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A collection of target mimics for comprehensive analysis of microRNA function in Arabidopsis thaliana.

Todesco M, Rubio-Somoza I, Paz-Ares J, Weigel D - PLoS Genet. (2010)

Bottom Line: Morphological defects in the aerial part were observed for approximately 20% of analyzed families, all of which are deeply conserved in land plants.That less conserved miRNAs rarely had obvious effects on plant morphology suggests that most of them do not affect fundamental aspects of development.In addition to insight into modes of miRNA action, this study provides an important resource for the study of miRNA function in plants.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology, Max Planck Institute for Developmental Biology, Tübingen, Germany.

ABSTRACT
Many targets of plant microRNAs (miRNAs) are thought to play important roles in plant physiology and development. However, because plant miRNAs are typically encoded by medium-size gene families, it has often been difficult to assess their precise function. We report the generation of a large-scale collection of knockdowns for Arabidopsis thaliana miRNA families; this has been achieved using artificial miRNA target mimics, a recently developed technique fashioned on an endogenous mechanism of miRNA regulation. Morphological defects in the aerial part were observed for approximately 20% of analyzed families, all of which are deeply conserved in land plants. In addition, we find that non-cleavable mimic sites can confer translational regulation in cis. Phenotypes of plants expressing target mimics directed against miRNAs involved in development were in several cases consistent with previous reports on plants expressing miRNA-resistant forms of individual target genes, indicating that a limited number of targets mediates most effects of these miRNAs. That less conserved miRNAs rarely had obvious effects on plant morphology suggests that most of them do not affect fundamental aspects of development. In addition to insight into modes of miRNA action, this study provides an important resource for the study of miRNA function in plants.

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Leaf rosettes of target mimic expressing plants.Three-week-old plants. Bar corresponds to 1 cm for all panels.
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pgen-1001031-g002: Leaf rosettes of target mimic expressing plants.Three-week-old plants. Bar corresponds to 1 cm for all panels.

Mentions: MIM156 and MIM157 plants (Figure 2) had reduced leaf initiation rates, such that they flowered at about the same time as wild type, but with only two or three true leaves. This phenotype is similar to what is seen in plants carrying non-targetable versions of SPL9 or SPL10, two of the miR156/157 targets, and opposite of plants overexpressing miR156b or spl9 spl15 double mutants [10], [40]–[42]. In addition, these plants had bent, spoon-shaped cotyledons. The few rosette leaves were characterized by serrated margins, indicating adult leaf identity, consistent with a role of miR156 and its targets in controlling phase change [30].


A collection of target mimics for comprehensive analysis of microRNA function in Arabidopsis thaliana.

Todesco M, Rubio-Somoza I, Paz-Ares J, Weigel D - PLoS Genet. (2010)

Leaf rosettes of target mimic expressing plants.Three-week-old plants. Bar corresponds to 1 cm for all panels.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2908682&req=5

pgen-1001031-g002: Leaf rosettes of target mimic expressing plants.Three-week-old plants. Bar corresponds to 1 cm for all panels.
Mentions: MIM156 and MIM157 plants (Figure 2) had reduced leaf initiation rates, such that they flowered at about the same time as wild type, but with only two or three true leaves. This phenotype is similar to what is seen in plants carrying non-targetable versions of SPL9 or SPL10, two of the miR156/157 targets, and opposite of plants overexpressing miR156b or spl9 spl15 double mutants [10], [40]–[42]. In addition, these plants had bent, spoon-shaped cotyledons. The few rosette leaves were characterized by serrated margins, indicating adult leaf identity, consistent with a role of miR156 and its targets in controlling phase change [30].

Bottom Line: Morphological defects in the aerial part were observed for approximately 20% of analyzed families, all of which are deeply conserved in land plants.That less conserved miRNAs rarely had obvious effects on plant morphology suggests that most of them do not affect fundamental aspects of development.In addition to insight into modes of miRNA action, this study provides an important resource for the study of miRNA function in plants.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology, Max Planck Institute for Developmental Biology, Tübingen, Germany.

ABSTRACT
Many targets of plant microRNAs (miRNAs) are thought to play important roles in plant physiology and development. However, because plant miRNAs are typically encoded by medium-size gene families, it has often been difficult to assess their precise function. We report the generation of a large-scale collection of knockdowns for Arabidopsis thaliana miRNA families; this has been achieved using artificial miRNA target mimics, a recently developed technique fashioned on an endogenous mechanism of miRNA regulation. Morphological defects in the aerial part were observed for approximately 20% of analyzed families, all of which are deeply conserved in land plants. In addition, we find that non-cleavable mimic sites can confer translational regulation in cis. Phenotypes of plants expressing target mimics directed against miRNAs involved in development were in several cases consistent with previous reports on plants expressing miRNA-resistant forms of individual target genes, indicating that a limited number of targets mediates most effects of these miRNAs. That less conserved miRNAs rarely had obvious effects on plant morphology suggests that most of them do not affect fundamental aspects of development. In addition to insight into modes of miRNA action, this study provides an important resource for the study of miRNA function in plants.

Show MeSH