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A novel statin-mediated "prenylation block-and-release" assay provides insight into the membrane targeting mechanisms of small GTPases.

Ali BR, Nouvel I, Leung KF, Hume AN, Seabra MC - Biochem. Biophys. Res. Commun. (2010)

Bottom Line: To allow association with membranes Ras-related GTPases are post-translationally modified by covalent attachment of prenyl groups to conserved cysteine residues at or near their C-terminus.We found that all Rabs tested were targeted directly to the membrane on which they reside at steady-state and not via an intermediate location as reported for Ras and Rho proteins.However, we observed that the kinetics of cytosol to membrane targeting differed for each Rab tested.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Faculty of Medicine and Health Sciences, United Arab Emirates University, Al-Ain, United Arab Emirates. bassam.ali@uaeu.ac.ae

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Still images from movies of time-course of Rab membrane targeting following mevastatin prenylation block. HeLa cells expressing EGFP-Rab5a (A) or EGFP-Rab1a (B) in the presence of mevastatin were washed with mevastatin-free medium and images collected at the indicated times (in minutes) following removal of the inhibitor. Images shown in A and B correspond to movies 1 and 2, respectively. Bars = 10 μm.
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d32e579: Still images from movies of time-course of Rab membrane targeting following mevastatin prenylation block. HeLa cells expressing EGFP-Rab5a (A) or EGFP-Rab1a (B) in the presence of mevastatin were washed with mevastatin-free medium and images collected at the indicated times (in minutes) following removal of the inhibitor. Images shown in A and B correspond to movies 1 and 2, respectively. Bars = 10 μm.


A novel statin-mediated "prenylation block-and-release" assay provides insight into the membrane targeting mechanisms of small GTPases.

Ali BR, Nouvel I, Leung KF, Hume AN, Seabra MC - Biochem. Biophys. Res. Commun. (2010)

Still images from movies of time-course of Rab membrane targeting following mevastatin prenylation block. HeLa cells expressing EGFP-Rab5a (A) or EGFP-Rab1a (B) in the presence of mevastatin were washed with mevastatin-free medium and images collected at the indicated times (in minutes) following removal of the inhibitor. Images shown in A and B correspond to movies 1 and 2, respectively. Bars = 10 μm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2908739&req=5

d32e579: Still images from movies of time-course of Rab membrane targeting following mevastatin prenylation block. HeLa cells expressing EGFP-Rab5a (A) or EGFP-Rab1a (B) in the presence of mevastatin were washed with mevastatin-free medium and images collected at the indicated times (in minutes) following removal of the inhibitor. Images shown in A and B correspond to movies 1 and 2, respectively. Bars = 10 μm.
Bottom Line: To allow association with membranes Ras-related GTPases are post-translationally modified by covalent attachment of prenyl groups to conserved cysteine residues at or near their C-terminus.We found that all Rabs tested were targeted directly to the membrane on which they reside at steady-state and not via an intermediate location as reported for Ras and Rho proteins.However, we observed that the kinetics of cytosol to membrane targeting differed for each Rab tested.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Faculty of Medicine and Health Sciences, United Arab Emirates University, Al-Ain, United Arab Emirates. bassam.ali@uaeu.ac.ae

Show MeSH
Related in: MedlinePlus