2 were obtained. Although the cell numbers in these four groups were not significantly different (p > 0.05), cell growth was slightly inhibited in the presence of OM with or without BMP-2. CM, control medium; OM, osteogenic medium; BMSCs, bone marrow stromal cells; BMP-2, bone morphogenetic protein-2.

Mentions: The proliferation assay was performed using four different media conditions, including CM, BMP-2, OM, and OM + BMP-2. Cell numbers increased six- to ten-fold under all four conditions after two weeks of culture. Although cell numbers for these four groups were not significantly different (p > 0.05), the osteogenic medium with or without BMP-2 slightly inhibited the proliferation of BMSCs (Fig. 1).


Bone morphogenetic protein receptor in the osteogenic differentiation of rat bone marrow stromal cells.

Wang A, Ding X, Sheng S, Yao Z - Yonsei Med. J. (2010)

Proliferation of BMSCs in osteogenic medium with or without BMP-2. Proliferation profiles of BMSCs cultured for 14 d in the presence of OM with or without BMP-2 were obtained. Although the cell numbers in these four groups were not significantly different (p > 0.05), cell growth was slightly inhibited in the presence of OM with or without BMP-2. CM, control medium; OM, osteogenic medium; BMSCs, bone marrow stromal cells; BMP-2, bone morphogenetic protein-2.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2908870&req=5

Figure 1: Proliferation of BMSCs in osteogenic medium with or without BMP-2. Proliferation profiles of BMSCs cultured for 14 d in the presence of OM with or without BMP-2 were obtained. Although the cell numbers in these four groups were not significantly different (p > 0.05), cell growth was slightly inhibited in the presence of OM with or without BMP-2. CM, control medium; OM, osteogenic medium; BMSCs, bone marrow stromal cells; BMP-2, bone morphogenetic protein-2.
Mentions: The proliferation assay was performed using four different media conditions, including CM, BMP-2, OM, and OM + BMP-2. Cell numbers increased six- to ten-fold under all four conditions after two weeks of culture. Although cell numbers for these four groups were not significantly different (p > 0.05), the osteogenic medium with or without BMP-2 slightly inhibited the proliferation of BMSCs (Fig. 1).

Bottom Line: Bone morphogenetic protein (BMP) signaling has important effects on the process of skeletogenesis.The OM significantly induced the expression of type IA receptor of BMPR (BMPRIA) and type II receptor of BMPR (BMPRII) in BMSCs after three days of stimulation, while BMP-2 significantly induced BMPRIA and BMPRII in BMSCs after nine or six days of stimulation, respectively.In addition, BMP signaling through BMPRIA and BMPRII regulates the osteogenic differentiation of rat BMSCs in OM with or without BMP-2.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital, Sun Yat-sen University, 58 Zhongshan Road II, Guangzhou, 510080, PR China. anxunwang@yahoo.com

ABSTRACT

Purpose: Several signaling pathways have been shown to regulate the lineage commitment and terminal differentiation of bone marrow stromal cells (BMSCs). Bone morphogenetic protein (BMP) signaling has important effects on the process of skeletogenesis. In the present study, we tested the role of bone morphogenetic protein receptor (BMPR) in the osteogenic differentiation of rat bone marrow stromal cells in osteogenic medium (OM) with or without BMP-2.

Materials and methods: BMSCs were harvested from rats and cultured in OM containing dexamethasone, beta-glycerophosphate, and ascorbic acid, with or without BMP-2 in order to induce osteogenic differentiation. The alkaline phosphatase (ALP) activity assay and von kossa staining were used to assess the osteogenic differentiation of the BMSCs. BMPR mRNA expression was assessed using reverse transcriptionpolymerase chain reaction (RT-PCR).

Results: The BMSCs that underwent osteogenic differentiation in OM showed a higher level of ALP activity and matrix mineralization. BMP-2 alone induced a low level of ALP activity and matrix mineralization in BMSCs, but enhanced the osteogenic differentiation of BMSCs when combined with OM. The OM significantly induced the expression of type IA receptor of BMPR (BMPRIA) and type II receptor of BMPR (BMPRII) in BMSCs after three days of stimulation, while BMP-2 significantly induced BMPRIA and BMPRII in BMSCs after nine or six days of stimulation, respectively.

Conclusion: BMSCs commit to osteoblastic differentiation in OM, which is enhanced by BMP-2. In addition, BMP signaling through BMPRIA and BMPRII regulates the osteogenic differentiation of rat BMSCs in OM with or without BMP-2.

Show MeSH