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Propofol and aminophylline antagonize each other during the mobilization of intracellular calcium in human umbilical vein endothelial cells.

Son HJ, Lim YC, Ha KS, Kang SS, Cheong IY, Lee SJ, Park SW, Hwang BM - J. Korean Med. Sci. (2010)

Bottom Line: The results were expressed as relative fluorescence intensity and fold stimulation.Propofol further prevented the aminophylline-induced increase of intracellular calcium at clinically relevant concentrations.However, aminophylline reversed the inhibitory effect of propofol on the elevation of intracellular calcium by lysophosphatidic acid.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology and Pain Medicine, Kangwon National University Medical School, Chuncheon, Korea.

ABSTRACT
This study examined whether propofol and aminophylline affect the mobilization of intracellular calcium in human umbilical vein endothelial cells. Intracellular calcium was measured using laser scanning confocal microscopy. Cultured and serum-starved cells on round coverslips were incubated with propofol or aminophylline for 30 min, and then stimulated with lysophosphatidic acid, propofol and aminophylline. The results were expressed as relative fluorescence intensity and fold stimulation. Propofol decreased the concentration of intracellular calcium, whereas aminophylline caused increased mobilization of intracellular calcium in a concentration-dependent manner. Propofol suppressed the lysophosphatidic acid-induced mobilization of intracellular calcium in a concentration-dependent manner. Propofol further prevented the aminophylline-induced increase of intracellular calcium at clinically relevant concentrations. However, aminophylline reversed the inhibitory effect of propofol on the elevation of intracellular calcium by lysophosphatidic acid. Our results suggest that propofol and aminophylline antagonize each other on the mobilization of intracellular calcium in human umbilical vein endothelial cells at clinically relevant concentrations. Serious consideration should be given to how this interaction affects mobilization of intracellular calcium when these two drugs are used together.

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Fluorescence of intracellular calcium in human umbilical-vein endothelial cells (HUVECs) incubated with Fluo-4 and detected by a fluorescence spectrophotometer (confocal microscope). (A) Control group (resting cells) before treatment. (B) Preincubation with propofol (300 µM) blocks lysophosphatidic acid (LPA) signals. (C) Aminophylline (1,000 µM) treatment increases Fluo-4 fluorescence. (D) LPA (5 µg/mL) increases Fluo-4 fluorescence.
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Figure 1: Fluorescence of intracellular calcium in human umbilical-vein endothelial cells (HUVECs) incubated with Fluo-4 and detected by a fluorescence spectrophotometer (confocal microscope). (A) Control group (resting cells) before treatment. (B) Preincubation with propofol (300 µM) blocks lysophosphatidic acid (LPA) signals. (C) Aminophylline (1,000 µM) treatment increases Fluo-4 fluorescence. (D) LPA (5 µg/mL) increases Fluo-4 fluorescence.

Mentions: The fluorescence intensity in untreated HUVECs loaded with Fluo-4 was very low and distributed homogeneously throughout the cells (Fig. 1A). Fluo-4 fluorescence in cells treated with 5 µg/mL of LPA or 1,000 µM of aminophylline increased rapidly (Fig. 1C, D). Propofol (300 µM) blocked the increase induced by LPA (5 µg/mL) (Fig. 1B). HUVECs treated with LPA (5 µg/mL) showed a very rapid increase in [Ca2+]i (Fig. 2A), but propofol blocked this increase dose-dependently at clinically relevant concentrations (10, 30 µM) (Fig. 2B).


Propofol and aminophylline antagonize each other during the mobilization of intracellular calcium in human umbilical vein endothelial cells.

Son HJ, Lim YC, Ha KS, Kang SS, Cheong IY, Lee SJ, Park SW, Hwang BM - J. Korean Med. Sci. (2010)

Fluorescence of intracellular calcium in human umbilical-vein endothelial cells (HUVECs) incubated with Fluo-4 and detected by a fluorescence spectrophotometer (confocal microscope). (A) Control group (resting cells) before treatment. (B) Preincubation with propofol (300 µM) blocks lysophosphatidic acid (LPA) signals. (C) Aminophylline (1,000 µM) treatment increases Fluo-4 fluorescence. (D) LPA (5 µg/mL) increases Fluo-4 fluorescence.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2908795&req=5

Figure 1: Fluorescence of intracellular calcium in human umbilical-vein endothelial cells (HUVECs) incubated with Fluo-4 and detected by a fluorescence spectrophotometer (confocal microscope). (A) Control group (resting cells) before treatment. (B) Preincubation with propofol (300 µM) blocks lysophosphatidic acid (LPA) signals. (C) Aminophylline (1,000 µM) treatment increases Fluo-4 fluorescence. (D) LPA (5 µg/mL) increases Fluo-4 fluorescence.
Mentions: The fluorescence intensity in untreated HUVECs loaded with Fluo-4 was very low and distributed homogeneously throughout the cells (Fig. 1A). Fluo-4 fluorescence in cells treated with 5 µg/mL of LPA or 1,000 µM of aminophylline increased rapidly (Fig. 1C, D). Propofol (300 µM) blocked the increase induced by LPA (5 µg/mL) (Fig. 1B). HUVECs treated with LPA (5 µg/mL) showed a very rapid increase in [Ca2+]i (Fig. 2A), but propofol blocked this increase dose-dependently at clinically relevant concentrations (10, 30 µM) (Fig. 2B).

Bottom Line: The results were expressed as relative fluorescence intensity and fold stimulation.Propofol further prevented the aminophylline-induced increase of intracellular calcium at clinically relevant concentrations.However, aminophylline reversed the inhibitory effect of propofol on the elevation of intracellular calcium by lysophosphatidic acid.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology and Pain Medicine, Kangwon National University Medical School, Chuncheon, Korea.

ABSTRACT
This study examined whether propofol and aminophylline affect the mobilization of intracellular calcium in human umbilical vein endothelial cells. Intracellular calcium was measured using laser scanning confocal microscopy. Cultured and serum-starved cells on round coverslips were incubated with propofol or aminophylline for 30 min, and then stimulated with lysophosphatidic acid, propofol and aminophylline. The results were expressed as relative fluorescence intensity and fold stimulation. Propofol decreased the concentration of intracellular calcium, whereas aminophylline caused increased mobilization of intracellular calcium in a concentration-dependent manner. Propofol suppressed the lysophosphatidic acid-induced mobilization of intracellular calcium in a concentration-dependent manner. Propofol further prevented the aminophylline-induced increase of intracellular calcium at clinically relevant concentrations. However, aminophylline reversed the inhibitory effect of propofol on the elevation of intracellular calcium by lysophosphatidic acid. Our results suggest that propofol and aminophylline antagonize each other on the mobilization of intracellular calcium in human umbilical vein endothelial cells at clinically relevant concentrations. Serious consideration should be given to how this interaction affects mobilization of intracellular calcium when these two drugs are used together.

Show MeSH